Product: HCRTR2 Antibody
Catalog: DF7162
Description: Rabbit polyclonal antibody to HCRTR2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 51kDa; 51kD(Calculated).
Uniprot: O43614
RRID: AB_2839114

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
HCRTR2 Antibody detects endogenous levels of total HCRTR2.
RRID:
AB_2839114
Cite Format: Affinity Biosciences Cat# DF7162, RRID:AB_2839114.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hcr tr2; Hcrt receptor 2; Hcrtr2; Hypocretin (orexin) receptor 2; Hypocretin receptor 2; Hypocretin receptor type 2; Orexin 2 receptor; Orexin A/Orexin B receptor; Orexin receptor 2; Orexin receptor type 2; Orexin type 2 receptor; Ox-2-R; Ox2-R; Ox2r; OX2R_HUMAN; Type 2 hypocretin receptor; Type 2 orexin receptor;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A.
Sequence:
MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHPKEYEWVLIAGYIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNSIVIIWIVSCIIMIPQAIVMECSTVFPGLANKTTLFTVCDERWGGEIYPKMYHICFFLVTYMAPLCLMVLAYLQIFRKLWCRQIPGTSSVVQRKWKPLQPVSQPRGPGQPTKSRMSAVAAEIKQIRARRKTARMLMIVLLVFAICYLPISILNVLKRVFGMFAHTEDRETVYAWFTFSHWLVYANSAANPIIYNFLSGKFREEFKAAFSCCCLGVHHRQEDRLTRGRTSTESRKSLTTQISNFDNISKLSEQVVLTSISTLPAANGAGPLQNW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43614 As Substrate

Site PTM Type Enzyme
Y223 Phosphorylation
T231 Phosphorylation
Y243 Phosphorylation
S273 Phosphorylation
Y317 Phosphorylation
S321 Phosphorylation

Research Backgrounds

Function:

Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

The N-terminal region is required for orexin signaling.

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). Soporific effect of modified Suanzaoren Decoction on mice models of insomnia by regulating Orexin-A and HPA axis homeostasis. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34509822) [IF=7.5]

Application: WB    Species: Mice    Sample: hypothalamus tissue

Fig. 9. Effect of MSZRD on the level of Orexin-A and the expression of OX2R. (a) The level of Orexin-A in serum was measured by ELISA kits. (b) OX2R protein was quantified as IOD/Area by Imagine Pro. (c) The expression of OX2R in hypothalamus was detected by western blot and (d) Statistical analysis on OX2R. (e) The expression of OX2R protein in hypothalamus tissue was examined by immunohistochemical staining (400×,brown-yellow granules indicate positive expression). Data are expressed as the mean ± SD. # P < 0.05, ## P < 0.01 compare with control group; *P < 0.05 and **P < 0.01 compare with model group.

Application: IHC    Species: Mice    Sample: hypothalamus tissue

Fig. 9. Effect of MSZRD on the level of Orexin-A and the expression of OX2R. (a) The level of Orexin-A in serum was measured by ELISA kits. (b) OX2R protein was quantified as IOD/Area by Imagine Pro. (c) The expression of OX2R in hypothalamus was detected by western blot and (d) Statistical analysis on OX2R. (e) The expression of OX2R protein in hypothalamus tissue was examined by immunohistochemical staining (400×,brown-yellow granules indicate positive expression). Data are expressed as the mean ± SD. # P < 0.05, ## P < 0.01 compare with control group; *P < 0.05 and **P < 0.01 compare with model group.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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