GRK1 Antibody - #DF7122

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Product: GRK1 Antibody
Catalog: DF7122
Description: Rabbit polyclonal antibody to GRK1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 63.5kDa; 64kD(Calculated).
Uniprot: Q15835
RRID: AB_2839076

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(88%), Horse(88%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GRK1 Antibody detects endogenous levels of total GRK1.
RRID:
AB_2839076
Cite Format: Affinity Biosciences Cat# DF7122, RRID:AB_2839076.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC=2.7.11.14; G protein-coupled receptor kinase 1; GPRK1; Grk1; Rhodopsin kinase; RHOK; RK; RK_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q15835(GRK1_HUMAN) >>Visit HPA database.

Gene(ID):
GRK1(6011)
Expression:
Q15835 GRK1_HUMAN:

Retinal-specific. Expressed in rods and cones cells.

Description:
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
Sequence:
MDFGSLETVVANSAFIAARGSFDGSSSQPSRDKKYLAKLKLPPLSKCESLRDSLSLEFESVCLEQPIGKKLFQQFLQSAEKHLPALELWKDIEDYDTADNDLQPQKAQTILAQYLDPQAKLFCSFLDEGIVAKFKEGPVEIQDGLFQPLLQATLAHLGQAPFQEYLGSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGKGGFGEVSACQMKATGKLYACKKLNKKRLKKRKGYQGAMVEKKILMKVHSRFIVSLAYAFETKADLCLVMTIMNGGDIRYHIYNVNEENPGFPEPRALFYTAQIICGLEHLHQRRIVYRDLKPENVLLDNDGNVRISDLGLAVELLDGQSKTKGYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFSQASKDFCEALLEKDPEKRLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAFSTVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMCLVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Xenopus
100
Rabbit
100
Horse
88
Bovine
88
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15835 As Substrate

Site PTM Type Enzyme
S21 Phosphorylation Q15835 (GRK1) , P17612 (PRKACA)
S78 Phosphorylation
K81 Acetylation
K418 Acetylation
K424 Acetylation
K437 Acetylation
S491 Phosphorylation Q15835 (GRK1)
T492 Phosphorylation Q15835 (GRK1)

PTMs - Q15835 As Enzyme

Substrate Site Source
P08100 (RHO) S334 Uniprot
P08100 (RHO) S338 Uniprot
P08100 (RHO) S343 Uniprot
P41145 (OPRK1) S358 Uniprot
Q15835 (GRK1) S21 Uniprot
Q15835 (GRK1) S491 Uniprot
Q15835 (GRK1) T492 Uniprot
Q5NUL3-2 (FFAR4) T347 Uniprot
Q5NUL3-2 (FFAR4) S350 Uniprot
Q5NUL3-2 (FFAR4) S357 Uniprot

Research Backgrounds

Function:

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).

PTMs:

Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.

Farnesylation is required for full activity.

Subcellular Location:

Membrane>Lipid-anchor. Cell projection>Cilium>Photoreceptor outer segment.
Note: Subcellular location is not affected by light or dark conditions.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Retinal-specific. Expressed in rods and cones cells.

Subunit Structure:

Interacts (via N-terminus) with RCVRN (via C-terminus); the interaction is Ca(2+)-dependent (By similarity). Interacts (when prenylated) with PDE6D; this promotes release from membranes (By similarity). May form a complex composed of RHO, GRK1 and RCVRN in a Ca(2+)-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Sensory system > Phototransduction.

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