GRM1 Antibody | Affinity Biosciences

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Product:	GRM1 Antibody
Catalog:	DF7120
Description:	Rabbit polyclonal antibody to GRM1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Dog
Mol.Wt.:	132kDa; 132kD(Calculated).
Uniprot:	Q13255
RRID:	AB_2839074

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

GRM1 Antibody detects endogenous levels of total GRM1.

RRID:

AB_2839074
Cite Format: Affinity Biosciences Cat# DF7120, RRID:AB_2839074.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Glutamate receptor metabotropic 1; glutamate receptor, metabotropic 1; GPRC1A; GRM1; GRM1-Alpha; GRM1_HUMAN; GRM1A; Metabotropic glutamate receptor 1; mGlu1; mGluR1; MGLUR1-Alpha; MGLUR1A; SCAR13;

Immunogens

Immunogen:

Uniprot:

Q13255(GRM1_HUMAN) >>Visit HPA database.

Gene(ID):

GRM1(2911)

Expression:

Q13255 GRM1_HUMAN:

Detected in brain.

Description:

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.

Sequence:

Q13255 GRM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVGLLLFFFPAIFLEVSLLPRSPGRKVLLAGASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGEIREQYGIQRVEAMFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGINRCLPDGQSLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYFLRVVPSDTLQARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKIYSNAGEKSFDRLLRKLRERLPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWADRDEVIEGYEVEANGGITIKLQSPEVRSFDDYFLKLRLDTNTRNPWFPEFWQHRFQCRLPGHLLENPNFKRICTGNESLEENYVQDSKMGFVINAIYAMAHGLQNMHHALCPGHVGLCDAMKPIDGSKLLDFLIKSSFIGVSGEEVWFDEKGDAPGRYDIMNLQYTEANRYDYVHVGTWHEGVLNIDDYKIQMNKSGVVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEYVQDEFTCKACDLGWWPNADLTGCEPIPVRYLEWSNIESIIAIAFSCLGILVTLFVTLIFVLYRDTPVVKSSSRELCYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNRIARILAGSKKKICTRKPRFMSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKEVYLICNTSNLGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIAKPERNVRSAFTTSDVVRMHVGDGKLPCRSNTFLNIFRRKKAGAGNANSNGKSVSWSEPGGGQVPKGQHMWHRLSVHVKTNETACNQTAVIKPLTKSYQGSGKSLTFSDTSTKTLYNVEEEEDAQPIRFSPPGSPSMVVHRRVPSAATTPPLPSHLTAEETPLFLAEPALPKGLPPPLQQQQQPPPQQKSLMDQLQGVVSNFSTAIPDFHAVLAGPGGPGNGLRSLYPPPPPPQHLQMLPLQLSTFGEELVSPPADDDDDSERFKLLQEYVYEHEREGNTEEDELEEEEEDLQAASKLTPDDSPALTPPSPFRDSVASGSSVPSSPVSESVLCTPPNVSYASVILRDYKQSSSTL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Bovine

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13255 As Substrate

Q13255

Site	PTM Type	Enzyme	Source
T188	Phosphorylation		Uniprot
T620	Phosphorylation		Uniprot
Y632	Phosphorylation		Uniprot
Y672	Phosphorylation		Uniprot
S673	Phosphorylation		Uniprot
T677	Phosphorylation		Uniprot
T695	Phosphorylation	P17252 (PRKCA)	Uniprot
K740	Methylation		Uniprot
S869	Phosphorylation		Uniprot
T871	Phosphorylation		Uniprot
K891	Ubiquitination		Uniprot
Y1109	Phosphorylation		Uniprot
Y1111	Phosphorylation		Uniprot

Research Backgrounds

Q13255_GRM1_HUMAN

Function:

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. May function in the light response in the retina (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Detected in brain.

Subunit Structure:

Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP (By similarity).

Family&Domains:

Belongs to the G-protein coupled receptor 3 family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

References

1). SIRT1 Activation Attenuates Bone Cancer Pain by Inhibiting mGluR1/5. CELLULAR AND MOLECULAR NEUROBIOLOGY (PubMed: 31270711) [IF=4.0]

Application: WB Species: mouse Sample: spinal cord

Fig. 4| Efect of intrathecal injections of SRT1720 on the levels of the mGluR1/5 protein and mRNA in the spinal cord of mice with BCP. a, b Western blots showing the levels of the mGluR1 protein. c, d Western blots showing the levels of the mGluR5 protein.

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For Research Use Only.
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GRM1 Antibody - #DF7120