APRT Antibody - #DF7350

(3)  
Product: APRT Antibody
Catalog: DF7350
Description: Rabbit polyclonal antibody to APRT
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 19kDa; 20kD(Calculated).
Uniprot: P07741
RRID: AB_2839288

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(83%), Bovine(83%), Horse(92%), Sheep(92%), Dog(83%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
APRT Antibody detects endogenous levels of total APRT.
RRID:
AB_2839288
Cite Format: Affinity Biosciences Cat# DF7350, RRID:AB_2839288.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adenine phosphoribosyltransferase; AMP; AMP diphosphorylase; AMP pyrophosphorylase; APRT; APT_HUMAN; DKFZp686D13177; MGC125856; MGC125857; MGC129961; Transphosphoribosidase;

Immunogens

Immunogen:
Uniprot:

P07741(APT_HUMAN) >>Visit HPA database.

Gene(ID):
APRT(353)
Description:
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
Sequence:
MADSELQLVEQRIRSFPDFPTPGVVFRDISPVLKDPASFRAAIGLLARHLKATHGGRIDYIAGLDSRGFLFGPSLAQELGLGCVLIRKRGKLPGPTLWASYSLEYGKAELEIQKDALEPGQRVVVVDDLLATGGTMNAACELLGRLQAEVLECVSLVELTSLKGREKLAPVPFFSLLQYE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Sheep
92
Chicken
86
Bovine
83
Dog
83
Zebrafish
83
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07741 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S4 Phosphorylation
S15 Phosphorylation
T21 Phosphorylation
S30 Phosphorylation
K34 Ubiquitination
S38 Phosphorylation
K51 Ubiquitination
R57 Methylation
Y60 Phosphorylation
S66 Phosphorylation
T96 Phosphorylation
S100 Phosphorylation
K114 Acetylation
K114 Ubiquitination
T135 Phosphorylation
K167 Ubiquitination

Research Backgrounds

Function:

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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