PSME1 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from various samples, using PSME1 Antibody.

DF7298 at 1/100 staining Rat brain tissue by IHC-P.

Product:	PSME1 Antibody
Catalog:	DF7298
Description:	Rabbit polyclonal antibody to PSME1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit
Mol.Wt.:	28kDa; 29kD(Calculated).
Uniprot:	Q06323
RRID:	AB_2839237

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

PSME1 Antibody detects endogenous levels of total PSME1.

RRID:

AB_2839237
Cite Format: Affinity Biosciences Cat# DF7298, RRID:AB_2839237.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

11S regulator complex alpha subunit; 11S regulator complex subunit alpha; 29 kD MCP activator subunit; 29kD MCP activator subunit; Activator of multicatalytic protease subunit 1; IFI5111; IGUP I-5111; Interferon gamma IEF SSP 5111; Interferon gamma inducible protein 5111; Interferon gamma up regulated I 5111 protein; Interferon gamma up-regulated I-5111 protein; MGC8628; PA28a; PA28alpha; Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); Proteasome activator 28 subunit alpha; Proteasome activator complex subunit 1; Proteasome activator subunit 1; PSME1; PSME1_HUMAN; REG-alpha; REGalpha;

Immunogens

Immunogen:

Uniprot:

Q06323(PSME1_HUMAN) >>Visit HPA database.

Gene(ID):

PSME1(5720)

Description:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants.

Sequence:

Q06323 PSME1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Rabbit

100

Zebrafish

Xenopus

Horse

Dog

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q06323 As Substrate

Q06323

Site	PTM Type	Source
K13	Acetylation	Uniprot
K13	Ubiquitination	Uniprot
T23	Phosphorylation	Uniprot
K24	Ubiquitination	Uniprot
T25	Phosphorylation	Uniprot
S31	Phosphorylation	Uniprot
K35	Acetylation	Uniprot
K35	Ubiquitination	Uniprot
K36	Ubiquitination	Uniprot
S38	Phosphorylation	Uniprot
K45	Ubiquitination	Uniprot
S55	Phosphorylation	Uniprot
K58	Sumoylation	Uniprot
K58	Ubiquitination	Uniprot
K90	Acetylation	Uniprot
K109	Ubiquitination	Uniprot
K155	Ubiquitination	Uniprot
S175	Phosphorylation	Uniprot
K176	Ubiquitination	Uniprot
K187	Ubiquitination	Uniprot
K190	Ubiquitination	Uniprot
Y209	Phosphorylation	Uniprot
R213	Methylation	Uniprot
R220	Methylation	Uniprot
K232	Acetylation	Uniprot
K245	Ubiquitination	Uniprot
Y249	Phosphorylation	Uniprot

Research Backgrounds

Q06323_PSME1_HUMAN

Function:

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

Subunit Structure:

Heterodimer of PSME1 and PSME2, which forms a hexameric ring. PSME1 can form homoheptamers.

Family&Domains:

Belongs to the PA28 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PSME1 Antibody - #DF7298