SLC22A8 Antibody - #DF7196

(6)   (9)  
Product: SLC22A8 Antibody
Catalog: DF7196
Description: Rabbit polyclonal antibody to SLC22A8
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 60kDa; 60kD(Calculated).
Uniprot: Q8TCC7
RRID: AB_2839148

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:25-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(100%)
Clonality:
Polyclonal
Specificity:
SLC22A8 Antibody detects endogenous levels of total SLC22A8.
RRID:
AB_2839148
Cite Format: Affinity Biosciences Cat# DF7196, RRID:AB_2839148.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hOAT3; OAT3; Organic anion transporter 3; S22A8_HUMAN; SLC22A8; Solute carrier family 22 (organic anion transporter), member 8; Solute carrier family 22 member 8;

Immunogens

Immunogen:
Uniprot:

Q8TCC7(S22A8_HUMAN) >>Visit HPA database.

Gene(ID):
SLC22A8(9376)
Expression:
Q8TCC7 S22A8_HUMAN:

Expressed in kidney.

Description:
This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
Sequence:
MTFSEILDRVGSMGHFQFLHVAILGLPILNMANHNLLQIFTAATPVHHCRPPHNASTGPWVLPMGPNGKPERCLRFVHPPNASLPNDTQRAMEPCLDGWVYNSTKDSIVTEWDLVCNSNKLKEMAQSIFMAGILIGGLVLGDLSDRFGRRPILTCSYLLLAASGSGAAFSPTFPIYMVFRFLCGFGISGITLSTVILNVEWVPTRMRAIMSTALGYCYTFGQFILPGLAYAIPQWRWLQLTVSIPFFVFFLSSWWTPESIRWLVLSGKSSKALKILRRVAVFNGKKEEGERLSLEELKLNLQKEISLAKAKYTASDLFRIPMLRRMTFCLSLAWFATGFAYYSLAMGVEEFGVNLYILQIIFGGVDVPAKFITILSLSYLGRHTTQAAALLLAGGAILALTFVPLDLQTVRTVLAVFGKGCLSSSFSCLFLYTSELYPTVIRQTGMGVSNLWTRVGSMVSPLVKITGEVQPFIPNIIYGITALLGGSAALFLPETLNQPLPETIEDLENWSLRAKKPKQEPEVEKASQRIPLQPHGPGLGSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
75
Dog
75
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TCC7 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
T439 Phosphorylation

Research Backgrounds

Function:

Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Involved in the transport basolateral of steviol, fexofenadine. Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA).

Subcellular Location:

Basolateral cell membrane>Multi-pass membrane protein.
Note: Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in kidney.

Family&Domains:

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

References

1). Caffeic acid phenethyl ester alleviated hypouricemia in hyperuricemic mice through inhibiting XOD and up-regulating OAT3. PHYTOMEDICINE, 2022 (PubMed: 35714456) [IF=7.9]
2). Hypouricemic effect of gallic acid, a bioactive compound from Sonneratia apetala leaves and branches, on hyperuricemic mice. Food & Function, 2022 (PubMed: 36125096) [IF=6.1]
3). Berberrubine attenuates potassium oxonate- and hypoxanthine-induced hyperuricemia by regulating urate transporters and JAK2/STAT3 signaling pathway. European Journal of Pharmacology, 2021 (PubMed: 34699754) [IF=5.0]
4). Xanthones from securidaca inappendiculata antagonized the anti-rheumatic effects of methotrexate in vivo by promoting its secretion into urine. Expert Opinion on Drug Metabolism & Toxicology, 2021 (PubMed: 33107357) [IF=4.3]

Application: WB    Species: rat    Sample: kidney

Figure 4. | XRF promoted MTX secretion into urine in CIA rats.; B, effects of treatments on OAT3 expression in kidney, investigated by the immunoblotting method, **p < 0.01 compared with CIA models, ##p < 0.01 compared with MTX treated rats
5). Hypouricaemic and nephroprotective effects of Poria cocos in hyperuricemic mice by up-regulating ATP-binding cassette super-family G member 2. PHARMACEUTICAL BIOLOGY, 2021 (PubMed: 33651969) [IF=3.8]

Application: WB    Species: Mice    Sample: kidney tissues

Figure 4. Effects of PCE and PCW on renal ABCG2, OAT3, OAT1 and OCT2 protein expression detected by Western blot: immunoreactive bands (a) and densitometries (b,c,d and –e, expressed as mean ± SD; n n ¼ 3).  p < 0.05,  p < 0.01 versus the normal control; #p < 0.05, ##p < 0.01 versus the hyperuricemic control;  p < 0.01 versus the allopurinol control.
6). CP-25 ameliorates methotrexate induced nephrotoxicity via improving renal apoptosis and methotrexate excretion. Journal of Pharmacological Sciences, 2021 (PubMed: 33858651) [IF=3.5]

Application: WB    Species: Rat    Sample: renal tissue

Fig. 4. Effects of CP-25 on renal OAT3 expression and function in vivo. A: the renal excretion of MTX were measured in rats that received a single intravenous injection of MTX (5 mg/kg). B: the tissue extracts were prepared, and the lysate was resolved by SDS-PAGE and probed with antibodies against OAT3. C: OAT3 function was evaluated by detecting DHEA excretion in the rats received a single intravenous injection of DHEA (20 mg/kg). The data are presented as the means ± S.D. (n ¼ 6). ##P < 0.01 compared with normal group; *P < 0.05, **P < 0.01 compared with the model group (MTX).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.