eEF2K Antibody - #DF7323

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Product: eEF2K Antibody
Catalog: DF7323
Description: Rabbit polyclonal antibody to eEF2K
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 82kDa; 82kD(Calculated).
Uniprot: O00418
RRID: AB_2839261

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
eEF2K Antibody detects endogenous levels of total eEF2K.
RRID:
AB_2839261
Cite Format: Affinity Biosciences Cat# DF7323, RRID:AB_2839261.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C86191; Calcium/calmodulin dependent eukaryotic elongation factor 2; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; Calmodulin dependent protein kinase III; cb365; EC 2.7.11.20; eEF 2 kinase; eEF 2K; eEF-2 kinase; eEF-2K; Eef2k; EEF2K protein; EF2K_HUMAN; Elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; fa04b08; HSU93850; kinase eEF2K; MGC45041; SMEF2K; wu:fa04b08; zgc:100973;

Immunogens

Immunogen:
Uniprot:

O00418(EF2K_HUMAN) >>Visit HPA database.

Gene(ID):
EEF2K(29904)
Description:
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
Sequence:
MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00418 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S18 Phosphorylation
S27 Phosphorylation
S31 Phosphorylation
Y37 Phosphorylation
K58 Ubiquitination
Y59 Phosphorylation
Y60 Phosphorylation
S61 Phosphorylation O00418 (EEF2K)
K65 Ubiquitination
S66 Phosphorylation O00418 (EEF2K)
Y69 Phosphorylation
S70 Phosphorylation
S71 Phosphorylation
S72 Phosphorylation P42345 (MTOR)
S74 Phosphorylation P42345 (MTOR)
S78 Phosphorylation O00418 (EEF2K) , P54646 (PRKAA2) , Q13131 (PRKAA1) , Q96QT4 (TRPM7)
K82 Ubiquitination
K86 Ubiquitination
K93 Ubiquitination
K132 Ubiquitination
S135 Phosphorylation
K151 Ubiquitination
K162 Ubiquitination
K170 Ubiquitination
R178 Methylation
K202 Ubiquitination
K223 Ubiquitination
K235 Ubiquitination
K238 Ubiquitination
S241 Phosphorylation
S243 Phosphorylation
T340 Phosphorylation
K341 Ubiquitination
S345 Phosphorylation
K347 Ubiquitination
T348 Phosphorylation O00418 (EEF2K)
T353 Phosphorylation O00418 (EEF2K)
S359 Phosphorylation P06493 (CDK1) , O15264 (MAPK13)
T364 Phosphorylation
S366 Phosphorylation Q15418 (RPS6KA1) , Q13131 (PRKAA1) , P17612 (PRKACA) , P54646 (PRKAA2) , O00418 (EEF2K) , P23443 (RPS6KB1) , Q9UBS0 (RPS6KB2)
S377 Phosphorylation Q16644 (MAPKAPK3) , P49137 (MAPKAPK2) , Q8IW41 (MAPKAPK5)
S380 Phosphorylation
S392 Phosphorylation
S395 Phosphorylation
S396 Phosphorylation P45984 (MAPK9) , P45983 (MAPK8) , P53778 (MAPK12) , Q15759 (MAPK11) , O15264 (MAPK13) , O00418 (EEF2K) , Q16539 (MAPK14)
S398 Phosphorylation Q13131 (PRKAA1) , P54646 (PRKAA2)
S399 Phosphorylation
T401 Phosphorylation
S416 Phosphorylation
S435 Phosphorylation
S438 Phosphorylation
S441 Phosphorylation O00418 (EEF2K)
Y443 Phosphorylation
S445 Phosphorylation O00418 (EEF2K)
K447 Ubiquitination
S462 Phosphorylation
K467 Sumoylation
Y468 Phosphorylation
S470 Phosphorylation
S474 Phosphorylation O00418 (EEF2K)
S477 Phosphorylation
S478 Phosphorylation
K485 Ubiquitination
S491 Phosphorylation O00418 (EEF2K)
S492 Phosphorylation
S500 Phosphorylation O00418 (EEF2K) , P17612 (PRKACA)
K516 Ubiquitination
K517 Ubiquitination
K520 Ubiquitination
S521 Phosphorylation
K596 Ubiquitination
Y600 Phosphorylation
K603 Ubiquitination
S627 Phosphorylation
K684 Ubiquitination
K705 Ubiquitination
K715 Ubiquitination

PTMs - O00418 As Enzyme

Substrate Site Source
O00418 (EEF2K) S61 Uniprot
O00418 (EEF2K) S66 Uniprot
O00418 (EEF2K) S78 Uniprot
O00418 (EEF2K) T348 Uniprot
O00418 (EEF2K) T353 Uniprot
O00418 (EEF2K) S366 Uniprot
O00418 (EEF2K) S396 Uniprot
O00418 (EEF2K) S441 Uniprot
O00418 (EEF2K) S445 Uniprot
O00418 (EEF2K) S474 Uniprot
O00418 (EEF2K) S491 Uniprot
O00418 (EEF2K) S500 Uniprot
P13639 (EEF2) T54 Uniprot
P13639 (EEF2) T57 Uniprot
P13639 (EEF2) T59 Uniprot

Research Backgrounds

Function:

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

PTMs:

Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.

Subunit Structure:

Monomer or homodimer (Probable). Interacts with Calmodulin/CALM1; this interaction is strictly required for phosphorylation activity.

Family&Domains:

Belongs to the protein kinase superfamily. Alpha-type protein kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

References

1). Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017 (PubMed: 29024627) [IF=3.1]

Application: WB    Species: mouse    Sample:

Fig. 3. Effect of myostatin on translation elongation pathway. C2C12 myotubes (4–5 days post-differentiation) were treated with various concentration recombinant myostatin (0, 0.01, 0.1, 1, 2, 3 µg/ml) for 48 h and then stimulated with 1 µg/mL puromycin for 30 min. Cells were then lysed and cellular extracts were analyzed by Western blot (ABC).

Application: WB    Species: mouse    Sample: C2C12 myotubes

Fig. 3. |Effect of myostatin on translation elongation pathway.C2C12 myotubes (4–5 days post-differentiation) were treated with various concentration recombinant myostatin (0, 0.01, 0.1, 1, 2, 3 µg/ml) for 48 h and then stimulated with 1 µg/mL puromycin for 30 min. Cells were then lysed and cellular extracts were analyzed by Western blot

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