HNF1A Antibody | Affinity Biosciences

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Product:	HNF1A Antibody
Catalog:	DF7180
Description:	Rabbit polyclonal antibody to HNF1A
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	56kDa; 67kD(Calculated).
Uniprot:	P20823
RRID:	AB_2839132

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

HNF1A Antibody detects endogenous levels of total HNF1A.

RRID:

AB_2839132
Cite Format: Affinity Biosciences Cat# DF7180, RRID:AB_2839132.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Albumin proximal factor; Hepatic nuclear factor 1 alpha; Hepatic nuclear factor 1; Hepatic transcription factor 1 alpha; Hepatic transcription factor 1; Hepatocyte nuclear factor 1-alpha; HNF 1; HNF 1A; HNF-1-alpha; HNF-1A; hnf1a; HNF1A_HUMAN; Interferon production regulator factor; LF B1; LF B1 hepatic nuclear factor; LFB 1; LFB1; LFB1 hepatic nuclear factor; Liver specific transcription factor LF B1; Liver specific transcription factor LFB1; Liver-specific transcription factor LF-B1; Maturity onset diabetes of the young 3; MODY 3; MODY3; TCF 1; TCF-1; TCF1; Transcription factor 1; Transcription factor 1 hepatic;

Immunogens

Immunogen:

Uniprot:

P20823(HNF1A_HUMAN) >>Visit HPA database.

Gene(ID):

HNF1A(6927)

Expression:

P20823 HNF1A_HUMAN:

Liver.

Description:

The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas.

Sequence:

P20823 HNF1A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVSKLSQLQTELLAALLESGLSKEALIQALGEPGPYLLAGEGPLDKGESCGGGRGELAELPNGLGETRGSEDETDDDGEDFTPPILKELENLSPEEAAHQKAVVETLLQEDPWRVAKMVKSYLQQHNIPQREVVDTTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREVAQQFTHAGQGGLIEEPTGDELPTKKGRRNRFKWGPASQQILFQAYERQKNPSKEERETLVEECNRAECIQRGVSPSQAQGLGSNLVTEVRVYNWFANRRKEEAFRHKLAMDTYSGPPPGPGPGPALPAHSSPGLPPPALSPSKVHGVRYGQPATSETAEVPSSSGGPLVTVSTPLHQVSPTGLEPSHSLLSTEAKLVSAAGGPLPPVSTLTALHSLEQTSPGLNQQPQNLIMASLPGVMTIGPGEPASLGPTFTNTGASTLVIGLASTQAQSVPVINSMGSSLTTLQPVQFSQPLHPSYQQPLMPPVQSHVTQSPFMATMAQLQSPHALYSHKPEVAQYTHTGLLPQTMLITDTTNLSALASLTPTKQVFTSDTEASSESGLHTPASQATTLHVPSQDPAGIQHLQPAHRLSASPTVSSSSLVLYQSSDSSNGQSHLLPSNHSVIETFISTQMASSSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Pig

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20823 As Substrate

P20823

Site	PTM Type	Enzyme	Source
T67	Phosphorylation		Uniprot
S70	Phosphorylation		Uniprot
T74	Phosphorylation		Uniprot
T82	Phosphorylation		Uniprot
S93	Phosphorylation		Uniprot
T196	Phosphorylation		Uniprot
S247	Phosphorylation		Uniprot
S249	Phosphorylation	Q9Y463 (DYRK1B) , Q13315 (ATM)	Uniprot

Research Backgrounds

P20823_HNF1A_HUMAN

Function:

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).

Subcellular Location:

Nucleus.

Tissue Specificity:

Liver.

Subunit Structure:

Binds DNA as a dimer. Heterotetramer with PCBD1; formed by a dimer of dimers (By similarity). Interacts with PCBD1. Interacts with BHLHE41 (By similarity).

Family&Domains:

Belongs to the HNF1 homeobox family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

References

1). SLC25A18 has prognostic value in colorectal cancer and represses Warburg effect and cell proliferation via Wnt signaling. American Journal of Cancer Research (PubMed: 32509397) [IF=5.3]

Application: WB Species: human Sample:

Figure 3.| SLC25A18 negatively regulates genes expression related to aerobics glycolysis, cell proliferation, andWnt/β-catenin cascade. (B) Gene set enrichment analysis implied a negative relationship between highly expressed SLC25A18 and the enrichment of CTNNB1 target genes and Wnt/β-catenin Signaling (HALLMARK_ WNT_BETA_CATENIN_SIGNALING, REACTOME_SIGNALING_BY_WNT) in patients with CRC from the TCGA database, (C)which was also verified by western blotting. All experiments were conducted in triplicates.

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HNF1A Antibody - #DF7180