NDRG2 Antibody - #DF7272

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Product: NDRG2 Antibody
Catalog: DF7272
Description: Rabbit polyclonal antibody to NDRG2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 41kDa; 41kD(Calculated).
Uniprot: Q9UN36
RRID: AB_2839211

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NDRG2 Antibody detects endogenous levels of total NDRG2.
RRID:
AB_2839211
Cite Format: Affinity Biosciences Cat# DF7272, RRID:AB_2839211.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antidepressant related protein ADRG123; Cytoplasmic protein Ndr1; DKFZp781G1938; FLJ25522; KIAA1248; N myc downstream regulated gene 2; N myc downstream regulator 2; NDR1 related protein NDR2; NDRG 2; NDRG family member 2; NDRG1 related protein; NDRG2; NDRG2_HUMAN; Protein NDRG2; Protein Syld709613; SYLD; Syld709613 protein;

Immunogens

Immunogen:
Uniprot:

Q9UN36(NDRG2_HUMAN) >>Visit HPA database.

Gene(ID):
NDRG2(57447)
Expression:
Q9UN36 NDRG2_HUMAN:

Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level).

Description:
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Sequence:
MAELQEVQITEEKPLLPGQTPEAAKEAELAARILLDQGQTHSVETPYGSVTFTVYGTPKPKRPAILTYHDVGLNYKSCFQPLFQFEDMQEIIQNFVRVHVDAPGMEEGAPVFPLGYQYPSLDQLADMIPCVLQYLNFSTIIGVGVGAGAYILARYALNHPDTVEGLVLINIDPNAKGWMDWAAHKLTGLTSSIPEMILGHLFSQEELSGNSELIQKYRNIITHAPNLDNIELYWNSYNNRRDLNFERGGDITLRCPVMLVVGDQAPHEDAVVECNSKLDPTQTSFLKMADSGGQPQLTQPGKLTEAFKYFLQGMGYMASSCMTRLSRSRTASLTSAASVDGNRSRSRTLSQSSESGTLSSGPPGHTMEVSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UN36 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T20 Phosphorylation
Y47 Phosphorylation
T57 Phosphorylation
R247 Methylation
S276 Phosphorylation
Y309 Phosphorylation
S319 Phosphorylation
T323 Phosphorylation
S326 Phosphorylation
S328 Phosphorylation
T330 Phosphorylation O00141 (SGK1)
S332 Phosphorylation P23443 (RPS6KB1) , O00141 (SGK1) , P31749 (AKT1) , Q15418 (RPS6KA1)
T334 Phosphorylation
S335 Phosphorylation
S338 Phosphorylation
S344 Phosphorylation
S346 Phosphorylation O00141 (SGK1)
T348 Phosphorylation O00141 (SGK1) , P31749 (AKT1)
S350 Phosphorylation Q15418 (RPS6KA1) , P23443 (RPS6KB1)
S352 Phosphorylation
S353 Phosphorylation
S355 Phosphorylation
T357 Phosphorylation
S359 Phosphorylation
S360 Phosphorylation
T366 Phosphorylation
S370 Phosphorylation

Research Backgrounds

Function:

Contributes to the regulation of the Wnt signaling pathway. Down-regulates CTNNB1-mediated transcriptional activation of target genes, such as CCND1, and may thereby act as tumor suppressor. May be involved in dendritic cell and neuron differentiation.

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Cell projection>Growth cone.
Note: In neurons, seems to concentrate at axonal growth cone. Perinuclear in neurons (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level).

Subunit Structure:

Interacts with CTNNB1.

Family&Domains:

Belongs to the NDRG family.

References

1). Inhibition of PI3K/Akt/mTOR signaling by NDRG2 contributes to neuronal apoptosis and autophagy in ischemic stroke. Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, 2023 (PubMed: 36652790) [IF=2.5]

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