SUMO4 Antibody | Affinity Biosciences

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Western blot analysis of extracts from VERO cells, using SUMO4 Antibody.

DF7186 at 1/100 staining Rat brain tissue by IHC-P.

Product:	SUMO4 Antibody
Catalog:	DF7186
Description:	Rabbit polyclonal antibody to SUMO4
Application:	WB IHC
Reactivity:	Human, Mouse, Rat, Monkey
Mol.Wt.:	12kDa; 11kD(Calculated).
Uniprot:	Q6EEV6
RRID:	AB_2839138

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-1:2000, IHC 1:100-1:300
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Clonality:

Polyclonal

Specificity:

SUMO4 Antibody detects endogenous levels of total SUMO4.

RRID:

AB_2839138
Cite Format: Affinity Biosciences Cat# DF7186, RRID:AB_2839138.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

dJ281H8.4; IDDM5; Small ubiquitin like modifier 4 protein; Small ubiquitin-like protein 4; Small ubiquitin-related modifier 4; SMT3 suppressor of mif two 3 homolog 2; SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae); SMT3H4; SUMO 4; SUMO-4; SUMO4; SUMO4_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q6EEV6(SUMO4_HUMAN) >>Visit HPA database.

Gene(ID):

SUMO4(387082)

Expression:

Q6EEV6 SUMO4_HUMAN:

Expressed mainly in adult and embryonic kidney. Expressed at various levels in immune tissues, with the highest expression in the lymph node and spleen.

Description:

This gene is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism.

Sequence:

Q6EEV6 SUMO4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MANEKPTEEVKTENNNHINLKVAGQDGSVVQFKIKRQTPLSKLMKAYCEPRGLSMKQIRFRFGGQPISGTDKPAQLEMEDEDTIDVFQQPTGGVY

PTMs - Q6EEV6 As Substrate

Q6EEV6

Site	PTM Type	Source
K11	Sumoylation	Uniprot
K21	Ubiquitination	Uniprot
S28	Phosphorylation	Uniprot
K33	Acetylation	Uniprot
K33	Methylation	Uniprot
K33	Sumoylation	Uniprot
K33	Ubiquitination	Uniprot
K35	Ubiquitination	Uniprot

Research Backgrounds

Q6EEV6_SUMO4_HUMAN

Function:

Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I.

PTMs:

In contrast to SUMO1, SUMO2 and SUMO3, seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates.

Tissue Specificity:

Expressed mainly in adult and embryonic kidney. Expressed at various levels in immune tissues, with the highest expression in the lymph node and spleen.

Subunit Structure:

Interacts with SAE2. Covalently attached to a number of proteins (Probable).

Family&Domains:

Belongs to the ubiquitin family. SUMO subfamily.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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SUMO4 Antibody - #DF7186