TPM2 Antibody - #DF7183

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Product: TPM2 Antibody
Catalog: DF7183
Description: Rabbit polyclonal antibody to TPM2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 33kD; 33kD(Calculated).
Uniprot: P07951
RRID: AB_2839135

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(82%), Bovine(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TPM2 Antibody detects endogenous levels of total TPM2.
RRID:
AB_2839135
Cite Format: Affinity Biosciences Cat# DF7183, RRID:AB_2839135.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Alpha tropomyosin; AMCD1; Arthrogryposis multiplex congenital distal type 1; Beta tropomyosin; Beta-tropomyosin; Cytoskeletal tropomyosin TM30; DA1; DA2B; epididymis secretory protein Li 273; FLJ41118; Heat stable cytoskeletal protein 30 kDa; HEL-S-273; hscp30; HTM alpha; hTM5; MGC14582; MGC3261; MGC72094; NEM1; NEM4; Nemaline myopathy type 4; OK/SW cl.5; Sarcomeric tropomyosin kappa; TM 5; TM3; TM30; TM30nm; TMSA; TMSB; TPM 1; TPM 3; TPM1 alpha; TPM1 kappa; TPM2; TPM2_HUMAN; TRK; Tropomyosin 1 alpha; Tropomyosin 1 alpha chain; Tropomyosin 1 alpha chain isoform 6; Tropomyosin 2 (beta); Tropomyosin 2; Tropomyosin 3; Tropomyosin alpha 3 chain; Tropomyosin alpha striated muscle isoform; Tropomyosin beta chain; Tropomyosin gamma; Tropomyosin skeletal muscle beta; Tropomyosin-2;

Immunogens

Immunogen:
Uniprot:

P07951(TPM2_HUMAN) >>Visit HPA database.

Gene(ID):
TPM2(7169)
Expression:
P07951 TPM2_HUMAN:

Present in primary breast cancer tissue, absent from normal breast tissue.

Sequence:
MDAIKKKMQMLKLDKENAIDRAEQAEADKKQAEDRCKQLEEEQQALQKKLKGTEDEVEKYSESVKEAQEKLEQAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMELQEMQLKEAKHIAEDSDRKYEEVARKLVILEGELERSEERAEVAESKCGDLEEELKIVTNNLKSLEAQADKYSTKEDKYEEEIKLLEEKLKEAETRAEFAERSVAKLEKTIDDLEDEVYAQKMKYKAISEELDNALNDITSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
82
Pig
0
Horse
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07951 As Substrate

Site PTM Type Enzyme
K12 Acetylation
K12 Ubiquitination
K29 Acetylation
K30 Ubiquitination
K37 Ubiquitination
K48 Acetylation
K48 Ubiquitination
K51 Ubiquitination
T53 Phosphorylation
K59 Ubiquitination
Y60 Phosphorylation
S61 Phosphorylation
S63 Phosphorylation
K65 Ubiquitination
K76 Acetylation
K77 Ubiquitination
T79 Phosphorylation
S87 Phosphorylation
T108 Phosphorylation
K112 Acetylation
K112 Ubiquitination
K118 Acetylation
K118 Ubiquitination
S123 Phosphorylation
K128 Sumoylation
K128 Ubiquitination
K136 Ubiquitination
K140 Acetylation
K149 Ubiquitination
S158 Phosphorylation
Y162 Phosphorylation
K168 Ubiquitination
S188 Phosphorylation
K198 Ubiquitination
K205 Ubiquitination
S206 Phosphorylation
K213 Ubiquitination
K220 Acetylation
K220 Methylation
K220 Ubiquitination
Y221 Phosphorylation
K226 Ubiquitination
K231 Ubiquitination
K233 Ubiquitination
T237 Phosphorylation
S245 Phosphorylation
K248 Acetylation
K248 Ubiquitination
K251 Ubiquitination
T252 Phosphorylation
Y261 Phosphorylation
Y267 Phosphorylation
K268 Ubiquitination
T282 Phosphorylation
S283 Phosphorylation

Research Backgrounds

Function:

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.

PTMs:

Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: Associates with F-actin stress fibers.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Present in primary breast cancer tissue, absent from normal breast tissue.

Subunit Structure:

Homodimer. Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain.

Family&Domains:

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Belongs to the tropomyosin family.

Research Fields

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

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