ABCC1 Antibody | Affinity Biosciences

WB
IHC
Cites

1/4

Western blot analysis of extracts from Rat liver, using ABCC1 Antibody.

DF7148 at 1/100 staining Rat testis tissue by IHC-P.

FIGURE 3 miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR.

FIGURE 5 The expressions of drug‐resistant‐related proteins (ABCC1) in hippocampus of DRE rats at 6, 7, and 9 weeks after SE.

Product:	ABCC1 Antibody
Catalog:	DF7148
Description:	Rabbit polyclonal antibody to ABCC1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish
Mol.Wt.:	173kDa; 172kD(Calculated).
Uniprot:	P33527
RRID:	AB_2839101

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(92%)

Clonality:

Polyclonal

Specificity:

ABCC1 Antibody detects endogenous levels of total ABCC1.

RRID:

AB_2839101
Cite Format: Affinity Biosciences Cat# DF7148, RRID:AB_2839101.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ABC 29; ABC29; ABCC 1; ABCC; Abcc1; ATP binding cassette sub family C (CFTR/MRP) member 1; ATP binding cassette sub-family C member 1; ATP binding cassette subfamily C member 1; ATP binding cassette transporter variant ABCC1delta ex13; ATP binding cassette transporter variant ABCC1delta ex13&14; ATP binding cassette transporter variant ABCC1delta ex25; ATP binding cassette transporter variant ABCC1delta ex25&26; ATP binding cassette, sub-family C (CFTR/MRP), member 1; ATP-binding cassette sub-family C member 1; DKFZp686N04233; DKFZp781G125; GS X; GSX; Leukotriene C(4) transporter; LTC4 transporter; MRP 1; MRP; MRP1; MRP1_HUMAN; Multidrug resistance associated protein 1; Multidrug resistance protein; Multidrug resistance-associated protein 1; Multiple drug resistance associated protein; Multiple drug resistance protein 1;

Immunogens

Immunogen:

Uniprot:

P33527(MRP1_HUMAN) >>Visit HPA database.

Gene(ID):

ABCC1(4363)

Expression:

P33527 MRP1_HUMAN:

Lung, testis and peripheral blood mononuclear cells.

Description:

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.

Sequence:

P33527 MRP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALRGFCSADGSDPLWDWNVTWNTSNPDFTKCFQNTVLVWVPCFYLWACFPFYFLYLSRHDRGYIQMTPLNKTKTALGFLLWIVCWADLFYSFWERSRGIFLAPVFLVSPTLLGITMLLATFLIQLERRKGVQSSGIMLTFWLVALVCALAILRSKIMTALKEDAQVDLFRDITFYVYFSLLLIQLVLSCFSDRSPLFSETIHDPNPCPESSASFLSRITFWWITGLIVRGYRQPLEGSDLWSLNKEDTSEQVVPVLVKNWKKECAKTRKQPVKVVYSSKDPAQPKESSKVDANEEVEALIVKSPQKEWNPSLFKVLYKTFGPYFLMSFFFKAIHDLMMFSGPQILKLLIKFVNDTKAPDWQGYFYTVLLFVTACLQTLVLHQYFHICFVSGMRIKTAVIGAVYRKALVITNSARKSSTVGEIVNLMSVDAQRFMDLATYINMIWSAPLQVILALYLLWLNLGPSVLAGVAVMVLMVPVNAVMAMKTKTYQVAHMKSKDNRIKLMNEILNGIKVLKLYAWELAFKDKVLAIRQEELKVLKKSAYLSAVGTFTWVCTPFLVALCTFAVYVTIDENNILDAQTAFVSLALFNILRFPLNILPMVISSIVQASVSLKRLRIFLSHEELEPDSIERRPVKDGGGTNSITVRNATFTWARSDPPTLNGITFSIPEGALVAVVGQVGCGKSSLLSALLAEMDKVEGHVAIKGSVAYVPQQAWIQNDSLRENILFGCQLEEPYYRSVIQACALLPDLEILPSGDRTEIGEKGVNLSGGQKQRVSLARAVYSNADIYLFDDPLSAVDAHVGKHIFENVIGPKGMLKNKTRILVTHSMSYLPQVDVIIVMSGGKISEMGSYQELLARDGAFAEFLRTYASTEQEQDAEENGVTGVSGPGKEAKQMENGMLVTDSAGKQLQRQLSSSSSYSGDISRHHNSTAELQKAEAKKEETWKLMEADKAQTGQVKLSVYWDYMKAIGLFISFLSIFLFMCNHVSALASNYWLSLWTDDPIVNGTQEHTKVRLSVYGALGISQGIAVFGYSMAVSIGGILASRCLHVDLLHSILRSPMSFFERTPSGNLVNRFSKELDTVDSMIPEVIKMFMGSLFNVIGACIVILLATPIAAIIIPPLGLIYFFVQRFYVASSRQLKRLESVSRSPVYSHFNETLLGVSVIRAFEEQERFIHQSDLKVDENQKAYYPSIVANRWLAVRLECVGNCIVLFAALFAVISRHSLSAGLVGLSVSYSLQVTTYLNWLVRMSSEMETNIVAVERLKEYSETEKEAPWQIQETAPPSSWPQVGRVEFRNYCLRYREDLDFVLRHINVTINGGEKVGIVGRTGAGKSSLTLGLFRINESAEGEIIIDGINIAKIGLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSLELAHLKDFVSALPDKLDHECAEGGENLSVGQRQLVCLARALLRKTKILVLDEATAAVDLETDDLIQSTIRTQFEDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEYGAPSDLLQQRGLFYSMAKDAGLV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Zebrafish

Bovine

Dog

Chicken

Xenopus

Pig

Horse

Sheep

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P33527 As Substrate

P33527

Site	PTM Type	Enzyme	Source
N19	N-Glycosylation		Uniprot
N23	N-Glycosylation		Uniprot
K72	Ubiquitination		Uniprot
K246	Ubiquitination		Uniprot
T249	Phosphorylation	P68400 (CSNK2A1)	Uniprot
K259	Ubiquitination		Uniprot
K274	Ubiquitination		Uniprot
Y277	Phosphorylation		Uniprot
S279	Phosphorylation		Uniprot
K280	Ubiquitination		Uniprot
S289	Phosphorylation		Uniprot
K290	Ubiquitination		Uniprot
K303	Ubiquitination		Uniprot
K307	Ubiquitination		Uniprot
K315	Ubiquitination		Uniprot
K396	Ubiquitination		Uniprot
Y404	Phosphorylation		Uniprot
K406	Ubiquitination		Uniprot
K416	Ubiquitination		Uniprot
K488	Ubiquitination		Uniprot
K496	Ubiquitination		Uniprot
S497	Phosphorylation		Uniprot
K498	Acetylation		Uniprot
K498	Ubiquitination		Uniprot
K503	Acetylation		Uniprot
K503	Ubiquitination		Uniprot
K513	Ubiquitination		Uniprot
K525	Ubiquitination		Uniprot
K527	Ubiquitination		Uniprot
K636	Ubiquitination		Uniprot
K764	Ubiquitination		Uniprot
K773	Ubiquitination		Uniprot
K804	Ubiquitination		Uniprot
K814	Ubiquitination		Uniprot
K818	Ubiquitination		Uniprot
S847	Phosphorylation		Uniprot
Y852	Phosphorylation		Uniprot
S871	Phosphorylation		Uniprot
T872	Phosphorylation		Uniprot
K891	Ubiquitination		Uniprot
K894	Ubiquitination		Uniprot
S905	Phosphorylation		Uniprot
K908	Ubiquitination		Uniprot
S915	Phosphorylation		Uniprot
S916	Phosphorylation		Uniprot
S917	Phosphorylation		Uniprot
S918	Phosphorylation		Uniprot
S919	Phosphorylation		Uniprot
Y920	Phosphorylation		Uniprot
S921	Phosphorylation		Uniprot
S925	Phosphorylation		Uniprot
S930	Phosphorylation		Uniprot
T931	Phosphorylation		Uniprot
K936	Ubiquitination		Uniprot
K946	Ubiquitination		Uniprot
K952	Ubiquitination		Uniprot
S961	Phosphorylation		Uniprot
N1006	N-Glycosylation		Uniprot
K1078	Ubiquitination		Uniprot
S1145	Phosphorylation		Uniprot
K1181	Ubiquitination		Uniprot
K1187	Ubiquitination		Uniprot
Y1243	Phosphorylation		Uniprot
K1265	Ubiquitination		Uniprot
K1272	Ubiquitination		Uniprot
K1322	Ubiquitination		Uniprot
K1333	Ubiquitination		Uniprot
K1360	Ubiquitination		Uniprot
K1369	Ubiquitination		Uniprot
K1502	Ubiquitination		Uniprot
Y1508	Phosphorylation		Uniprot
Y1522	Phosphorylation		Uniprot
S1523	Phosphorylation		Uniprot
K1526	Ubiquitination		Uniprot

Research Backgrounds

P33527_MRP1_HUMAN

Function:

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. Hydrolyzes ATP with low efficiency. Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation. Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Lung, testis and peripheral blood mononuclear cells.

Family&Domains:

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Digestive system > Vitamin digestion and absorption.

References

1). 4′-OH as the Action Site of Lipids and MRP1 for Enhanced Transdermal Delivery of Flavonoids. ACS Applied Materials & Interfaces, 2023 (PubMed: 36913526) [IF=9.5]

2). miR-506 enhances the sensitivity of human colorectal cancer cells to oxaliplatin by suppressing MDR1/P-gp expression. CELL PROLIFERATION, 2017 (PubMed: 28217977) [IF=8.5]

Application: WB Species: human Sample:

FIGURE 3 miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR. A, The mRNA level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant genes as demonstrated by qRT-PCR. B, The protein level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant proteins as demonstrated by Western blot. C, Expression of P-gp detected by immunofluorescence staining.HCT116-OxR-miR-506 cells showed low levels of fluorescent staining of P-gp, whereas maximal staining of P-gp was observed in HCT116-OxR cells, readily distinguished from background. Zoom: 200×. *P<.05)

3). miR‐485's anti‐drug resistant epilepsy effects by regulating SV2A/PSD‐95 and targeting ABCC1 and neuronal signaling‐transduction proteins in hippocampus of rats. Brain and Behavior, 2021 (PubMed: 34291586) [IF=3.1]

Application: WB Species: Rat Sample: hippocampus

FIGURE 5 The expressions of drug‐resistant‐related proteins (ABCC1) in hippocampus of DRE rats at 6, 7, and 9 weeks after SE. *p < .05 versus the control group; # p < .5 versus the epilepsy group

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ABCC1 Antibody - #DF7148