SMARCE1 Antibody - #DF7301

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Product: SMARCE1 Antibody
Catalog: DF7301
Description: Rabbit polyclonal antibody to SMARCE1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 46kDa; 47kD(Calculated).
Uniprot: Q969G3
RRID: AB_2839239

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Horse(100%), Sheep(90%), Rabbit(100%), Dog(90%)
Clonality:
Polyclonal
Specificity:
SMARCE1 Antibody detects endogenous levels of total SMARCE1.
RRID:
AB_2839239
Cite Format: Affinity Biosciences Cat# DF7301, RRID:AB_2839239.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BAF57; BRG1 associated factor 57; BRG1-associated factor 57; Chromatin remodeling complex BRG1 associated factor 57; FLJ35648; SMARCE 1; SMARCE1; SMCE1_HUMAN; SWI/SNF related matrix associated actin dependent regulator of chromatin e1; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1;

Immunogens

Immunogen:
Uniprot:

Q969G3(SMCE1_HUMAN) >>Visit HPA database.

Gene(ID):
SMARCE1(6605)
Description:
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
Sequence:
MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYLAYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEVDMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEKKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Pig
90
Sheep
90
Dog
90
Bovine
70
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969G3 As Substrate

Site PTM Type Enzyme
K3 Acetylation
K3 Methylation
R4 Methylation
S6 Phosphorylation
Y7 Phosphorylation
T12 Phosphorylation
T17 Phosphorylation
S21 Phosphorylation
T22 Phosphorylation
Y28 Phosphorylation
Y31 Phosphorylation
S32 Phosphorylation
R40 Methylation
T47 Phosphorylation
T58 Phosphorylation
Y73 Phosphorylation
K85 Ubiquitination
K92 Sumoylation
K92 Ubiquitination
K98 Ubiquitination
K112 Ubiquitination
Y115 Phosphorylation
K131 Ubiquitination
S136 Phosphorylation
Y139 Phosphorylation
Y142 Phosphorylation
K146 Ubiquitination
S157 Phosphorylation
K166 Ubiquitination
Y170 Phosphorylation
K189 Ubiquitination
T191 Phosphorylation
T193 Phosphorylation
S204 Phosphorylation
K228 Methylation
K240 Ubiquitination
K258 Ubiquitination
T263 Phosphorylation
S265 Phosphorylation
K271 Ubiquitination
K277 Sumoylation
K277 Ubiquitination
K284 Ubiquitination
S314 Phosphorylation
S317 Phosphorylation
K328 Acetylation
K328 Ubiquitination
T350 Phosphorylation
S353 Phosphorylation
S362 Phosphorylation
T363 Phosphorylation

Research Backgrounds

Function:

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells.

PTMs:

Ubiquitinated by TRIP12, leading to its degradation by the proteasome. Ubiquitination is prevented upon interaction between TRIP12 and SMARCC1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the multiprotein chromatin-remodeling complexes SWI/SNF: SWI/SNF-A (BAF), SWI/SNF-B (PBAF) and related complexes. The canonical complex contains a catalytic subunit (either SMARCA4/BRG1/BAF190A or SMARCA2/BRM/BAF190B), and at least SMARCE1, ACTL6A/BAF53, SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1/SNF5/BAF47. Other subunits specific to each of the complexes may also be present permitting several possible combinations developmentally and tissue specific. Component of the BAF complex, which includes at least actin (ACTB), ARID1A/BAF250A, ARID1B/BAF250B, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin. May be a component of the SWI/SNF-B (PBAF) chromatin remodeling complex, at least composed of SMARCA4/BRG1, SMARCB1/BAF47/SNF5, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C, SMARCC1/BAF155, SMARCC2/BAF170, PBRM1/BAF180, ARID2/BAF200 and actin (ACTB). Interacts with BRDT (By similarity). Also binds to the SRC/p160 family of histone acetyltransferases (HATs) composed of NCOA1, NCOA2, and NCOA3. Interacts with RCOR1/CoREST, NR3C1 and ZMIM2/ZIMP7.

Family&Domains:

The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development.

Research Fields

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

References

1). High expression of SMARCE1 predicts poor prognosis and promotes cell growth and metastasis in gastric cancer. Cancer Management and Research, 2019 (PubMed: 31118775) [IF=3.3]

Application: WB    Species: human    Sample: T and NT tissues

Figure 1 |SMARCE1 is upregulated in GC tissues.(A and B) the mRNA expression of SMARCE1 was significantly higher in GC tissues than in normal tissues according to TCGA data and Oncomine dataset . (C) The mRNA expression of SMARCE1 was measured in 20 paired fresh human GC tissues (T) and matched adjacent nontumor tissues (NT). (D) The protein expression of SMARCE1 in eight randomly selected paired T and NT tissues was examined using western blotting. β-actin was used as a control.

Application: IHC    Species: human    Sample: 122 paired NT and human GC samples

Figure 2 |Elevated SMARCE1 expression indicates poor prognosis in GC patients.(A) Representative image of SMARCE1 immunohistochemical staining in 122 paired NT and human GC samples. Up: magnification ×100; down: magnification x400

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