SFTPA1 Antibody - #DF7204

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Product: SFTPA1 Antibody
Catalog: DF7204
Description: Rabbit polyclonal antibody to SFTPA1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 28kDa; 26kD(Calculated).
Uniprot: Q8IWL2
RRID: AB_2839155

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(82%), Horse(90%), Sheep(82%), Rabbit(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
SFTPA1 Antibody detects endogenous levels of total SFTPA1.
RRID:
AB_2839155
Cite Format: Affinity Biosciences Cat# DF7204, RRID:AB_2839155.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

35 kDa pulmonary surfactant associated protein; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; COLEC4; Collectin 4; Collectin-4; FLJ50593; FLJ51913; FLJ61144; FLJ77898,; FLJ79095; FLJ99559; MGC133365; MGC198590; OTTHUMP00000019928; OTTHUMP00000019929; OTTHUMP00000019930; OTTHUMP00000019931; PSAP; PSP A; PSP-A; PSPA; pulmonary surfactant -associated protein, 35-KD; pulmonary surfactant apoprotein PSP-A; pulmonary surfactant associated protein A1; Pulmonary surfactant-associated protein A1; SFTA1_HUMAN; SFTP1; SFTPA1; SFTPA1B; SP A; SP A1; SP-A; SP-A1; SPA; SPA1; surfactant protein A1; surfactant protein A1 variant AB'D' 6A2; surfactant protein A1 variant AB'D' 6A3; surfactant protein A1 variant AB'D' 6A4; surfactant protein A1 variant ACD' 6A2; surfactant protein A1 variant ACD' 6A3; surfactant protein A1 variant ACD' 6A4; surfactant protein A1 variant AD' 6A; surfactant protein A1 variant AD' 6A2; surfactant protein A1 variant AD' 6A3; surfactant protein A1 variant AD' 6A4; surfactant protein A1B; surfactant, pulmonary associated protein A1A; surfactant, pulmonary associated protein A1B; surfactant-associated protein, pulmonary 1;

Immunogens

Immunogen:
Uniprot:

Q8IWL2(SFTA1_HUMAN) >>Visit HPA database.

Gene(ID):
SFTPA1(653509)
Description:
This gene encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants.
Sequence:
MWLCPLALNLILMAASGAVCEVKDVCVGSPGIPGTPGSHGLPGRDGRDGLKGDPGPPGPMGPPGEMPCPPGNDGLPGAPGIPGECGEKGEPGERGPPGLPAHLDEELQATLHDFRHQILQTRGALSLQGSIMTVGEKVFSSNGQSITFDAIQEACARAGGRIAVPRNPEENEAIASFVKKYNTYAYVGLTEGPSPGDFRYSDGTPVNYTNWYRGEPAGRGKEQCVEMYTDGQWNDRNCLYSRLTICEF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Dog
91
Horse
90
Bovine
82
Sheep
82
Pig
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IWL2 As Substrate

Site PTM Type Enzyme
Y184 Phosphorylation
S241 Phosphorylation

Research Backgrounds

Function:

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages (By similarity).

(Microbial infection) Recognition of M.tuberculosis by dendritic cells may occur partially via this molecule. Can recognize, bind, and opsonize pathogens to enhance their elimination by alveolar macrophages.

PTMs:

N-acetylated.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix. Secreted>Extracellular space>Surface film.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Oligomeric complex of 6 set of homotrimers.

(Microbial infection) Binds M.bovis cell surface protein Apa via its glycosylated sites; probably also recognizes other bacterial moieties.

(Microbial infection) Binds to the S.aureus extracellular adherence protein, Eap, thereby enhancing phagocytosis and killing of S.aureus by alveolar macrophages.

Family&Domains:

Belongs to the SFTPA family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

References

1). miR-296-5p Inhibits the Secretion of Pulmonary Surfactants in Pulmonary Epithelial Cells via the Downregulation of Wnt7b/β-Catenin Signaling. Biomed Research International, 2021 (PubMed: 33490270)

Application: WB    Species: Human    Sample: A549 cell

Figure 4 miR-296-5p inhibited the expression of SP-A (SFTPA1) and SP-B through the Wnt signaling pathway. After transfection of mir-296-5p overexpression and empty vectors in the A549 cell line, the expression of WNT7B, β-catenin, SP-A, and SP-B was assessed using western blotting.

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