IMPDH2 Antibody - #DF7291

(2)  
Product: IMPDH2 Antibody
Catalog: DF7291
Description: Rabbit polyclonal antibody to IMPDH2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Dog
Mol.Wt.: 55kDa; 56kD(Calculated).
Uniprot: P12268
RRID: AB_2839230

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Horse(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
IMPDH2 Antibody detects endogenous levels of total IMPDH2.
RRID:
AB_2839230
Cite Format: Affinity Biosciences Cat# DF7291, RRID:AB_2839230.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

IMDH2_HUMAN; IMP (inosine monophosphate) dehydrogenase 2; IMP dehydrogenase 2; IMP oxireductase 2; IMPD 2; IMPD2; IMPDH 2; IMPDH II; IMPDH-II; Impdh2; Inosine 5' monophosphate dehydrogenase 2; Inosine 5' phosphate dehydrogenase 2; Inosine monophosphate dehydrogenase type II; Inosine-5''-monophosphate dehydrogenase 2;

Immunogens

Immunogen:
Uniprot:

P12268(IMDH2_HUMAN) >>Visit HPA database.

Gene(ID):
IMPDH2(3615)
Expression:
P12268 IMDH2_HUMAN:

IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.

Description:
This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.
Sequence:
MADYLISGGTSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYIDFTADQVDLTSALTKKITLKTPLVSSPMDTVTEAGMAIAMALTGGIGFIHHNCTPEFQANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMGSGSICITQEVLACGRPQATAVYKVSEYARRFGVPVIADGGIQNVGHIAKALALGASTVMMGSLLAATTEAPGEYFFSDGIRLKKYRGMGSLDAMDKHLSSQNRYFSEADKIKVAQGVSGAVQDKGSIHKFVPYLIAGIQHSCQDIGAKSLTQVRAMMYSGELKFEKRTSSAQVEGGVHSLHSYEKRLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Dog
88
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12268 As Substrate

Site PTM Type Enzyme
K109 Ubiquitination
S122 Phosphorylation P24941 (CDK2)
K124 Ubiquitination
K134 Acetylation
K134 Ubiquitination
C140 S-Nitrosylation
S159 Phosphorylation
S160 Phosphorylation
C173 S-Nitrosylation
K181 Ubiquitination
K195 Acetylation
K195 Ubiquitination
R203 Methylation
K205 Ubiquitination
K206 Ubiquitination
K208 Acetylation
K208 Ubiquitination
R231 Methylation
S237 Phosphorylation
K238 Ubiquitination
K242 Ubiquitination
K257 Ubiquitination
K288 Ubiquitination
K291 Ubiquitination
K293 Acetylation
K293 Ubiquitination
K311 Ubiquitination
T345 Phosphorylation
Y348 Phosphorylation
K349 Ubiquitination
K375 Ubiquitination
Y400 Phosphorylation
Y411 Phosphorylation
R412 Methylation
S416 Phosphorylation
K422 Acetylation
K422 Ubiquitination
S425 Phosphorylation
S426 Phosphorylation
R429 Methylation
Y430 Phosphorylation
S432 Phosphorylation
K436 Acetylation
K436 Ubiquitination
K438 Ubiquitination
S444 Phosphorylation
K450 Ubiquitination
K455 Ubiquitination
Y459 Phosphorylation
K474 Ubiquitination
K489 Ubiquitination
S505 Phosphorylation
Y509 Phosphorylation
K511 Acetylation
K511 Ubiquitination

Research Backgrounds

Function:

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.

PTMs:

The N-terminus is blocked.

Acetylated by CLOCK in a circadian manner.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.

Subunit Structure:

Homotetramer Ref.25, Ref.26). Interacts with CLOCK; in a circadian manner.

Family&Domains:

Belongs to the IMPDH/GMPR family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

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