PRKRA Antibody - #DF7334

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Product: PRKRA Antibody
Catalog: DF7334
Description: Rabbit polyclonal antibody to PRKRA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 34kDa; 34kD(Calculated).
Uniprot: O75569
RRID: AB_2839272

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PRKRA Antibody detects endogenous levels of total PRKRA.
RRID:
AB_2839272
Cite Format: Affinity Biosciences Cat# DF7334, RRID:AB_2839272.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DYT16; HSD14; Interferon inducible double stranded RNA dependent protein kinase activator A; interferon-inducible double stranded RNA-dependent activator; Interferon-inducible double stranded RNA-dependent protein kinase activator A; PACT; PKR associated protein X; PKR associating protein X; PKR-associated protein X; PKR-associating protein X; PRKRA; PRKRA_HUMAN; Protein activator of the interferon induced protein kinase; Protein activator of the interferon-induced protein kinase; Protein kinase; Protein kinase interferon inducible double stranded RNA dependent activator; RAX;

Immunogens

Immunogen:
Uniprot:

O75569(PRKRA_HUMAN) >>Visit HPA database.

Gene(ID):
PRKRA(8575)
Description:
This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants.
Sequence:
MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
57
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75569 As Substrate

Site PTM Type Enzyme
S18 Phosphorylation
T20 Phosphorylation
S22 Phosphorylation
K30 Ubiquitination
K45 Ubiquitination
K47 Ubiquitination
T67 Phosphorylation
T71 Phosphorylation
S130 Phosphorylation
K156 Ubiquitination
Y159 Phosphorylation
T160 Phosphorylation
S167 Phosphorylation
K187 Ubiquitination
S196 Phosphorylation
S202 Phosphorylation
S217 Phosphorylation
K225 Ubiquitination
S232 Phosphorylation
S235 Phosphorylation
Y241 Phosphorylation
S246 Phosphorylation
Y270 Phosphorylation
S287 Phosphorylation

Research Backgrounds

Function:

Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).

PTMs:

Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress.

Subcellular Location:

Cytoplasm>Perinuclear region. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with EIF2AK2/PKR through its DRBM domains. Interacts with DICER1, AGO2 and TARBP2. Also able to interact with dsRNA. Interacts with UBC9 (By similarity). Forms a complex with UBC9 and p53/TP53 (By similarity). Interacts with DUS2L (via DRBM domain). Interacts with DDX58.

(Microbial infection) Interacts with ebolavirus protein VP35; this interaction inhibits the interaction between DDX58 and PRKRA. In addition, this interaction disrupts the interaction between VP35 and the viral polymerase L. So the VP35-PRKRA interaction plays a critical role in determining the outcome of ebolavirus infection.

Family&Domains:

Self-association may occur via interactions between DRBM domains as follows: DRBM 1/DRBM 1, DRBM 1/DRBM 2, DRBM 2/DRBM 2 or DRBM 3/DRBM3.

Belongs to the PRKRA family.

References

1). Inhibiting the aberrant PACT-p53 axis activation ameliorates spinal cord ischaemia–reperfusion injury in rats. International Immunopharmacology, 2022 (PubMed: 35421805) [IF=5.6]
2). Novel lncRNA AL033381.2 Promotes Hepatocellular Carcinoma Progression by Upregulating PRKRA Expression. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35035655)

Application: WB    Species: Human    Sample: Huh-7 cells

Figure 5 AL033381.2 interacts with PRKRA. (a, b) The specific biotin-labeled AL033381.2 probe was added to the cell lysate, and the precipitated proteins were resolved by SDS-PAGE and then stained with silver. The AL033381.2 probe was analyzed by using SDS-PAGE. The antisense of AL033381.2 and the beads serve as the control. The AL033381.2-specific bands (red rectangle) were excised and analyzed using mass spectrometry. (c) IPA analysis identified the genes located in the cytoplasm were associated with cell proliferation and apoptosis. (d) RIP assay showed that STAU1, PRDX2, ETFB, PRKRA, KRT8, and RNH1 interacted with AL033381.2 in Huh-7 cells by RT-qPCR analysis. (e) Western blot results exhibited the capability of PRKRA, PRDX2, RNH1, and ETFB binding to AL033381.2 in Huh-7 cells. (f) RNA pull-down assay was conducted in Huh-7 cells using biotinylated AL033381.2 or antisense RNA probe transcribed in vitro and measured by western blots. (g) RIP assay was employed in Huh-7 cells using an antibody against PRKRA to determine AL033381.2 RNA enrichment in immunoprecipitated complexes with IgG as a negative control. (h) Western blot analysis was applied to determine the protein level of PRKRA after transfection of the cells with shAL033381.2 or shCtrl.

Application: IF/ICC    Species: Human    Sample: HCC cells

Figure 6 Overexpression of PRKRA rescued the cell proliferation caused by AL033381.2 knockdown in HCC cells. Celigo cell counting assay indicated that the proliferation of HCC cells was suppressed significantly in KD+NC group cells compared with NC+NC group cells, and overexpression of PRKRA (KD+OE1) rescued the inhibition of the cell proliferation induced by AL033381.2 knockdown. NC+NC group: normal target cells (Huh-7) and negative control lentivirus-infected cells (shCtrl); KD+NC group: shAL033381.2 lentivirus-infected cells (shAL033381.2) and overexpression of empty vector lentivirus-infected cells; KD+OE1 group: shAL033381.2 lentivirus-infected cells and the downstream gene PRKRA overexpressed lentivirus-infected cells; KD+OE2 group: shAL033381.2 lentivirus-infected cells and the downstream gene PRDX2 overexpressed lentivirus-infected cells; KD+OE3 group: shAL033381.2 lentivirus-infected cells and the downstream gene RNH1 overexpressed lentivirus-infected cells; KD+OE4 group: shAL033381.2 lentivirus-infected cells and the downstream gene ETFB overexpressed lentivirus-infected cells. ∗∗∗P < 0.001.

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