KISS1R Antibody - #DF7123

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Product: KISS1R Antibody
Catalog: DF7123
Description: Rabbit polyclonal antibody to KISS1R
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 43kDa; 43kD(Calculated).
Uniprot: Q969F8
RRID: AB_2839077

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
KISS1R Antibody detects endogenous levels of total KISS1R.
RRID:
AB_2839077
Cite Format: Affinity Biosciences Cat# DF7123, RRID:AB_2839077.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AXOR 12; AXOR12; G protein coupled receptor 54; G-protein coupled receptor 54; G-protein coupled receptor OT7T175; GPCR 54; GPCR54; GPR 54; GPR54; hOT7T175; Hypogonadotropin 1; Hypogonadotropin-1; Hypogonadotropin1; KISS 1 receptor; KISS 1R; KiSS-1 receptor; KiSS-1R; KISS1 receptor; Kiss1r; Kisspeptins receptor; KISSR_HUMAN; Metastin receptor; OT7T175;

Immunogens

Immunogen:
Uniprot:

Q969F8(KISSR_HUMAN) >>Visit HPA database.

Gene(ID):
KISS1R(84634)
Expression:
Q969F8 KISSR_HUMAN:

Most highly expressed in the pancreas, placenta and spinal cord, with lower-level of expression in peripheral blood leukocytes, kidney, lung, fetal liver, stomach, small intestine, testes, spleen, thymus, adrenal glands and lymph nodes. In the adult brain, expressed in the superior frontal gyrus, putamen, caudate nucleus, cingulate gyrus, nucleus accumbens, hippocampus, pons and amygdala, as well as the hypothalamus and pituitary. Expression levels are higher in early (7-9 weeks) than term placentas. Expression levels were increased in both early placentas and molar pregnancies and were reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation. Also found in the extravillous trophoblast suggesting endocrine/paracrine activation mechanism.

Description:
The protein encoded by this gene is a galanin-like G protein-coupled receptor that binds metastin, a peptide encoded by the metastasis suppressor gene KISS1. The tissue distribution of the expressed gene suggests that it is involved in the regulation of endocrine function, and this is supported by the finding that this gene appears to play a role in the onset of puberty. Mutations in this gene have been associated with hypogonadotropic hypogonadism and central precocious puberty.
Sequence:
MHTVATSGPNASWGAPANASGCPGCGANASDGPVPSPRAVDAWLVPLFFAALMLLGLVGNSLVIYVICRHKPMRTVTNFYIANLAATDVTFLLCCVPFTALLYPLPGWVLGDFMCKFVNYIQQVSVQATCATLTAMSVDRWYVTVFPLRALHRRTPRLALAVSLSIWVGSAAVSAPVLALHRLSPGPRAYCSEAFPSRALERAFALYNLLALYLLPLLATCACYAAMLRHLGRVAVRPAPADSALQGQVLAERAGAVRAKVSRLVAAVVLLFAACWGPIQLFLVLQALGPAGSWHPRSYAAYALKTWAHCMSYSNSALNPLLYAFLGSHFRQAFRRVCPCAPRRPRRPRRPGPSDPAAPHAELLRLGSHPAPARAQKPGSSGLAARGLCVLGEDNAPL

PTMs - Q969F8 As Substrate

Site PTM Type Enzyme
S368 Phosphorylation

Research Backgrounds

Function:

Receptor for metastin (kisspeptin-54 or kp-54), a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Most highly expressed in the pancreas, placenta and spinal cord, with lower-level of expression in peripheral blood leukocytes, kidney, lung, fetal liver, stomach, small intestine, testes, spleen, thymus, adrenal glands and lymph nodes. In the adult brain, expressed in the superior frontal gyrus, putamen, caudate nucleus, cingulate gyrus, nucleus accumbens, hippocampus, pons and amygdala, as well as the hypothalamus and pituitary. Expression levels are higher in early (7-9 weeks) than term placentas. Expression levels were increased in both early placentas and molar pregnancies and were reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation. Also found in the extravillous trophoblast suggesting endocrine/paracrine activation mechanism.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). Neuroendocrine Regulation of Stress-Induced T Cell Dysfunction during Lung Cancer Immunosurveillance via the Kisspeptin/GPR54 Signaling Pathway. Advanced Science [IF=15.1]
2). Impact of Physiologically Relevant Genistein Exposure at Different Time Windows on Puberty Onset and Neuroendocrine Function in Female Rats. Molecular Nutrition & Food Research (PubMed: 36106654) [IF=5.2]
3). Roles of the kisspeptin/GPR54 system in pathomechanisms of atherosclerosis. Nutrition, Metabolism and Cardiovascular Diseases (PubMed: 32409274) [IF=3.9]

Application: IHC    Species: mouse    Sample: atheromatous plaques

Figure 3| Expression of KP-10 and GPR54 within atheromatous plaques in Apoe/- mice. Apoe/- mice were fed a high-cholesterol diet from 13 weeks old. Aortic root walls from 13-, 17-, and 21-week-old Apoe/- mice were stained with Oil Red O (AeC), anti-KP-10 antibody(Bioss, bs-0749, 1:200) (DeF), or anti-GPR54 antibody (Affinity Biosciences, DF2751, 1:100) (GeI). Nuclei were stained with hematoxylin.Scale bar Z 200 mm. Atheromatous plaques (reddish areas stained by Oil Red O) became greater with age in Apoe/- mice. Likewise, expressions of KP-10 and GPR54 (brown) became greater within atheromatous plaques in Apoe/- mice. These results are unpublished data from our new experiments.

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