LRPAP1 Antibody - #DF7141

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Product: LRPAP1 Antibody
Catalog: DF7141
Description: Rabbit polyclonal antibody to LRPAP1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 41kDa; 41kD(Calculated).
Uniprot: P30533
RRID: AB_2839095

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(89%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
LRPAP1 Antibody detects endogenous levels of total LRPAP1.
RRID:
AB_2839095
Cite Format: Affinity Biosciences Cat# DF7141, RRID:AB_2839095.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

39 kDa receptor-associated protein; A2MRAP; A2RAP; Alpha 2 macroglobulin receptor associated protein; Alpha 2 MRAP; Alpha-2-macroglobulin receptor-associated protein; Alpha-2-MRAP; AMRP_HUMAN; HBP44; Lipoprotein receptor associated protein; Low density lipoprotein receptor related protein associated protein 1; Low density lipoprotein receptor-related protein-associated protein 1; Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein; Low density lipoprotein related protein associated protein 1; low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1); Lrpap1; MGC138272; MRAP; MYP23; RAP;

Immunogens

Immunogen:
Uniprot:

P30533(AMRP_HUMAN) >>Visit HPA database.

Gene(ID):
LRPAP1(4043)
Description:
Low density lipoprotein receptor-related protein associated protein 1 also known as LRPAP1 or RAP is a chaperone protein which in humans is encoded by the LRPAP1 gene. LRPAP1 is involved with trafficking of certain members of the LDL receptor family including LRP1 and LRP2. It is a glycoprotein that binds to the alpha-2-macroglobulin receptor, as well as to other members of the low density lipoprotein receptor family. It acts to inhibit the binding of all know ligands for these receptors, and may prevent receptor aggregation and degradation in the endoplasmic reticulum, thereby acting as a molecular chaperone. It may be under the regulatory control of calmodulin, since it is able to bind calmodulin and be phosphorylated by calmodulin-dependent kinase II.
Sequence:
MAPRRVRSFLRGLPALLLLLLFLGPWPAASHGGKYSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Chicken
92
Sheep
89
Pig
75
Zebrafish
67
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30533 As Substrate

Site PTM Type Enzyme
S45 Phosphorylation
S50 Phosphorylation
T134 O-Glycosylation
S135 Phosphorylation
S137 Phosphorylation
T141 Phosphorylation
K153 Ubiquitination
S165 Phosphorylation
Y186 Phosphorylation
S230 Phosphorylation
S242 Phosphorylation
S247 Phosphorylation
T248 Phosphorylation
N268 N-Glycosylation
K287 Acetylation
K304 Ubiquitination
S310 Phosphorylation
K332 Ubiquitination
Y336 Phosphorylation
T337 O-Glycosylation
S346 O-Glycosylation
R348 Methylation

Research Backgrounds

Function:

Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.

PTMs:

N-glycosylated.

Subcellular Location:

Rough endoplasmic reticulum lumen. Endoplasmic reticulum-Golgi intermediate compartment lumen. Golgi apparatus>cis-Golgi network. Golgi apparatus lumen. Endosome lumen. Cell surface.
Note: May be associated with receptors at the cell surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with the LRP1/alpha-2-macroglobulin receptor heavy and light chains; the interaction is transient and coincides with a reduction of ligand binding by the receptor. Interacts with LRP2/glycoprotein 330. Interacts with LRP1B; binding is followed by internalization and degradation. Interacts with LDLR. Interacts with SORL1. Interacts with LRP1; this interaction is followed by rapid internalization.

Family&Domains:

Belongs to the alpha-2-MRAP family.

Research Fields

· Organismal Systems > Digestive system > Cholesterol metabolism.

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