FBP1 Antibody - #DF7325

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Product: FBP1 Antibody
Catalog: DF7325
Description: Rabbit polyclonal antibody to FBP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 37kDa; 37kD(Calculated).
Uniprot: P09467
RRID: AB_2839263

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(100%), Chicken(83%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
FBP1 Antibody detects endogenous levels of total FBP1.
RRID:
AB_2839263
Cite Format: Affinity Biosciences Cat# DF7325, RRID:AB_2839263.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

6-bisphosphatase 1; 6-bisphosphate 1-phosphohydrolase 1; D fructose 1 6 bisphosphate 1 phosphohydrolase 1; D-fructose-1; EC 3.1.3.11; F16P1_HUMAN; FBP; FBP 1; FBP1; FBPase 1; Fructose 1 6 bisphosphatase 1; Fructose bisphosphatase 1; Fructose-1; Growth inhibiting protein 17; Liver fructose bisphosphatase;

Immunogens

Immunogen:
Uniprot:

P09467(F16P1_HUMAN) >>Visit HPA database.

Gene(ID):
FBP1(2203)
Expression:
P09467 F16P1_HUMAN:

Expressed in pancreatic islets.

Description:
Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis.
Sequence:
MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
92
Sheep
92
Rabbit
92
Xenopus
91
Chicken
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09467 As Substrate

Site PTM Type Enzyme
T28 Phosphorylation
Y58 Phosphorylation
S63 Phosphorylation
K72 Acetylation
S88 Phosphorylation
S144 Phosphorylation
Y216 Phosphorylation
Y227 Phosphorylation
K232 Acetylation
Y241 Phosphorylation
Y245 Phosphorylation
T256 Phosphorylation
Y259 Phosphorylation
Y265 Phosphorylation
K269 Acetylation
S271 Phosphorylation
T297 Phosphorylation
T298 Phosphorylation
S321 Phosphorylation
K330 Acetylation

Research Backgrounds

Function:

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.

Tissue Specificity:

Expressed in pancreatic islets.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the FBPase class 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). Fu Brick Tea Manages HFD/STZ-Induced Type 2 Diabetes by Regulating the Gut Microbiota and Activating the IRS1/PI3K/Akt Signaling Pathway. Journal of agricultural and food chemistry, 2022 (PubMed: 35767631) [IF=6.1]
2). FBP1/miR-24-1/enhancer axis activation blocks renal cell carcinoma progression via Warburg effect. Frontiers in Oncology, 2022 (PubMed: 35978816) [IF=4.7]

Application: WB    Species: Human    Sample: renal tissues

Figure 1 FBP1 is reduced in RCC and related to poor survival outcome. (A) Volcano Plot of differentially expressed mRNAs associated with glucose metabolism of Warburg effect in RCC and adjacent normal tissues. Blue represents downregulated genes, and red represents upregulated genes. p < 0.05. (B, C) KEGG pathway and gene oncology (GO) profiling for the downregulated genes in RCC. (D) The mRNA expression levels of FBP1 in RCC and adjacent normal renal tissues from TCGA database by bioinformatic analysis. (E) The mRNA expression levels of FBP1 in RCC and adjacent normal renal tissues detected by qPCR. (F) The protein level of FBP1 in 3 randomly selected paired RCC and adjacent normal renal tissues by western blot. b-actin was used as input control. (G) Representative pictures of immunohistochemical (IHC) staining of 3 randomly selected paired RCC tissue and adjacent normal renal tissues. (H) Kaplan–Meier plots of overall survival in RCC patients with low expression of FBP1 (n=166) compared to high expression of FBP1 (n=178) stratified by the expression levels of FBP1. The cutoff was confirmed as the threshold with the best performance. Log-rank test p value is shown. Results are shown as mean ± S.D., ****p < 0.0001.

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