Product: MPG Antibody
Catalog: DF7328
Description: Rabbit polyclonal antibody to MPG
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 32kDa; 33kD(Calculated).
Uniprot: P29372
RRID: AB_2839266

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(83%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MPG Antibody detects endogenous levels of total MPG.
RRID:
AB_2839266
Cite Format: Affinity Biosciences Cat# DF7328, RRID:AB_2839266.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3 alkyladenine DNA glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; 3MG_HUMAN; AAG; ADPG; Alkyladenine DNA glycosylase; anpg; APNG; CRA36.1; DNA 3 methyladenine glycosylase; DNA-3-methyladenine glycosylase; MDG; Mid1; Mpg; N methylpurine DNA glycosirase; N methylpurine DNA glycosylase; N-methylpurine-DNA glycosylase; PIG11; PIG16; Proliferation inducing protein 11; Proliferation inducing protein 16;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MVTPALQMKKPKQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
92
Rabbit
83
Chicken
58
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29372 As Substrate

Site PTM Type Enzyme
T66 Phosphorylation
T67 Phosphorylation
Y71 Phosphorylation
S73 Phosphorylation
Y75 Phosphorylation
S78 Phosphorylation
K80 Ubiquitination
T115 Phosphorylation
S137 Phosphorylation
K210 Ubiquitination
K229 Ubiquitination
S252 Phosphorylation
Y278 Phosphorylation

Research Backgrounds

Function:

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

Subcellular Location:

Cytoplasm. Mitochondrion matrix>Mitochondrion nucleoid. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds MBD1. Binds SSBP1.

Family&Domains:

Belongs to the DNA glycosylase MPG family.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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