EDNRB Antibody - #DF7104

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Product: EDNRB Antibody
Catalog: DF7104
Description: Rabbit polyclonal antibody to EDNRB
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 50kDa; 50kD(Calculated).
Uniprot: P24530
RRID: AB_2839059

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
EDNRB Antibody detects endogenous levels of total EDNRB.
RRID:
AB_2839059
Cite Format: Affinity Biosciences Cat# DF7104, RRID:AB_2839059.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABCDS; Ednra; EDNRB; EDNRB_HUMAN; Endothelin B receptor; Endothelin B receptor precursor; Endothelin receptor Non selective type; Endothelin receptor non-selective type; Endothelin receptor type B; ET B; ET-B; ET-BR; ETB; ETBR; ETRB; Hirschsprung disease 2; HSCR; HSCR2; OTTHUMP00000018534; OTTHUMP00000178736; WS4A;

Immunogens

Immunogen:
Uniprot:

P24530(EDNRB_HUMAN) >>Visit HPA database.

Gene(ID):
EDNRB(1910)
Expression:
P24530 EDNRB_HUMAN:

Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.

Description:
The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Sequence:
MQPPPSLCGRALVALVLACGLSRIWGEERGFPPDRATPLLQTAEIMTPPTKTLWPKGSNASLARSLAPAEVPKGDRTAGSPPRTISPPPCQGPIEIKETFKYINTVVSCLVFVLGIIGNSTLLRIIYKNKCMRNGPNILIASLALGDLLHIVIDIPINVYKLLAEDWPFGAEMCKLVPFIQKASVGITVLSLCALSIDRYRAVASWSRIKGIGVPKWTAVEIVLIWVVSVVLAVPEAIGFDIITMDYKGSYLRICLLHPVQKTAFMQFYKTAKDWWLFSFYFCLPLAITAFFYTLMTCEMLRKKSGMQIALNDHLKQRREVAKTVFCLVLVFALCWLPLHLSRILKLTLYNQNDPNRCELLSFLLVLDYIGINMASLNSCINPIALYLVSKRFKNCFKSCLCCWCQSFEEKQSLEEKQSCLKFKANDHGYDNFRSSNKYSSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
100
Rabbit
100
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24530 As Substrate

Site PTM Type Enzyme
T52 Phosphorylation
S80 Phosphorylation
Y200 Phosphorylation
S205 Phosphorylation
S305 Phosphorylation
S407 Phosphorylation
S419 Phosphorylation
Y430 Phosphorylation
S435 Phosphorylation
S436 Phosphorylation
Y439 Phosphorylation
S440 Phosphorylation
S441 Phosphorylation
S442 Phosphorylation

Research Backgrounds

Function:

Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

PTMs:

Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: internalized after activation by endothelins.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

References

1). Deleterious effect in endothelin receptor–mediated coronary artery smooth muscle contractility in high-salt diet rats. Nutrition, Metabolism and Cardiovascular Diseases (PubMed: 36404239) [IF=3.9]

Application: WB    Species: Rat    Sample:

Figure 4Expression levels of ETA, ETB, STIM1, Orai1 in coronary arteries derived from 4% high-salt diet rats vs. controls. Western blot images (A–D) and summary data (E–H) of endothelial receptors ETA and ETB as well as of STIM1 and Orai1 in fresh isolated coronary arteries derived from rats fed a regular diet (control) and rats fed a high-salt diet. Protein expression levels were normalized to β-tubulin. Immunostaining results of ETA, ETB in segments of coronary arteries derived from control (I (a–b)) and high-salt diet (I (d–e)) rats (three rats in each group). The values are shown as mean ± SEM; n = 3–7 rats. ∗P < 0.05, ∗∗P < 0.01 compared with controls.

Application: IHC    Species: Rat    Sample:

Figure 4Expression levels of ETA, ETB, STIM1, Orai1 in coronary arteries derived from 4% high-salt diet rats vs. controls. Western blot images (A–D) and summary data (E–H) of endothelial receptors ETA and ETB as well as of STIM1 and Orai1 in fresh isolated coronary arteries derived from rats fed a regular diet (control) and rats fed a high-salt diet. Protein expression levels were normalized to β-tubulin. Immunostaining results of ETA, ETB in segments of coronary arteries derived from control (I (a–b)) and high-salt diet (I (d–e)) rats (three rats in each group). The values are shown as mean ± SEM; n = 3–7 rats. ∗P < 0.05, ∗∗P < 0.01 compared with controls.
2). Endothelin-1 and ET receptors impair left ventricular function by mediated coronary arteries dysfunction in chronic intermittent hypoxia rats. Physiological Reports (PubMed: 28057852) [IF=2.5]

Application: IHC    Species: rat    Sample: heart

Figure 7. |Coronary arteries expression and localization of ET-1 and ET-1 receptors in heart tissues from normoxia and CIH rats (magnification 4009). The dark brown color represents positive staining for ET-1 and ET-1 receptors. (A–F) Immunostaining of histological sections from normoxia (A, C, E) and CIH (B, D, F) rats for ET-1, ETA and ETB.

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