KIF2C Antibody - #DF7348

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Product: KIF2C Antibody
Catalog: DF7348
Description: Rabbit polyclonal antibody to KIF2C
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 81kDa; 81kD(Calculated).
Uniprot: Q99661
RRID: AB_2839286

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
KIF2C Antibody detects endogenous levels of total KIF2C.
RRID:
AB_2839286
Cite Format: Affinity Biosciences Cat# DF7348, RRID:AB_2839286.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4930402F02Rik; CT139; ESTM5; KIF 2C; kif2c; KIF2C_HUMAN; Kinesein Family Member 2C; Kinesin family member 2C; kinesin like 6 (mitotic centromere associated kinesin); Kinesin like 6; Kinesin like protein 6; Kinesin like protein KIF2C; Kinesin-like protein 6; Kinesin-like protein KIF2C; KNS L6; KNSL 6; KNSL6; MCAK; MGC11883; Mitotic centromere associated kinesin; Mitotic centromere-associated kinesin; OTTHUMP00000010066; X83316;

Immunogens

Immunogen:
Uniprot:

Q99661(KIF2C_HUMAN) >>Visit HPA database.

Gene(ID):
KIF2C(11004)
Expression:
Q99661 KIF2C_HUMAN:

Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.

Description:
The protein encoded by this gene is a member of kinesin-like protein family. Proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation.
Sequence:
MAMDSSLQARLFPGLAIKIQRSNGLIHSANVRTVNLEKSCVSVEWAEGGATKGKEIDFDDVAAINPELLQLLPLHPKDNLPLQENVTIQKQKRRSVNSKIPAPKESLRSRSTRMSTVSELRITAQENDMEVELPAAANSRKQFSVPPAPTRPSCPAVAEIPLRMVSEEMEEQVHSIRGSSSANPVNSVRRKSCLVKEVEKMKNKREEKKAQNSEMRMKRAQEYDSSFPNWEFARMIKEFRATLECHPLTMTDPIEEHRICVCVRKRPLNKQELAKKEIDVISIPSKCLLLVHEPKLKVDLTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKAQNASKGIYAMASRDVFLLKNQPCYRKLGLEVYVTFFEIYNGKLFDLLNKKAKLRVLEDGKQQVQVVGLQEHLVNSADDVIKMIDMGSACRTSGQTFANSNSSRSHACFQIILRAKGRMHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIATISPGISSCEYTLNTLRYADRVKELSPHSGPSGEQLIQMETEEMEACSNGALIPGNLSKEEEELSSQMSSFNEAMTQIRELEEKAMEELKEIIQQGPDWLELSEMTEQPDYDLETFVNKAESALAQQAKHFSALRDVIKALRLAMQLEEQASRQISSKKRPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
92
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99661 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S6 Phosphorylation
K18 Ubiquitination
S22 Phosphorylation
T33 Phosphorylation
K52 Ubiquitination
K54 Ubiquitination
K77 Ubiquitination
T87 Phosphorylation
K90 Ubiquitination
S95 Phosphorylation Q96GD4 (AURKB)
K99 Ubiquitination
K104 Ubiquitination
S106 Phosphorylation
S109 Phosphorylation Q96GD4 (AURKB)
S111 Phosphorylation Q13153 (PAK1) , Q96GD4 (AURKB)
T112 Phosphorylation
S115 Phosphorylation Q96GD4 (AURKB)
T116 Phosphorylation
S139 Phosphorylation
K141 Ubiquitination
S144 Phosphorylation
T150 Phosphorylation
S153 Phosphorylation
S166 Phosphorylation
S175 Phosphorylation
R177 Methylation
S180 Phosphorylation
S187 Phosphorylation
S192 Phosphorylation Q13153 (PAK1) , Q96GD4 (AURKB)
K196 Acetylation
K196 Ubiquitination
Y223 Phosphorylation
R234 Methylation
K237 Ubiquitination
K270 Ubiquitination
K275 Acetylation
K276 Acetylation
K276 Ubiquitination
K286 Ubiquitination
K297 Ubiquitination
T301 Phosphorylation
K341 Ubiquitination
K354 Ubiquitination
K365 Methylation
K365 Ubiquitination
K371 Ubiquitination
Y374 Phosphorylation
K385 Ubiquitination
Y398 Phosphorylation
Y405 Phosphorylation
K415 Ubiquitination
K426 Ubiquitination
S458 Phosphorylation
T461 Phosphorylation
K487 Ubiquitination
S489 Phosphorylation
R498 Methylation
K518 Ubiquitination
S519 Phosphorylation
K524 Ubiquitination
K534 Ubiquitination
T537 Phosphorylation P06493 (CDK1)
K543 Ubiquitination
R549 Methylation
S551 Phosphorylation
S592 Phosphorylation P53350 (PLK1)
S595 Phosphorylation P53350 (PLK1)
S611 Phosphorylation
S621 Phosphorylation P53350 (PLK1)
S628 Phosphorylation
S629 Phosphorylation
S632 Phosphorylation P53350 (PLK1)
S633 Phosphorylation P53350 (PLK1)
K647 Ubiquitination
K692 Ubiquitination
K702 Ubiquitination
S715 Phosphorylation P53350 (PLK1)
S719 Phosphorylation

Research Backgrounds

Function:

In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment.

PTMs:

Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.

Ubiquitinated.

Subcellular Location:

Cytoplasm>Cytoskeleton. Nucleus. Chromosome>Centromere. Chromosome>Centromere>Kinetochore.
Note: Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.

Subunit Structure:

Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends. Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding.

Family&Domains:

The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.

References

1). Kinesin family member 2C aggravates the progression of hepatocellular carcinoma and interacts with competing endogenous RNA. Journal of Cellular Biochemistry, 2020 (PubMed: 32056305) [IF=4.0]

Application: IHC    Species: human    Sample: tumor

FIGURE 1 |Kinesin Family Member 2C (KIF2C) is frequently elevated in tumor tissues than adjacent normal tissues. A, The mRNA level of KIF2C in pan‐cancers and adjacent normal tissues analyzed by GEPIA web tool. B, The mRNA level of KIF2C in HCC specimens indicated by GEPIA. C,D, The KIF2C expression at the status of protein level detected by Western blot assay. E, The IHC upon tissue microarrays showed the differential expression of KIF2C in HCC specimens.

Application: WB    Species: human    Sample: tumor

FIGURE 1 |Kinesin Family Member 2C (KIF2C) is frequently elevated in tumor tissues than adjacent normal tissues. A, The mRNA level of KIF2C in pan‐cancers and adjacent normal tissues analyzed by GEPIA web tool. B, The mRNA level of KIF2C in HCC specimens indicated by GEPIA. C,D, The KIF2C expression at the status of protein level detected by Western blot assay. E, The IHC upon tissue microarrays showed the differential expression of KIF2C in HCC specimens.H, The KIF2C protein level verified by Western blot assay.
2). Identification of characteristic genes distinguishing neural stem cells from astrocytes. GENE, 2019 (PubMed: 30266499) [IF=3.5]

Application: IHC    Species: mouse    Sample: brain section

Fig. 5. |Western blotting and immunohistochemistry results of KIF2C gene.(C) Representative graph of mouse E18.5 brain section by immunohistochemistry for KIF2C and SOX2. The area between white dashed lines represents the location of SVZ (VZ: ventricular zone,SVZ:subventricular zone, LV: lateral Ventricle, left bar: 60 μm, right bar: 10 μm).

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