NTF3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HT-29 , using NTF3antibody.

Product:	NTF3 Antibody
Catalog:	DF7235
Description:	Rabbit polyclonal antibody to NTF3
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	31kDa; 29kD(Calculated).
Uniprot:	P20783
RRID:	AB_2839175

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

NTF3 Antibody detects endogenous levels of total NTF3.

RRID:

AB_2839175
Cite Format: Affinity Biosciences Cat# DF7235, RRID:AB_2839175.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; Neurotrophin 3 precursor; Neurotrophin-3; Neurotrophin3; NGF 2; NGF-2; NGF2; NT 3; NT-3; NT3; NTF 3; NTF3; NTF3_HUMAN;

Immunogens

Immunogen:

Uniprot:

P20783(NTF3_HUMAN) >>Visit HPA database.

Gene(ID):

NTF3(4908)

Expression:

P20783 NTF3_HUMAN:

Brain and peripheral tissues.

Description:

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.

Sequence:

P20783 NTF3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSILFYVIFLAYLRGIQGNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPERGGPAKSAFQPVIAMDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGSPVVANRTSRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20783 As Substrate

P20783

Site	PTM Type	Source
Y59	Phosphorylation	Uniprot
Y99	Phosphorylation	Uniprot
S101	Phosphorylation	Uniprot
Y123	Phosphorylation	Uniprot
T222	Phosphorylation	Uniprot

Research Backgrounds

P20783_NTF3_HUMAN

Function:

Seems to promote the survival of visceral and proprioceptive sensory neurons.

Subcellular Location:

Secreted.

Tissue Specificity:

Brain and peripheral tissues.

Family&Domains:

Belongs to the NGF-beta family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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NTF3 Antibody - #DF7235