Product: RAD50 Antibody
Catalog: DF7176
Description: Rabbit polyclonal antibody to RAD50
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 154kDa; 154kD(Calculated).
Uniprot: Q92878
RRID: AB_2839128

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(86%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
RAD50 Antibody detects endogenous levels of total RAD50.
RRID:
AB_2839128
Cite Format: Affinity Biosciences Cat# DF7176, RRID:AB_2839128.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA repair protein RAD50; hRAD50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog (S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50, S. cerevisiae, homolog of; RAD50_HUMAN; RAD502; Rad50l; Truncated RAD50 protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q92878 RAD50_HUMAN:

Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

Description:
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Sequence:
MSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKVAQETDVRAQIRLQFRDVNGELIAVQRSMVCTQKSKKTEFKTLEGVITRTKHGEKVSLSSKCAEIDREMISSLGVSKAVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALETLRQVRQTQGQKVKEYQMELKYLKQYKEKACEIRDQITSKEAQLTSSKEIVKSYENELDPLKNRLKEIEHNLSKIMKLDNEIKALDSRKKQMEKDNSELEEKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCHRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQEHIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEGEAKTANQLMNDFAEKETLKQKQIDEIRDKKTGLGRIIELKSEILSKKQNELKNVKYELQQLEGSSDRILELDQELIKAERELSKAEKNSNVETLKMEVISLQNEKADLDRTLRKLDQEMEQLNHHTTTRTQMEMLTKDKADKDEQIRKIKSRHSDELTSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASSEQNKNHINNELKRKEEQLSSYEDKLFDVCGSQDFESDLDRLKEEIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPVCQRVFQTEAELQEVISDLQSKLRLAPDKLKSTESELKKKEKRRDEMLGLVPMRQSIIDLKEKEIPELRNKLQNVNRDIQRLKNDIEEQETLLGTIMPEEESAKVCLTDVTIMERFQMELKDVERKIAQQAAKLQGIDLDRTVQQVNQEKQEKQHKLDTVSSKIELNRKLIQDQQEQIQHLKSTTNELKSEKLQISTNLQRRQQLEEQTVELSTEVQSLYREIKDAKEQVSPLETTLEKFQQEKEELINKKNTSNKIAQDKLNDIKEKVKNIHGYMKDIENYIQDGKDDYKKQKETELNKVIAQLSECEKHKEKINEDMRLMRQDIDTQKIQERWLQDNLTLRKRNEELKEVEEERKQHLKEMGQMQVLQMKSEHQKLEENIDNIKRNHNLALGRQKGYEEEIIHFKKELREPQFRDAEEKYREMMIVMRTTELVNKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENVSASDKRRNYNYRVVMLKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGIIALDEPTTNLDRENIESLAHALVEIIKSRSQQRNFQLLVITHDEDFVELLGRSEYVEKFYRIKKNIDQCSEIVKCSVSSLGFNVH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
86
Xenopus
86
Zebrafish
86
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92878 As Substrate

Site PTM Type Enzyme
K6 Ubiquitination
K20 Methylation
K62 Ubiquitination
K71 Ubiquitination
K112 Ubiquitination
K126 Ubiquitination
S128 Phosphorylation
S130 Phosphorylation
K132 Ubiquitination
K175 Ubiquitination
K187 Ubiquitination
R193 Methylation
T198 Phosphorylation
K211 Ubiquitination
K230 Ubiquitination
K238 Ubiquitination
K242 Ubiquitination
K252 Ubiquitination
K256 Ubiquitination
S263 Phosphorylation
K264 Acetylation
K273 Ubiquitination
K280 Ubiquitination
K295 Ubiquitination
Y308 Phosphorylation
K343 Ubiquitination
R359 Methylation
K398 Acetylation
K446 Ubiquitination
K453 Acetylation
K461 Acetylation
K461 Ubiquitination
S470 Phosphorylation
S471 Phosphorylation
R473 Methylation
K483 Ubiquitination
K520 Ubiquitination
K585 Acetylation
K599 Ubiquitination
K616 Ubiquitination
S623 Phosphorylation
S624 Phosphorylation
S635 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
S640 Phosphorylation
T690 Phosphorylation
K743 Ubiquitination
K753 Ubiquitination
R797 Methylation
R823 Methylation
K838 Ubiquitination
K845 Ubiquitination
K851 Ubiquitination
K864 Ubiquitination
K921 Ubiquitination
K959 Acetylation
Y964 Phosphorylation
K982 Ubiquitination
K1032 Acetylation
K1059 Ubiquitination
K1068 Ubiquitination
K1089 Ubiquitination
T1113 Phosphorylation
T1114 Phosphorylation
K1119 Ubiquitination
Y1125 Phosphorylation
K1126 Ubiquitination
K1134 Ubiquitination
Y1162 Phosphorylation
K1178 Ubiquitination
K1190 Ubiquitination
K1285 Acetylation
K1285 Ubiquitination
K1291 Ubiquitination
K1301 Ubiquitination

Research Backgrounds

Function:

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

Subcellular Location:

Nucleus. Chromosome>Telomere. Chromosome.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

Subunit Structure:

Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM8 and MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis. Interacts with MRNIP.

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

Family&Domains:

The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11 homodimer (By similarity).

Belongs to the SMC family. RAD50 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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