PA2G4 Antibody - #DF7311

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Product: PA2G4 Antibody
Catalog: DF7311
Description: Rabbit polyclonal antibody to PA2G4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 43kDa; 44kD(Calculated).
Uniprot: Q9UQ80
RRID: AB_2839249

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PA2G4 Antibody detects endogenous levels of total PA2G4.
RRID:
AB_2839249
Cite Format: Affinity Biosciences Cat# DF7311, RRID:AB_2839249.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

38kDa; AA672939; Cell cycle protein p38 2G4 homolog; Cell cycle protein p38-2G4 homolog; ErbB-3 binding protein 1; ErbB3 binding protein 1; ErbB3-binding protein 1; ErbB3-binding protein Ebp1; hG4 1; hG4-1; IRES-specific cellular trans-acting factor 45 kDa; MGC81621; MGC94070; Mpp1; p38 2G4; Pa2g4; PA2G4_HUMAN; Plfap; Proliferation associated 2G4; Proliferation associated 2G4, 38-KD; Proliferation-associated 2G4, 38kDa; Proliferation-associated 2G4, a; Proliferation-associated protein 1; Proliferation-associated protein 2G4; Protein p38-2G4; si:dz150i12.2; wu:fb19b11; wu:ft56d05; zgc:86732;

Immunogens

Immunogen:
Uniprot:

Q9UQ80(PA2G4_HUMAN) >>Visit HPA database.

Gene(ID):
PA2G4(5036)
Expression:
Q9UQ80 PA2G4_HUMAN:

Isoform 2 is undetectable whereas isoform 1 is strongly expressed in cancer cells (at protein level). Isoform 1 and isoform 2 are widely expressed, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta and liver.

Description:
This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified.
Sequence:
MSGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
92
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UQ80 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T11 Phosphorylation
K20 Ubiquitination
K22 Ubiquitination
R30 Methylation
S34 Phosphorylation
S44 Phosphorylation
T60 Phosphorylation
K62 Ubiquitination
K72 Ubiquitination
S81 Phosphorylation
S90 Phosphorylation
K93 Sumoylation
K93 Ubiquitination
S94 Phosphorylation
Y98 Phosphorylation
K101 Ubiquitination
K144 Ubiquitination
K158 Ubiquitination
K172 Methylation
K172 Ubiquitination
K191 Ubiquitination
K199 Methylation
K199 Ubiquitination
T206 Phosphorylation
K210 Ubiquitination
K238 Ubiquitination
K248 Ubiquitination
K253 Ubiquitination
Y255 Phosphorylation
K258 Ubiquitination
K260 Methylation
T261 Phosphorylation Q13153 (PAK1)
S267 Phosphorylation
R271 Methylation
R281 Methylation
K287 Acetylation
K288 Ubiquitination
K298 Acetylation
K298 Sumoylation
K298 Ubiquitination
K311 Ubiquitination
S335 Phosphorylation
K344 Sumoylation
K344 Ubiquitination
S345 Phosphorylation
K355 Ubiquitination
S360 Phosphorylation P17252 (PRKCA) , Q05655 (PRKCD)
S361 Phosphorylation
S363 Phosphorylation P05129 (PRKCG) , P17252 (PRKCA) , P05771 (PRKCB)
T366 Phosphorylation P05771 (PRKCB) , P17252 (PRKCA) , P05129 (PRKCG)
K373 Ubiquitination
S375 Phosphorylation
K376 Ubiquitination
T377 Phosphorylation
T382 Phosphorylation
S383 Phosphorylation
T386 Phosphorylation

Research Backgrounds

Function:

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).

PTMs:

Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.

In cancer cells, isoform 2 is polyubiquitinated leading to its proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.

Subcellular Location:

Cytoplasm. Nucleus>Nucleolus.
Note: Translocates to the nucleus upon treatment with HRG. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 2 is undetectable whereas isoform 1 is strongly expressed in cancer cells (at protein level). Isoform 1 and isoform 2 are widely expressed, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta and liver.

Subunit Structure:

Isoform 2 interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with NCL/nucleolin. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation. Interacts with AKT1 (By similarity). Isoform 1 and isoform 2 interact with RNF20. Isoform 2 interacts with HUWE1. Interacts with DNAJC21.

Family&Domains:

Belongs to the peptidase M24 family.

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