LAMP3 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from HT-29 , using LAMP3antibody.

Product:	LAMP3 Antibody
Catalog:	DF7099
Description:	Rabbit polyclonal antibody to LAMP3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	44kDa; 44kD(Calculated).
Uniprot:	Q9UQV4
RRID:	AB_2839054

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

LAMP3 Antibody detects endogenous levels of total LAMP3.

RRID:

AB_2839054
Cite Format: Affinity Biosciences Cat# DF7099, RRID:AB_2839054.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD208; CD208 antigen; DC LAMP; DC lysosome associated membrane glycoprotein; DC-lysosome-associated membrane glycoprotein; DCLAMP; LAMP; LAMP 3; LAMP-3; Lamp3; LAMP3_HUMAN; Lysosomal associated membrane protein 3; Lysosomal-associated membrane protein 3; Lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403;

Immunogens

Immunogen:

Uniprot:

Q9UQV4(LAMP3_HUMAN) >>Visit HPA database.

Gene(ID):

LAMP3(27074)

Expression:

Q9UQV4 LAMP3_HUMAN:

Detected in tonsil interdigitating dendritic cells, in spleen, lymph node, Peyer's patches in the small instestine, in thymus medulla and in B-cells (at protein level). Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.

Description:

Lysosome-associated membrane glycoprotein 3 is a protein that in humans is encoded by the LAMP3 gene. LAMP3 has also recently been designated CD208. Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses .May play a role in dendritic cell function and in adaptive immunity.

Sequence:

Q9UQV4 LAMP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPRQLSAAAALFASLAVILHDGSQMRAKAFPETRDYSQPTAAATVQDIKKPVQQPAKQAPHQTLAARFMDGHITFQTAATVKIPTTTPATTKNTATTSPITYTLVTTQATPNNSHTAPPVTEVTVGPSLAPYSLPPTITPPAHTTGTSSSTVSHTTGNTTQPSNQTTLPATLSIALHKSTTGQKPVQPTHAPGTTAAAHNTTRTAAPASTVPGPTLAPQPSSVKTGIYQVLNGSRLCIKAEMGIQLIVQDKESVFSPRRYFNIDPNATQASGNCGTRKSNLLLNFQGGFVNLTFTKDEESYYISEVGAYLTVSDPETIYQGIKHAVVMFQTAVGHSFKCVSEQSLQLSAHLQVKTTDVQLQAFDFEDDHFGNVDECSSDYTIVLPVIGAIVVGLCLMGMGVYKIRLRCQSSGYQRI

PTMs - Q9UQV4 As Substrate

Q9UQV4

Site	PTM Type	Source
S23	Phosphorylation	Uniprot
S37	O-Glycosylation	Uniprot
T40	O-Glycosylation	Uniprot
T44	O-Glycosylation	Uniprot
T74	O-Glycosylation	Uniprot
T77	O-Glycosylation	Uniprot
T80	O-Glycosylation	Uniprot
S234	Phosphorylation	Uniprot
Y260	Phosphorylation	Uniprot
T268	Phosphorylation	Uniprot
S271	Phosphorylation	Uniprot
T276	Phosphorylation	Uniprot
N291	N-Glycosylation	Uniprot
Y413	Phosphorylation	Uniprot

Research Backgrounds

Q9UQV4_LAMP3_HUMAN

Function:

May play a role in dendritic cell function and in adaptive immunity.

Subcellular Location:

Lysosome membrane>Single-pass type I membrane protein. Cytoplasmic vesicle membrane>Single-pass type I membrane protein.
Note: During dendritic cell maturation, detected on cytoplasmic vesicles (the MHC II compartment) that contain MHC II proteins, LAMP1, LAMP2 and LAMP3 (PubMed:9768752). Detected on lysosomes in mature dendritic cells (PubMed:9768752).

Tissue Specificity:

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the LAMP family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

Restrictive clause

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LAMP3 Antibody - #DF7099