DBI Antibody | Affinity Biosciences

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Western blot analysis of extracts from hela , using DBI antibody.

Product:	DBI Antibody
Catalog:	DF7307
Description:	Rabbit polyclonal antibody to DBI
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	10kDa; 10kD(Calculated).
Uniprot:	P07108
RRID:	AB_2839245

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(92%), Bovine(92%), Horse(83%), Sheep(92%), Rabbit(83%), Chicken(83%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

DBI Antibody detects endogenous levels of total DBI.

RRID:

AB_2839245
Cite Format: Affinity Biosciences Cat# DF7307, RRID:AB_2839245.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACBD 1; ACBD1; ACBP; ACBP_HUMAN; Acyl CoA binding domain containing 1; Acyl CoA binding protein; Acyl Coenzyme A binding domain containing 1; Acyl coenzyme A binding protein; Acyl-CoA-binding protein; CCK RP; CCKRP; Cholecystokinin releasing peptide trypsin sensitive; DBI; Diazepam-binding inhibitor; Endozepine; EP; GABA receptor modulator; MGC70414;

Immunogens

Immunogen:

Uniprot:

P07108(ACBP_HUMAN) >>Visit HPA database.

Gene(ID):

DBI(1622)

Expression:

P07108 ACBP_HUMAN:

Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.

Description:

This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.

Sequence:

P07108 ACBP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSQAEFEKAAEEVRHLKTKPSDEEMLFIYGHYKQATVGDINTERPGMLDFTGKAKWDAWNELKGTSKEDAMKAYINKVEELKKKYGI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Zebrafish

Horse

Xenopus

Chicken

Rabbit

Pig

Dog

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07108 As Substrate

P07108

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
S2	Phosphorylation		Uniprot
K8	Acetylation		Uniprot
K17	Acetylation		Uniprot
K19	Acetylation		Uniprot
Y29	Phosphorylation		Uniprot
Y32	Phosphorylation		Uniprot
T42	Phosphorylation	Q05655 (PRKCD)	Uniprot
T51	Phosphorylation		Uniprot
K53	Ubiquitination		Uniprot
K55	Acetylation		Uniprot
K55	Ubiquitination		Uniprot
K63	Ubiquitination		Uniprot
T65	Phosphorylation		Uniprot
S66	Phosphorylation		Uniprot
K77	Acetylation		Uniprot
K77	Ubiquitination		Uniprot
K82	Ubiquitination		Uniprot

Research Backgrounds

P07108_ACBP_HUMAN

Function:

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

Subcellular Location:

Endoplasmic reticulum. Golgi apparatus.
Note: Golgi localization is dependent on ligand binding (PubMed:17953517).

Tissue Specificity:

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the ACBP family.

Research Fields

· Organismal Systems > Endocrine system > PPAR signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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DBI Antibody - #DF7307