Lumican Antibody - #DF7293
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| Product: | Lumican Antibody |
| Catalog: | DF7293 |
| Description: | Rabbit polyclonal antibody to Lumican |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken |
| Mol.Wt.: | 38kDa; 38kD(Calculated). |
| Uniprot: | P51884 |
| RRID: | AB_2839232 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Lumican Antibody detects endogenous levels of total Lumican.
RRID:
AB_2839232
Cite Format: Affinity Biosciences Cat# DF7293, RRID:AB_2839232.
Cite Format: Affinity Biosciences Cat# DF7293, RRID:AB_2839232.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Keratan sulfate proteoglycan lumican; KSPG lumican; LDC; LUM; LUM_HUMAN; Lumican; Lumican precursor; SLRR2D;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Description:
This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair.
Sequence:
- P51884 LUM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLSAFTLFLALIGGTSGQYYDYDFPLSIYGQSSPNCAPECNCPESYPSAMYCDELKLKSVPMVPPGIKYLYLRNNQIDHIDEKAFENVTDLQWLILDHNLLENSKIKGRVFSKLKQLKKLHINHNNLTESVGPLPKSLEDLQLTHNKITKLGSFEGLVNLTFIHLQHNRLKEDAVSAAFKGLKSLEYLDLSFNQIARLPSGLPVSLLTLYLDNNKISNIPDEYFKRFNALQYLRLSHNELADSGIPGNSFNVSSLVELDLSYNKLKNIPTVNENLENYYLEVNQLEKFDIKSFCKILGPLSYSKIKHLRLDGNRISETSLPPDMYECLRVANEVTLN
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Zebrafish
90
Rabbit
71
Xenopus
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51884 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N88 | N-Glycosylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| N160 | N-Glycosylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K181 | Acetylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| T209 | Phosphorylation | Uniprot | |
| Y211 | Phosphorylation | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| K226 | Acetylation | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot |
Research Backgrounds
PTMs:
Sulfated on tyrosine residue(s).
Subcellular Location:
Secreted>Extracellular space>Extracellular matrix.
Tissue Specificity:
Cornea and other tissues.
Subunit Structure:
Binds to laminin.
Family&Domains:
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Research Fields
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
References
1). Stiffness-dependent dynamic effect of inflammation on keratocyte phenotype and differentiation. Biomedical Materials, 2023
(PubMed: 37068490)
[IF=4.0]
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