DDX5 Antibody - #DF7258

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Product: DDX5 Antibody
Catalog: DF7258
Description: Rabbit polyclonal antibody to DDX5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 69kDa; 69kD(Calculated).
Uniprot: P17844
RRID: AB_2839197

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
DDX5 Antibody detects endogenous levels of total DDX5.
RRID:
AB_2839197
Cite Format: Affinity Biosciences Cat# DF7258, RRID:AB_2839197.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent RNA helicase DDX5; DDX 5; Ddx5; DDX5_HUMAN; DEAD (Asp Glu Ala Asp) box helicase 5; DEAD (Asp Glu Ala Asp) box polypeptide 5; DEAD box 5; DEAD box protein 5; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD); G17P1; HELR; HLR1; HUMP68; P68; p68 RNA helicase; Probable ATP dependent RNA helicase DDX5; Probable ATP-dependent RNA helicase DDX5; RNA helicase p68;

Immunogens

Immunogen:
Uniprot:

P17844(DDX5_HUMAN) >>Visit HPA database.

Gene(ID):
DDX5(1655)
Description:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.
Sequence:
MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
82
Xenopus
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17844 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S5 Phosphorylation
S6 Phosphorylation
R10 Methylation
R12 Methylation
R14 Methylation
R20 Methylation
S24 Phosphorylation
R25 Methylation
S30 Phosphorylation
K32 Acetylation
K32 Ubiquitination
K33 Acetylation
K33 Ubiquitination
K40 Acetylation
K40 Ubiquitination
K43 Acetylation
K44 Acetylation
K45 Acetylation
K45 Sumoylation
K45 Ubiquitination
K53 Acetylation
K53 Sumoylation
K53 Ubiquitination
K56 Acetylation
K56 Methylation
K56 Sumoylation
K56 Ubiquitination
Y59 Phosphorylation
S79 Phosphorylation
K80 Acetylation
K80 Ubiquitination
T83 Phosphorylation
K91 Ubiquitination
Y97 Phosphorylation
K144 Ubiquitination
K197 Methylation
K197 Ubiquitination
S198 Phosphorylation P49137 (MAPKAPK2)
T199 Phosphorylation
C200 S-Nitrosylation
Y202 Phosphorylation
K207 Sumoylation
K207 Ubiquitination
T224 Phosphorylation
C234 S-Nitrosylation
K236 Acetylation
K236 Ubiquitination
T243 Phosphorylation
Y244 Phosphorylation
R263 Methylation
K264 Acetylation
K264 Ubiquitination
R273 Methylation
S279 Phosphorylation
K284 Ubiquitination
Y297 Phosphorylation
K328 Ubiquitination
S338 Phosphorylation
K340 Acetylation
K340 Methylation
K340 Ubiquitination
K343 Ubiquitination
T344 Phosphorylation
K351 Acetylation
K351 Ubiquitination
K375 Ubiquitination
K388 Acetylation
K388 Ubiquitination
K391 Sumoylation
K391 Ubiquitination
S402 Phosphorylation
R403 Methylation
K411 Sumoylation
K411 Ubiquitination
Y416 Phosphorylation
S421 Phosphorylation
Y425 Phosphorylation
K437 Sumoylation
K437 Ubiquitination
T438 Phosphorylation
T440 Phosphorylation
Y442 Phosphorylation
T446 Phosphorylation Q16539 (MAPK14)
K451 Ubiquitination
S454 Phosphorylation
S458 Phosphorylation
K470 Acetylation
K470 Sumoylation
K470 Ubiquitination
R478 Methylation
S480 Phosphorylation
R484 Methylation
R486 Methylation
K490 Methylation
S498 Phosphorylation
K501 Acetylation
R502 Methylation
T507 Phosphorylation
R509 Methylation
Y514 Phosphorylation
R516 Methylation
Y518 Phosphorylation
S519 Phosphorylation
S520 Phosphorylation
K523 Sumoylation
K523 Ubiquitination
Y535 Phosphorylation
S557 Phosphorylation
T564 Phosphorylation Q16539 (MAPK14)
Y593 Phosphorylation P00519 (ABL1)
Y595 Phosphorylation

Research Backgrounds

Function:

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.

PTMs:

Arg-502 is dimethylated, probably to asymmetric dimethylarginine.

Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination.

Polyubiquitinated, leading to proteasomal degradation.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in the spliceosome C complex. Interacts with RBM4; the interaction occurs in an RNA-independent manner. Interacts with AGO1 and AGO2. Interacts with ESR1, AR, EP300, CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interacts with DDX17. Interacts with BRDT. The large PER complex involved in the repression of transcriptional termination is composed of at least PER2, CDK9, DDX5, DHX9, NCBP1 and POLR2A (active). Interacts with DHX36; this interaction occurs in a RNA-dependent manner. Interacts with NUPR1 (By similarity). Interacts with ERCC6.

Family&Domains:

Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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