MSH6 Antibody - #DF7233
Product: | MSH6 Antibody |
Catalog: | DF7233 |
Description: | Rabbit polyclonal antibody to MSH6 |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Bovine, Sheep, Rabbit, Dog |
Mol.Wt.: | 153kDa; 153kD(Calculated). |
Uniprot: | P52701 |
RRID: | AB_2839173 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF7233, RRID:AB_2839173.
Fold/Unfold
DNA mismatch repair protein Msh6; G/T mismatch binding protein; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC 5; HNPCC5; HSAP; MSH 6; MSH6; MSH6_HUMAN; mutS (E. coli) homolog 6; MutS alpha 160 kDa subunit; MutS homolog 6 (E. coli); mutS homolog 6; MutS-alpha 160 kDa subunit; p160; Sperm associated protein;
Immunogens
- P52701 MSH6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPEDFLNSCTPGMRKWWQIKSQNFDLVICYKVGKFYELYHMDALIGVSELGLVFMKGNWAHSGFPEIAFGRYSDSLVQKGYKVARVEQTETPEMMEARCRKMAHISKYDRVVRREICRIITKGTQTYSVLEGDPSENYSKYLLSLKEKEEDSSGHTRAYGVCFVDTSLGKFFIGQFSDDRHCSRFRTLVAHYPPVQVLFEKGNLSKETKTILKSSLSCSLQEGLIPGSQFWDASKTLRTLLEEEYFREKLSDGIGVMLPQVLKGMTSESDSIGLTPGEKSELALSALGGCVFYLKKCLIDQELLSMANFEEYIPLDSDTVSTTRSGAIFTKAYQRMVLDAVTLNNLEIFLNGTNGSTEGTLLERVDTCHTPFGKRLLKQWLCAPLCNHYAINDRLDAIEDLMVVPDKISEVVELLKKLPDLERLLSKIHNVGSPLKSQNHPDSRAIMYEETTYSKKKIIDFLSALEGFKVMCKIIGIMEEVADGFKSKILKQVISLQTKNPEGRFPDLTVELNRWDTAFDHEKARKTGLITPKAGFDSDYDQALADIRENEQSLLEYLEKQRNRIGCRTIVYWGIGRNRYQLEIPENFTTRNLPEEYELKSTKKGCKRYWTKTIEKKLANLINAEERRDVSLKDCMRRLFYNFDKNYKDWQSAVECIAVLDVLLCLANYSRGGDGPMCRPVILLPEDTPPFLELKGSRHPCITKTFFGDDFIPNDILIGCEEEEQENGKAYCVLVTGPNMGGKSTLMRQAGLLAVMAQMGCYVPAEVCRLTPIDRVFTRLGASDRIMSGESTFFVELSETASILMHATAHSLVLVDELGRGTATFDGTAIANAVVKELAETIKCRTLFSTHYHSLVEDYSQNVAVRLGHMACMVENECEDPSQETITFLYKFIKGACPKSYGFNAARLANLPEEVIQKGHRKAREFEKMNQSLRLFREVCLASERSTVDAEAVHKLLTLIKEL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P52701 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
Y8 | Phosphorylation | Uniprot | |
S9 | Phosphorylation | Uniprot | |
S14 | Phosphorylation | Uniprot | |
K22 | Ubiquitination | Uniprot | |
R33 | Methylation | Uniprot | |
S41 | Phosphorylation | Uniprot | |
S43 | Phosphorylation | Uniprot | |
S51 | Phosphorylation | Uniprot | |
S63 | Phosphorylation | Uniprot | |
S65 | Phosphorylation | Uniprot | |
K70 | Acetylation | Uniprot | |
S79 | Phosphorylation | Uniprot | |
T86 | Phosphorylation | Uniprot | |
S87 | Phosphorylation | Uniprot | |
S91 | Phosphorylation | Uniprot | |
S137 | Phosphorylation | Uniprot | |
T139 | Phosphorylation | Uniprot | |
R140 | Methylation | Uniprot | |
S144 | Phosphorylation | Uniprot | |
Y151 | Phosphorylation | Uniprot | |
K155 | Acetylation | Uniprot | |
K161 | Ubiquitination | Uniprot | |
K169 | Sumoylation | Uniprot | |
K169 | Ubiquitination | Uniprot | |
S200 | Phosphorylation | Uniprot | |
T212 | Phosphorylation | Uniprot | |
T213 | Phosphorylation | Uniprot | |
Y214 | Phosphorylation | Uniprot | |
T216 | Phosphorylation | Uniprot | |
S219 | Phosphorylation | Uniprot | |
S227 | Phosphorylation | Uniprot | |
T235 | Phosphorylation | Uniprot | |
S252 | Phosphorylation | Uniprot | |
S254 | Phosphorylation | Uniprot | |
S256 | Phosphorylation | Uniprot | |
S261 | Phosphorylation | Uniprot | |
T269 | Phosphorylation | Uniprot | |
S274 | Phosphorylation | Uniprot | |
S275 | Phosphorylation | Uniprot | |
S279 | Phosphorylation | Uniprot | |
S280 | Phosphorylation | Uniprot | |
S285 | Phosphorylation | Uniprot | |
S292 | Phosphorylation | Uniprot | |
T305 | Phosphorylation | Uniprot | |
S309 | Phosphorylation | Uniprot | |
K324 | Ubiquitination | Uniprot | |
K334 | Sumoylation | Uniprot | |
K334 | Ubiquitination | Uniprot | |
S348 | Phosphorylation | Uniprot | |
K374 | Ubiquitination | Uniprot | |
K476 | Ubiquitination | Uniprot | |
K479 | Ubiquitination | Uniprot | |
T488 | Phosphorylation | Uniprot | |
K498 | Ubiquitination | Uniprot | |
K504 | Acetylation | Uniprot | |
K504 | Ubiquitination | Uniprot | |
K519 | Ubiquitination | Uniprot | |
K537 | Ubiquitination | Uniprot | |
K543 | Ubiquitination | Uniprot | |
R577 | Methylation | Uniprot | |
K598 | Ubiquitination | Uniprot | |
K603 | Ubiquitination | Uniprot | |
K610 | Ubiquitination | Uniprot | |
K632 | Ubiquitination | Uniprot | |
Y642 | Phosphorylation | Uniprot | |
K646 | Ubiquitination | Uniprot | |
K660 | Ubiquitination | Uniprot | |
S668 | Phosphorylation | Uniprot | |
K693 | Ubiquitination | Uniprot | |
S702 | Phosphorylation | Uniprot | |
Y709 | Phosphorylation | Uniprot | |
K728 | Ubiquitination | Uniprot | |
T767 | Phosphorylation | Uniprot | |
K771 | Ubiquitination | Uniprot | |
K813 | Ubiquitination | Uniprot | |
K814 | Ubiquitination | Uniprot | |
K824 | Ubiquitination | Uniprot | |
S830 | Phosphorylation | Uniprot | |
K833 | Methylation | Uniprot | |
K833 | Ubiquitination | Uniprot | |
K852 | Ubiquitination | Uniprot | |
K854 | Ubiquitination | Uniprot | |
K866 | Ubiquitination | Uniprot | |
K870 | Ubiquitination | Uniprot | |
K883 | Ubiquitination | Uniprot | |
K888 | Ubiquitination | Uniprot | |
T895 | Phosphorylation | Uniprot | |
K896 | Ubiquitination | Uniprot | |
T914 | Phosphorylation | Uniprot | |
K920 | Ubiquitination | Uniprot | |
K923 | Ubiquitination | Uniprot | |
T924 | Phosphorylation | Uniprot | |
T928 | Phosphorylation | Uniprot | |
K930 | Ubiquitination | Uniprot | |
S935 | Phosphorylation | Uniprot | |
K957 | Ubiquitination | Uniprot | |
K997 | Sumoylation | Uniprot | |
K997 | Ubiquitination | Uniprot | |
K1009 | Ubiquitination | Uniprot | |
T1010 | Phosphorylation | Uniprot | |
K1014 | Ubiquitination | Uniprot | |
K1030 | Ubiquitination | Uniprot | |
T1085 | Phosphorylation | Uniprot | |
K1092 | Methylation | Uniprot | |
K1092 | Ubiquitination | Uniprot | |
K1101 | Ubiquitination | Uniprot | |
K1126 | Methylation | Uniprot | |
K1140 | Ubiquitination | Uniprot | |
R1172 | Methylation | Uniprot | |
K1240 | Ubiquitination | Uniprot | |
K1291 | Ubiquitination | Uniprot | |
K1296 | Ubiquitination | Uniprot | |
K1315 | Ubiquitination | Uniprot | |
K1325 | Ubiquitination | Uniprot | |
K1352 | Ubiquitination | Uniprot | |
K1358 | Ubiquitination | Uniprot |
Research Backgrounds
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
The N-terminus is blocked.
Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
Nucleus. Chromosome.
Note: Associates with H3K36me3 via its PWWP domain.
Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with MCM9. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains.
(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.
The PWWP domain specifically recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3).
Belongs to the DNA mismatch repair MutS family.
Research Fields
· Genetic Information Processing > Replication and repair > Mismatch repair.
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)
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