BPI Antibody - #DF7282
| Product: | BPI Antibody |
| Catalog: | DF7282 |
| Description: | Rabbit polyclonal antibody to BPI |
| Application: | WB IHC |
| Reactivity: | Human, Rat |
| Mol.Wt.: | 54kDa; 54kD(Calculated). |
| Uniprot: | P17213 |
| RRID: | AB_2839221 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF7282, RRID:AB_2839221.
Fold/Unfold
Bactericidal permeability-increasing protein; bactericidal/permeability-increasing protein; BPI; BPI fold containing family D, member 1; BPIFD1; CAP 57; rBPI; recombinant BPI holoprotein, rBPI;
Immunogens
- P17213 BPI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRENMARGPCNAPRWASLMVLVAIGTAVTAAVNPGVVVRISQKGLDYASQQGTAALQKELKRIKIPDYSDSFKIKHLGKGHYSFYSMDIREFQLPSSQISMVPNVGLKFSISNANIKISGKWKAQKRFLKMSGNFDLSIEGMSISADLKLGSNPTSGKPTITCSSCSSHINSVHVHISKSKVGWLIQLFHKKIESALRNKMNSQVCEKVTNSVSSELQPYFQTLPVMTKIDSVAGINYGLVAPPATTAETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVYK
PTMs - P17213 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K327 | Acetylation | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| K454 | Ubiquitination | Uniprot |
Research Backgrounds
The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
Secreted. Cytoplasmic granule membrane.
Note: Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.
Restricted to cells of the myeloid series.
Monomer. Homodimer; disulfide-linked.
The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N- and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested.
The N- and C-terminal barrels adopt an identical fold despite having only 13% of conserved residues.
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
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