DLD Antibody - #DF7322

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Product: DLD Antibody
Catalog: DF7322
Description: Rabbit polyclonal antibody to DLD
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 54kDa; 54kD(Calculated).
Uniprot: P09622
RRID: AB_2839260

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
DLD Antibody detects endogenous levels of total DLD.
RRID:
AB_2839260
Cite Format: Affinity Biosciences Cat# DF7322, RRID:AB_2839260.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dehydrogenase complex, E3 component; Diaphorase; Dihydrolipoamide dehydrogenase; Dihydrolipoyl dehydrogenase; Dihydrolipoyl dehydrogenase mitochondrial; dld; DLDD; DLDH; DLDH_HUMAN; E3; E3 branched chain aplha-keto acid; E3 component of pyruvate dehydrogenase; E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex; GCSL; Glycine cleavage system L protein; Glycine cleavage system protein L; LAD; lipoamide dehydrogenase; Lipoamide reductase; Lipoyl dehydrogenase; mitochondrial; OTTHUMP00000206744; OTTHUMP00000206746; OTTHUMP00000206748; OTTHUMP00000206749; PHE 3; PHE3; Pyruvate dehydrogenase component E3;

Immunogens

Immunogen:
Uniprot:

P09622(DLDH_HUMAN) >>Visit HPA database.

Gene(ID):
DLD(1738)
Description:
This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.
Sequence:
MQSWSRVYCSLAKRGHFNRISHGLQGLSAVPLRTYADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09622 As Substrate

Site PTM Type Enzyme
K66 Acetylation
K66 Methylation
K72 Acetylation
K104 Acetylation
K104 Ubiquitination
R109 Methylation
K122 Acetylation
K127 Ubiquitination
K132 Ubiquitination
T135 Phosphorylation
K143 Acetylation
K143 Methylation
K143 Ubiquitination
K146 Acetylation
Y153 Phosphorylation
K155 Acetylation
K159 Acetylation
K159 Ubiquitination
K166 Acetylation
K166 Ubiquitination
S230 Phosphorylation
K267 Acetylation
K267 Ubiquitination
K271 Acetylation
K273 Acetylation
T276 Phosphorylation
K277 Acetylation
K277 Ubiquitination
S297 Phosphorylation
K320 Acetylation
K320 Ubiquitination
K346 Acetylation
K346 Ubiquitination
K410 Acetylation
K410 Ubiquitination
K417 Acetylation
K417 Ubiquitination
K420 Acetylation
K420 Ubiquitination
K430 Ubiquitination
T435 Phosphorylation
K440 Ubiquitination
K445 Ubiquitination
S502 Phosphorylation
K505 Methylation

Research Backgrounds

Function:

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity).

PTMs:

Tyrosine phosphorylated.

Subcellular Location:

Mitochondrion matrix. Nucleus. Cell projection>Cilium>Flagellum. Cytoplasmic vesicle>Secretory vesicle>Acrosome.
Note: Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (subunits PDHA (PDHA1 or PDHA2) and PDHB, E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules (by non covalent bonds). The 2-oxoglutarate dehydrogenase complex is composed of OGDH (2-oxoglutarate dehydrogenase; E1), DLST (dihydrolipoamide succinyltransferase; E2) and DLD (dihydrolipoamide dehydrogenase; E3). It contains multiple copies of the three enzymatic components (E1, E2 and E3). In the nucleus, the 2-oxoglutarate dehydrogenase complex associates with KAT2A. Interacts with PDHX.

Family&Domains:

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

References

1). Comprehensive Gene Analysis Reveals Cuproptosis-Related Gene Signature Associated with M2 Macrophage in Staphylococcus aureus-Infected Osteomyelitis. Journal of inflammation research, 2024 (PubMed: 38770176) [IF=4.5]

Application: IHC    Species: Rat    Sample:

Figure 11 Validation for 3 M2R-CRGs through independent dataset (GSE30119), RT-qPCR, and immunohistochemistry. In the independent dataset, SLC31A1 (A), DLD (B), and MTF1 (C) were all significantly upregulated in the osteomyelitis rat group. Total RNA and protein were extracted from both the osteomyelitis rat group and the sham operation group. The mRNA expression levels of SLC31A1 (D), DLD (E), and MTF1 (F) were significantly increased in the osteomyelitis rat group. Furthermore, the protein levels of SLC31A1 (G), DLD (H), and MTF1 (I) showed a significant upregulation in the osteomyelitis rat group. Significance: *: P

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