DNMT3A Antibody - #DF7226

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Product: DNMT3A Antibody
Catalog: DF7226
Description: Rabbit polyclonal antibody to DNMT3A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 120-140kD; 102kD(Calculated).
Uniprot: Q9Y6K1
RRID: AB_2839167

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(91%), Horse(82%), Rabbit(91%), Dog(82%)
Clonality:
Polyclonal
Specificity:
DNMT3A Antibody detects endogenous levels of total DNMT3A.
RRID:
AB_2839167
Cite Format: Affinity Biosciences Cat# DF7226, RRID:AB_2839167.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA (cytosine 5) methyltransferase 3 alpha; DNA (cytosine 5) methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3 alpha; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; DNM3A_HUMAN; DNMT 3a; DNMT; Dnmt3a; DNMT3A2; M.HsaIIIA; MCMT; OTTHUMP00000201149; TBRS;

Immunogens

Immunogen:
Uniprot:

Q9Y6K1(DNM3A_HUMAN) >>Visit HPA database.

Gene(ID):
DNMT3A(1788)
Expression:
Q9Y6K1 DNM3A_HUMAN:

Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Description:
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms.
Sequence:
MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTARKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEGSPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAGMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAVEVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Bovine
91
Pig
82
Horse
82
Dog
82
Chicken
64
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6K1 As Substrate

Site PTM Type Enzyme
R23 Methylation
T65 Phosphorylation
S75 Phosphorylation
S105 Phosphorylation
T138 Phosphorylation
T156 Phosphorylation
R171 Methylation
S178 Phosphorylation
S179 Phosphorylation
S199 Phosphorylation
K200 Ubiquitination
S236 Phosphorylation
S243 Phosphorylation
S255 Phosphorylation
T261 Phosphorylation
S267 Phosphorylation
K276 Ubiquitination
K299 Ubiquitination
K367 Ubiquitination
S377 Phosphorylation
K382 Ubiquitination
S390 Phosphorylation
S393 Phosphorylation
K420 Ubiquitination
K468 Sumoylation
S638 Phosphorylation
T645 Phosphorylation
K721 Ubiquitination
K783 Ubiquitination
K812 Ubiquitination
S839 Phosphorylation
K844 Ubiquitination
S894 Phosphorylation
K906 Ubiquitination

Research Backgrounds

Function:

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

PTMs:

Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Accumulates in the major satellite repeats at pericentric heterochromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Subunit Structure:

Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with UBC9, PIAS1 and PIAS2 (By similarity). Binds the ZBTB18 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain. Interacts with UHRF1 (By similarity). Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Interacts with MPHOSPH8. Interacts with histone H3 that is not methylated at 'Lys-4' (H3K4).

Family&Domains:

The PWWP domain is essential for targeting to pericentric heterochromatin. It specifically recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3) (By similarity).

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Total Barley Maiya Alkaloids Prevent Increased Prolactin Levels Caused by Antipsychotic Drugs and Reduce Dopamine Receptor D2 via Epigenetic Mechanisms. Frontiers in Pharmacology, 2022 (PubMed: 35865960) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 4 Effects of TBMA, hordenine, and N-methyltyramine on DRD2 and DNMT expression. (A–E) DRD2, DNMT1, DNMT3α, and DNMT3β protein expression. GAPDH was used as a loading control. (F) DRD2, DNMT1, DNMT3α, and DNMT3β mRNA expression. Data are presented as the mean ± SD. Protein expression in each group was determined using western blotting. mRNA expression in each group was determined using RT-PCR (n = 3 rats per group; # p < 0.05 and ## p < 0.01 versus the control group; * p < 0.05 versus the Model group. Bro = bromocriptine; Hor = hordenine; N-Methy = N-methyltyramine; Ris = risperidone; TBMA = total barley maiya alkaloids.
2). Inhibitory effect of bound polyphenol from foxtail millet bran on miR-149 methylation increases the chemosensitivity of human colorectal cancer HCT-8/Fu cells. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2021 (PubMed: 33011952) [IF=4.3]

Application: WB    Species: human    Sample: HCT-8 and HCT-8/Fu cells

Fig. 4 |Efects of BPIS on the expression of DNA methylation-related enzymes. a, b The expressions of DNMT3a, DNMT3b and MECP2 were measured in RNA and protein levels in HCT-8 and HCT-8/Fu cells by RT-PCR and western blotting assays.
3). Decreased ovarian function and autophagy gene methylation in aging rats. Journal of Ovarian Research, 2020 (PubMed: 32014030) [IF=4.0]

Application: WB    Species: rat    Sample: ovaries

Fig. 6| Protein expression of Dnmt1, Dnmt2, Dnmt3A and Dnmt3B in the ovaries of rats.(a) Western blot results.

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