PSMC6 Antibody | Affinity Biosciences

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Western blot analysis of 293T whole cell lysates, using PSMC6 Antibody.

DF7312 at 1/100 staining Mouse liver tissue by IHC-P.

DF7312 at 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P.

Product:	PSMC6 Antibody
Catalog:	DF7312
Description:	Rabbit polyclonal antibody to PSMC6
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	44kDa; 44kD(Calculated).
Uniprot:	P62333
RRID:	AB_2839250

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

PSMC6 Antibody detects endogenous levels of total PSMC6.

RRID:

AB_2839250
Cite Format: Affinity Biosciences Cat# DF7312, RRID:AB_2839250.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

26S protease regulatory subunit 10B; 26S protease regulatory subunit S10B; 26S proteasome AAA-ATPase subunit RPT4; CADP44; P44; Proteasome 26S subunit ATPase 6; Proteasome subunit p42; PRS10_HUMAN; PSMC6; Rpt4; SUG2;

Immunogens

Immunogen:

Uniprot:

P62333(PRS10_HUMAN) >>Visit HPA database.

Gene(ID):

PSMC6(5706)

Description:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12.

Sequence:

P62333 PRS10_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADPRDKALQDYRKKLLEHKEIDGRLKELREQLKELTKQYEKSENDLKALQSVGQIVGEVLKQLTEEKFIVKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSEQIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARAVASQLDCNFLKVVSSSIVDKYIGESARLIREMFNYARDHQPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDTLHRVKMIMATNRPDTLDPALLRPGRLDRKIHIDLPNEQARLDILKIHAGPITKHGEIDYEAIVKLSDGFNGADLRNVCTEAGMFAIRADHDFVVQEDFMKAVRKVADSKKLESKLDYKPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Dog

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62333 As Substrate

P62333

Site	PTM Type	Source
K7	Ubiquitination	Uniprot
K15	Acetylation	Uniprot
K15	Ubiquitination	Uniprot
K20	Acetylation	Uniprot
K20	Ubiquitination	Uniprot
K27	Ubiquitination	Uniprot
K34	Ubiquitination	Uniprot
K38	Ubiquitination	Uniprot
K42	Ubiquitination	Uniprot
K48	Ubiquitination	Uniprot
S52	Phosphorylation	Uniprot
K62	Ubiquitination	Uniprot
K68	Acetylation	Uniprot
K68	Ubiquitination	Uniprot
K72	Acetylation	Uniprot
K72	Ubiquitination	Uniprot
K91	Ubiquitination	Uniprot
T103	Phosphorylation	Uniprot
T104	Phosphorylation	Uniprot
T106	Phosphorylation	Uniprot
S131	Phosphorylation	Uniprot
Y132	Phosphorylation	Uniprot
K168	Ubiquitination	Uniprot
Y173	Phosphorylation	Uniprot
K180	Ubiquitination	Uniprot
C193	S-Nitrosylation	Uniprot
K197	Ubiquitination	Uniprot
S201	Phosphorylation	Uniprot
S202	Phosphorylation	Uniprot
K206	Acetylation	Uniprot
K206	Ubiquitination	Uniprot
Y207	Phosphorylation	Uniprot
Y221	Phosphorylation	Uniprot
S244	Phosphorylation	Uniprot
K274	Ubiquitination	Uniprot
K298	Ubiquitination	Uniprot
K314	Ubiquitination	Uniprot
K322	Ubiquitination	Uniprot
Y328	Phosphorylation	Uniprot
K333	Ubiquitination	Uniprot
K369	Ubiquitination	Uniprot
K383	Ubiquitination	Uniprot
Y386	Phosphorylation	Uniprot

Research Backgrounds

P62333_PRS10_HUMAN

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC6 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

Subcellular Location:

Cytoplasm. Nucleus.

Subunit Structure:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC6 and few additional components. Interacts with PAAF1.

Family&Domains:

Belongs to the AAA ATPase family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PSMC6 Antibody - #DF7312