WIF1 Antibody - #DF7317
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| Product: | WIF1 Antibody |
| Catalog: | DF7317 |
| Description: | Rabbit polyclonal antibody to WIF1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 41kDa; 42kD(Calculated). |
| Uniprot: | Q9Y5W5 |
| RRID: | AB_2839255 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(82%), Horse(91%), Sheep(82%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
WIF1 Antibody detects endogenous levels of total WIF1.
RRID:
AB_2839255
Cite Format: Affinity Biosciences Cat# DF7317, RRID:AB_2839255.
Cite Format: Affinity Biosciences Cat# DF7317, RRID:AB_2839255.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
WIF-1; wif1; WIF1_HUMAN; Wnt inhibitory factor 1;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
he protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Sequence:
- Q9Y5W5 WIF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARRSAFPAAALWLWSILLCLLALRAEAGPPQEESLYLWIDAHQARVLIGFEEDILIVSEGKMAPFTHDFRKAQQRMPAIPVNIHSMNFTWQAAGQAEYFYEFLSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCNERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCRNGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPPESNYIW
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Dog
100
Chicken
100
Rabbit
100
Horse
91
Xenopus
91
Bovine
82
Sheep
82
Zebrafish
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y5W5 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T215 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Subcellular Location:
Secreted.
Subunit Structure:
Interacts with MYOC.
Research Fields
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
References
1). Upregulated miR-374a-5p drives psoriasis pathogenesis through WIF1 downregulation and Wnt5a/NF-κB activation. Cellular signalling, 2024
(PubMed: 38604345)
[IF=4.8]
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