Product: SLC4A4 Antibody
Catalog: DF7280
Source: Rabbit
Application: WB, IHC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 121kD; 121kD(Calculated).
Uniprot: Q9Y6R1
RRID: AB_2839219

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
SLC4A4 Antibody detects endogenous levels of total SLC4A4.
RRID:
AB_2839219
Cite Format: Affinity Biosciences Cat# DF7280, RRID:AB_2839219.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp781H1314; Electrogenic sodium bicarbonate cotransporter 1; hhNMC; HNBC 1; HNBC1; kNBC 1; KNBC; kNBC1; Na(+)/HCO3(-) cotransporter; Na+HCO3- cotransporter 4; NBC 1; NBC 2; NBC1; NBC2; Nbc4; NBCE 1; NBCE1; OTTHUMP00000160355; OTTHUMP00000218884; OTTHUMP00000218885; PNBC; S4A4_HUMAN; SLC4A4; SLC4A5; Sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter, kidney; sodium bicarbonate cotransporter, pancreas); Sodium bicarbonate cotransporter; Sodium bicarbonate cotransporter kidney; sodium bicarbonate cotransporter member 4; Sodium bicarbonate cotransporter pancreas; Solute carrier family 4 member 4; Solute carrier family 4 sodium bicarbonate cotransporter member 4; Solute carrier family 4 sodium bicarbonate cotransporter member 5; Solute carrier family 4, sodium bicarbonate cotransporter, member 4, brain type;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y6R1 S4A4_HUMAN:

Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.

Description:
This gene encodes a sodium bicarbonate cotransporter (NBC) involved in the regulation of bicarbonate secretion and absorption and intracellular pH. Mutations in this gene are associated with proximal renal tubular acidosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Sequence:
MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKTGHKEKKEKERISENYSDKSDIENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIVDHQIETGLLKPELKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFTNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDNNFDYLEFRLWIGLWSAFLCLILVATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSNFKVGYNTLFSCTCVPPDPANISISNDTTLAPEYLPTMSSTDMYHNTTFDWAFLSKKECSKYGGNLVGNNCNFVPDITLMSFILFLGTYTSSMALKKFKTSPYFPTTARKLISDFAIILSILIFCVIDALVGVDTPKLIVPSEFKPTSPNRGWFVPPFGENPWWVCLAAAIPALLVTILIFMDQQITAVIVNRKEHKLKKGAGYHLDLFWVAILMVICSLMALPWYVAATVISIAHIDSLKMETETSAPGEQPKFLGVREQRVTGTLVFILTGLSVFMAPILKFIPMPVLYGVFLYMGVASLNGVQFMDRLKLLLMPLKHQPDFIYLRHVPLRRVHLFTFLQVLCLALLWILKSTVAAIIFPVMILALVAVRKGMDYLFSQHDLSFLDDVIPEKDKKKKEDEKKKKKKKGSLDSDNDDSDCPYSEKVPSIKIPMDIMEQQPFLSDSKPSDRERSPTFLERHTSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
82
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6R1 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
T28 Phosphorylation
Y30 Phosphorylation
T49 Phosphorylation Q9UEW8 (STK39)
S61 Phosphorylation
Y64 Phosphorylation
S65 Phosphorylation Q9UEW8 (STK39)
S68 Phosphorylation
K167 Acetylation
Y206 Phosphorylation
T207 Phosphorylation
S223 Phosphorylation
K229 Ubiquitination
S232 Phosphorylation
S233 Phosphorylation
S235 Phosphorylation
T239 Phosphorylation
S245 Phosphorylation
T249 Phosphorylation
T254 Phosphorylation
S255 Phosphorylation
S256 Phosphorylation
S257 Phosphorylation
S396 Phosphorylation
Y407 Phosphorylation
N641 N-Glycosylation
N661 N-Glycosylation
Y941 Phosphorylation
S1026 Phosphorylation P17612 (PRKACA)
S1029 Phosphorylation
S1034 Phosphorylation
Y1038 Phosphorylation
S1044 Phosphorylation
S1061 Phosphorylation
S1064 Phosphorylation
S1069 Phosphorylation
T1071 Phosphorylation
T1077 Phosphorylation
S1078 Phosphorylation

Research Backgrounds

Function:

Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.

May have a higher activity than isoform 1.

PTMs:

Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylated in presence of STK39 and dephosphorylated in presence of PP1 phosphatase; phosphorylation seems to inhibit SLC4A4 activity.

N-glycosylation is not necessary for the transporter basic functions.

Subcellular Location:

Basolateral cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal chord. Corneal endothelium cells express only isoform 1 (at protein level). Isoform 2 is specifically expressed in kidney at the level of proximal tubules.

Subunit Structure:

Homodimer. Interacts with CA2/carbonic anhydrase 2 and CA4/carbonic anhydrase 4 which may regulate transporter activity. Isoform 1 but not isoform 2 interacts with AHCYL1 (via PEST domain when phosphorylated); the interaction increases SLC4A4 isoform 1 activity. Interacts with AHCYL2 (By similarity).

Family&Domains:

Belongs to the anion exchanger (TC 2.A.31) family.

Research Fields

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

· Organismal Systems > Digestive system > Pancreatic secretion.

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