RRM2 Antibody - #DF7248

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Product: RRM2 Antibody
Catalog: DF7248
Description: Rabbit polyclonal antibody to RRM2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Chicken
Mol.Wt.: 45kDa; 45kD(Calculated).
Uniprot: P31350
RRID: AB_2839187

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
RRM2 Antibody detects endogenous levels of total RRM2.
RRID:
AB_2839187
Cite Format: Affinity Biosciences Cat# DF7248, RRID:AB_2839187.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cb111; chunp6884; etID309896.20; R2; reductase M2 polypeptide variant 1; reductase M2 polypeptide variant 2; reductase M2 polypeptide variant 3a; reductase M2 polypeptide variant 3b; reductase M2 polypeptide variant 3c; reductase M2 polypeptide variant 3d; Ribonucleoside-diphosphate reductase subunit M2; Ribonucleotide reductase M2; Ribonucleotide reductase M2 polypeptide; Ribonucleotide reductase M2 subunit; ribonucleotide reductase protein r2 class I; Ribonucleotide reductase regulatory subunit M2; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit; Ribonucleotide reductase, R2 subunit; RIR2_HUMAN; RR2; RR2M; RRM2;

Immunogens

Immunogen:
Uniprot:

P31350(RIR2_HUMAN) >>Visit HPA database.

Gene(ID):
RRM2(6241)
Description:
Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit.
Sequence:
MLSLRVPLAPITDPQQLQLSPLKGLSLVDKENTPPALSGTRVLASKTARRIFQEPTEPKTKAAAPGVEDEPLLRENPRRFVIFPIEYHDIWQMYKKAEASFWTAEEVDLSKDIQHWESLKPEERYFISHVLAFFAASDGIVNENLVERFSQEVQITEARCFYGFQIAMENIHSEMYSLLIDTYIKDPKEREFLFNAIETMPCVKKKADWALRWIGDKEATYGERVVAFAAVEGIFFSGSFASIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFKHLVHKPSEERVREIIINAVRIEQEFLTEALPVKLIGMNCTLMKQYIEFVADRLMLELGFSKVFRVENPFDFMENISLEGKTNFFEKRVGEYQRMGVMSSPTENSFTLDADF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Chicken
83
Zebrafish
67
Pig
0
Sheep
0
Dog
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31350 As Substrate

Site PTM Type Enzyme
T12 Phosphorylation
S20 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
K23 Ubiquitination
S26 Phosphorylation
K30 Ubiquitination
T33 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
S38 Phosphorylation
T40 Phosphorylation
S45 Phosphorylation
K46 Ubiquitination
T47 Phosphorylation
T56 Phosphorylation
K59 Ubiquitination
K61 Ubiquitination
K120 Ubiquitination
K204 Ubiquitination
K205 Ubiquitination
K206 Ubiquitination
R212 Methylation
K217 Ubiquitination
Y221 Phosphorylation
S263 Phosphorylation
K278 Ubiquitination
K283 Ubiquitination
K311 Ubiquitination
T318 Phosphorylation
K358 Ubiquitination
K364 Acetylation
K364 Ubiquitination
Y369 Phosphorylation
S377 Phosphorylation

Research Backgrounds

Function:

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.

PTMs:

Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer of a large and a small subunit.

Family&Domains:

Belongs to the ribonucleoside diphosphate reductase small chain family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Gambogic acid sensitizes gemcitabine efficacy in pancreatic cancer by reducing the expression of ribonucleotide reductase subunit-M2 (RRM2). Journal of Experimental & Clinical Cancer Research, 2017 (PubMed: 28797284) [IF=11.3]

Application: WB    Species: human    Sample:

2). Sharp Downregulation of Hub Genes Associated With the Pathogenesis of Breast Cancer From Ductal Carcinoma In Situ to Invasive Ductal Carcinoma. Frontiers in Oncology, 2021 (PubMed: 34094915) [IF=4.7]

Application: WB    Species: Human    Sample: breast cancer tissue

Figure 6 Protein expression levels of hub genes. (A) Protein expression levels of CDK1, MELK, CEP55, TOP2A, NUSAP1, PBK, RRM2, and MAD2L1 were determined by western blotting. (B) Western blot analysis of CDK1, MELK, CEP55, TOP2A, NUSAP1, PBK, RRM2, and MAD2L1 in tissue from different stages of breast disease, and quantification of the intensity relative to GAPDH. One-way ANOVA was performed to acquire statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). NME, normal mammary epithelium; SH, simple ductal hyperplasia; ADH, atypical ductal hyperplasia; DCIS, ductal carcinoma in situ; IDC, invasive ductal carcinoma.

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