NPC2 Antibody - #DF7331

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Product: NPC2 Antibody
Catalog: DF7331
Description: Rabbit polyclonal antibody to NPC2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Dog
Mol.Wt.: 17kDa; 17kD(Calculated).
Uniprot: P61916
RRID: AB_2839269

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(88%), Dog(86%)
Clonality:
Polyclonal
Specificity:
NPC2 Antibody detects endogenous levels of total NPC2.
RRID:
AB_2839269
Cite Format: Affinity Biosciences Cat# DF7331, RRID:AB_2839269.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EDDM1; Epididymal protein 1; Epididymal secretory protein; Epididymal secretory protein E1; hE1; Human epididymis-specific protein 1; Niemann-Pick disease type C2; Niemann-Pick disease type C2 protein; NPC intracellular cholesterol transporter 2; NPC2; NPC2_HUMAN; Tissue specific secretory protein;

Immunogens

Immunogen:
Uniprot:

P61916(NPC2_HUMAN) >>Visit HPA database.

Gene(ID):
NPC2(10577)
Expression:
P61916 NPC2_HUMAN:

Detected in gallbladder bile (PubMed:21315718). Detected in fibroblasts, kidney, liver, spleen, small intestine, placenta and testis (at protein level) (PubMed:11125141). Epididymis.

Description:
This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy.
Sequence:
MRFLAATFLLLALSTAAQAEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVSHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Dog
86
Pig
78
Bovine
78
Sheep
78
Xenopus
78
Rabbit
78
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61916 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
S29 Phosphorylation
N58 N-Glycosylation
K105 Ubiquitination
T106 Phosphorylation
Y107 Phosphorylation
S108 Phosphorylation
K112 Ubiquitination
Y119 Phosphorylation
N135 N-Glycosylation

Research Backgrounds

Function:

Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (By similarity).

Subcellular Location:

Secreted. Endoplasmic reticulum. Lysosome.
Note: Interaction with cell-surface M6PR mediates endocytosis and targeting to lysosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in gallbladder bile. Detected in fibroblasts, kidney, liver, spleen, small intestine, placenta and testis (at protein level). Epididymis.

Subunit Structure:

Interacts with NPC1 (via the second lumenal domain) in a cholestrol-dependent manner. Interacts with NUS1/NgBR, the interaction stabilizes NCP2 and regulates cholesterol trafficking. Interacts with DHDDS. Interacts with NEDD4L (via C2 domain). Interacts with NPC1L1.

Family&Domains:

Binds cholesterol in a hydrophobic pocket; there are no hydrogen bonds between the sterol and the protein.

Belongs to the NPC2 family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Organismal Systems > Digestive system > Cholesterol metabolism.

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