CFP Antibody - #DF7320

(4)  
Product: CFP Antibody
Catalog: DF7320
Description: Rabbit polyclonal antibody to CFP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse
Mol.Wt.: 51kDa; 51kD(Calculated).
Uniprot: P27918
RRID: AB_2839258

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(82%)
Clonality:
Polyclonal
Specificity:
CFP Antibody detects endogenous levels of total CFP.
RRID:
AB_2839258
Cite Format: Affinity Biosciences Cat# DF7320, RRID:AB_2839258.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BFD; CFP; Complement factor P; Complement factor properdin; Factor P; PFC; PFD; PROP_HUMAN; Properdin; Properdin P factor complement;

Immunogens

Immunogen:
Uniprot:

P27918(PROP_HUMAN) >>Visit HPA database.

Gene(ID):
CFP(5199)
Description:
This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Sequence:
MITEGAQAPRLLLPPLLLLLTLPATGSDPVLCFTQYEESSGKCKGLLGGGVSVEDCCLNTAFAYQKRSGGLCQPCRSPRWSLWSTWAPCSVTCSEGSQLRYRRCVGWNGQCSGKVAPGTLEWQLQACEDQQCCPEMGGWSGWGPWEPCSVTCSKGTRTRRRACNHPAPKCGGHCPGQAQESEACDTQQVCPTHGAWATWGPWTPCSASCHGGPHEPKETRSRKCSAPEPSQKPPGKPCPGLAYEQRRCTGLPPCPVAGGWGPWGPVSPCPVTCGLGQTMEQRTCNHPVPQHGGPFCAGDATRTHICNTAVPCPVDGEWDSWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRCAGQQQDIRHCYSIQHCPLKGSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSMVEGQGEKNVTFWGRPLPRCEELQGQKLVVEEKRPCLHVPACKDPEEEEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
82
Pig
73
Bovine
73
Sheep
73
Dog
73
Rabbit
73
Xenopus
50
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P27918 As Substrate

Site PTM Type Enzyme
S39 Phosphorylation
S40 Phosphorylation
W83 C-Glycosylation
W86 C-Glycosylation
T92 O-Glycosylation
W139 C-Glycosylation
W142 C-Glycosylation
W145 C-Glycosylation
T151 O-Glycosylation
W196 C-Glycosylation
W199 C-Glycosylation
S208 O-Glycosylation
W260 C-Glycosylation
W263 C-Glycosylation
T272 O-Glycosylation
W321 C-Glycosylation
W324 C-Glycosylation
W382 C-Glycosylation
W385 C-Glycosylation
W388 C-Glycosylation
N428 N-Glycosylation

Research Backgrounds

Function:

A positive regulator of the alternate pathway (AP) of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Inhibits CFI-CFH mediated degradation of Complement C3 beta chain (C3b).

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

In plasma, properdin exists as dimers, trimers or tetramers in the relative proportions of 26:54:20. Interacts with the pro-C3-convertase enzyme complex (C3b-Bb) comprised of Complement C3 beta chain (C3b) and the Complement factor B Bb fragment (Bb), where it binds (via its TSP type-1 5 domain) with C3b and Bb. This interaction stabilizes the complex and allows it to become the active C3-convertase enzyme complex (C3b-Bb-FP). Interacts with C3b. Interacts with CFB.

Family&Domains:

TSP type-1 domain 0 binds to TSP type-1 domain 4, and TSP type-1 domain 1 binds to TSP type-1 domain 6 (PubMed:15491616, PubMed:28264884, PubMed:31507604). These interactions mediate multimerization (PubMed:15491616, PubMed:28264884, PubMed:31507604).

Research Fields

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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