RANGAP1 Antibody - #DF7315
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF7315, RRID:AB_2839253.
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Fug 1; Fug1; GTPase-activating protein, RAN, 1; KIAA1835; MGC20266; OTTHUMP00000028918; OTTHUMP00000198755; OTTHUMP00000198756; OTTHUMP00000198757; OTTHUMP00000198758; RAGP1_HUMAN; Ran 1; RAN GTPase activating protein 1; Ran GTPase-activating protein 1; Ran1; RANGAP 1; RANGAP; RanGAP1; SD; Segregation distorter homolog; Segregation distortion;
Immunogens
- P46060 RAGP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASEDIAKLAETLAKTQVAGGQLSFKGKSLKLNTAEDAKDVIKEIEDFDSLEALRLEGNTVGVEAARVIAKALEKKSELKRCHWSDMFTGRLRTEIPPALISLGEGLITAGAQLVELDLSDNAFGPDGVQGFEALLKSSACFTLQELKLNNCGMGIGGGKILAAALTECHRKSSAQGKPLALKVFVAGRNRLENDGATALAEAFRVIGTLEEVHMPQNGINHPGITALAQAFAVNPLLRVINLNDNTFTEKGAVAMAETLKTLRQVEVINFGDCLVRSKGAVAIADAIRGGLPKLKELNLSFCEIKRDAALAVAEAMADKAELEKLDLNGNTLGEEGCEQLQEVLEGFNMAKVLASLSDDEDEEEEEEGEEEEEEAEEEEEEDEEEEEEEEEEEEEEPQQRGQGEKSATPSRKILDPNTGEPAPVLSSPPPADVSTFLAFPSPEKLLRLGPKSSVLIAQQTDTSDPEKVVSAFLKVSSVFKDEATVRMAVQDAVDALMQKAFNSSSFNSNTFLTRLLVHMGLLKSEDKVKAIANLYGPLMALNHMVQQDYFPKALAPLLLAFVTKPNSALESCSFARHSLLQTLYKV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P46060 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| K8 | Sumoylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| K26 | Sumoylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K28 | Sumoylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| R81 | Methylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| C169 | S-Nitrosylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| T247 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| R289 | Methylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| S358 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K406 | Methylation | Uniprot | |
| T409 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K413 | Acetylation | Uniprot | |
| T419 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S435 | Phosphorylation | Uniprot | |
| T436 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K452 | Sumoylation | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| S453 | Phosphorylation | Uniprot | |
| T461 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| T485 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| K524 | Acetylation | Uniprot | |
| K524 | Sumoylation | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| K528 | Sumoylation | Uniprot | |
| K530 | Sumoylation | Uniprot | |
| Y585 | Phosphorylation | Uniprot |
Research Backgrounds
GTPase activator for RAN. Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export. Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export.
Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBE2I.
Cytoplasm. Nucleus>Nucleoplasm. Nucleus envelope. Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Spindle.
Note: Cytoplasmic during interphase. Detected at the nuclear envelope during interphase (PubMed:11854305, PubMed:15037602). Targeted to the nuclear pores after sumoylation (PubMed:11854305). During mitosis, associates with mitotic spindles, but is essentially not detected at the spindle poles (PubMed:11854305, PubMed:15037602). Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase (PubMed:11854305). Mitotic location also requires sumoylation (PubMed:11854305).
Highly expressed in brain, thymus and testis.
Homodimer. Interacts with RAN. Forms a complex with RANBP2/NUP358, NXF1 and NXT1. Forms a tight complex in association with RANBP2/NUP358 and UBE2I/UBC9, the ubiquitin-conjugating enzyme E2. Interacts with UBE2I; the interaction conjugates SUMO1 to RANGAP1, and subsequently stabilizes interactions of sumoylated RANGAP1 with RANBP2/NUP358. The complex composed of RANBP2, SUMO1, RANGAP1 and UBE2I associates with nuclear pore complexes. Identified in a complex composed of RAN, RANBP2, sumoylated RANGAP1, UBE2I and XPO1. Identified in a complex composed of RAN, RANGAP1 and RANBP1. Interacts with TRAF6. Interacts with SUMO1 and SENP1. Interacts (when sumoylated) with MYCBP2; interaction inhibits MYCBP2 E3 ubiquitin-protein ligase activity and promotes MYCBP2 translocation to the nucleus.
Belongs to the RNA1 family.
Research Fields
· Genetic Information Processing > Translation > RNA transport.
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