POMC Antibody - #DF7154

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Product: POMC Antibody
Catalog: DF7154
Description: Rabbit polyclonal antibody to POMC
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog, Chicken
Mol.Wt.: 29kDa; 29kD(Calculated).
Uniprot: P01189
RRID: AB_2839106

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Bovine(90%), Sheep(90%), Dog(90%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
POMC Antibody detects endogenous levels of total POMC.
RRID:
AB_2839106
Cite Format: Affinity Biosciences Cat# DF7154, RRID:AB_2839106.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACTH; Adrenocorticotropic hormone; Adrenocorticotropin; Alpha melanocyte stimulating hormone; alpha-MSH; alphaMSH; Beta LPH; Beta melanocyte stimulating hormone; Beta-endorphin; beta-MSH; CLIP; Corticotropin; Corticotropin lipotropin; Corticotropin-like intermediary peptide; Gamma LPH; gamma-MSH; Lipotropin beta; Lipotropin gamma; Lipotropin, included; LPH; Melanocyte-stimulating hormone, included; Melanotropin alpha; Melanotropin beta; Melanotropin gamma; Melanotropin, included; Met-enkephalin; MSH; NPP; Opiomelanocortin prepropeptide; POC; POMC; Pomc-1; Pomc1; Pomc2; Pro ACTH endorphin; Pro opiomelanocortin; Pro-opiomelanocortin-alpha; Proopiomelanocortin; Proopiomelanocortin preproprotein; Tetracosactide;

Immunogens

Immunogen:
Uniprot:

P01189(COLI_HUMAN) >>Visit HPA database.

Gene(ID):
POMC(5443)
Expression:
P01189 COLI_HUMAN:

ACTH and MSH are produced by the pituitary gland.

Description:
This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation.
Sequence:
MPRSCCSRSGALLLALLLQASMEVRGWCLESSQCQDLTTESNLLECIRACKPDLSAETPMFPGNGDEQPLTENPRKYVMGHFRWDRFGRRNSSSSGSSGAGQKREDVSAGEDCGPLPEGGPEPRSDGAKPGPREGKRSYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEFKRELTGQRLREGDGPDGPADDGAGAQADLEHSLLVAAEKKDEGPYRMEHFRWGSPPKDKRYGGFMTSEKSQTPLVTLFKNAIIKNAYKKGE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
90
Sheep
90
Dog
90
Pig
80
Chicken
80
Horse
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01189 As Substrate

Site PTM Type Enzyme
T71 O-Glycosylation
S92 Phosphorylation
S95 Phosphorylation
S97 Phosphorylation
S108 Phosphorylation
S168 Phosphorylation
S230 Phosphorylation

Research Backgrounds

Function:

Stimulates the adrenal glands to release cortisol.

Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.

Increases the pigmentation of skin by increasing melanin production in melanocytes.

Endogenous orexigenic opiate.

Endogenous opiate.

PTMs:

Specific enzymatic cleavages at paired basic residues yield the different active peptides.

O-glycosylated; reducing sugar is probably N-acetylgalactosamine.

Subcellular Location:

Secreted.
Note: Melanocyte-stimulating hormone alpha and beta-endorphin are stored in separate granules in hypothalamic POMC neurons, suggesting that secretion may be under the control of different regulatory mechanisms.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

ACTH and MSH are produced by the pituitary gland.

Family&Domains:

Belongs to the POMC family.

Research Fields

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

References

1). Metabolic regulation of Ganoderma lucidum extracts in high sugar and fat diet-induced obese mice by regulating the gut-brain axis. Journal of Functional Foods, 2020 [IF=5.6]

Application: WB    Species: Mouse    Sample: hypothalamus

Fig. 12. AGL regulates the leptin-mediated related signaling molecules. The expression of LEPR, AGPR, NPY, POMC and GAD65 in the hypothalamus assessed by western blotting. Data are presented as the means ± SD of more than 3 independent experiments. *p 
2). Chronic heat stress alters hypothalamus integrity, the serum indexes and attenuates expressions of hypothalamic appetite genes in broilers. Journal of Thermal Biology, 2019 (PubMed: 30975407) [IF=2.7]

Application: IF/ICC    Species: broilers    Sample: hypothalamus

Fig. 6.| Effect of chronic heat stress on protein levels of POMC in the hypothalamus of broilers after 14 days heat stress based on the whole-brain immunofluorescent method of the control group (A), heat stress group (B), pair-fed group (C). POMC, proopiomelanocortin; White arrows on the right panels indicates POMC proteins which colors are red. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).

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