NDUFA13 Antibody | Affinity Biosciences

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Western blot analysis of extracts from mouse liver ,rat brain, using NDUFA13 Antibody.

Western blot analysis of extracts from mouse kidney, using NDUFA13 Antibody.

Product:	NDUFA13 Antibody
Catalog:	DF7330
Description:	Rabbit polyclonal antibody to NDUFA13
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Dog, Xenopus
Mol.Wt.:	17kDa; 17kD(Calculated).
Uniprot:	Q9P0J0
RRID:	AB_2839268

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(92%), Bovine(100%), Dog(100%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

NDUFA13 Antibody detects endogenous levels of total NDUFA13.

RRID:

AB_2839268
Cite Format: Affinity Biosciences Cat# DF7330, RRID:AB_2839268.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2700054G14Rik; AU022060; B16.6; CDA016; Cell death regulatory protein; Cell death regulatory protein GRIM-19; CGI-39; CGI39 protein; CI-B16.6; Complex I-B16.6; FLJ58045; FLJ59191; Gene associated with retinoic and IFN-induced mortality 19 protein; Gene associated with retinoic and interferon-induced mortality 19 protein; Gene associated with retinoic interferon induced mortality 19 protein; GRIM-19; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; NADH ubiquinone oxidoreductase B16.6 subunit; NADH-ubiquinone oxidoreductase B16.6 subunit; NDUAD_HUMAN; NDUFA13; RGD1565358;

Immunogens

Immunogen:

Uniprot:

Q9P0J0(NDUAD_HUMAN) >>Visit HPA database.

Gene(ID):

NDUFA13(51079)

Expression:

Q9P0J0 NDUAD_HUMAN:

Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis.

Description:

This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined.

Sequence:

Q9P0J0 NDUAD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAASKVKQDMPPPGGYGPIDYKRNLPRRGLSGYSMLAIGIGTLIYGHWSIMKWNRERRRLQIEDFEARIALLPLLQAETDRRTLQMLRENLEEEAIIMKDVPDWKVGESVFHTTRWVPPLIGELYGLRTTEEALHASHGFMWYT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Dog

100

Xenopus

Zebrafish

Horse

Sheep

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P0J0 As Substrate

Q9P0J0

Site	PTM Type	Source
K7	Ubiquitination	Uniprot
K22	Acetylation	Uniprot
K22	Ubiquitination	Uniprot
T79	Phosphorylation	Uniprot
R81	Methylation	Uniprot
K99	Ubiquitination	Uniprot
K105	Ubiquitination	Uniprot
Y125	Phosphorylation	Uniprot

Research Backgrounds

Q9P0J0_NDUAD_HUMAN

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein>Matrix side. Nucleus.
Note: Localizes mainly in the mitochondrion (PubMed:12628925). May be translocated into the nucleus upon IFN/RA treatment.

Tissue Specificity:

Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis.

Subunit Structure:

Complex I is composed of 45 different subunits. Interacts with CARD15, but not with CARD4. Interacts with STAT3, but not with STAT1, STAT2 and STAT5A. Interacts with OLFM4.

(Microbial infection) Interacts with HHV-8 IRF1, in the nucleus, with HPV-16 E6 and SV40 LT.

Family&Domains:

Belongs to the complex I NDUFA13 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

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NDUFA13 Antibody - #DF7330