DRD1 Antibody - #DF7097

(5)   (3)  
Product: DRD1 Antibody
Catalog: DF7097
Description: Rabbit polyclonal antibody to DRD1
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 49kDa; 49kD(Calculated).
Uniprot: P21728
RRID: AB_2839052

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(90%), Sheep(90%), Dog(90%)
Clonality:
Polyclonal
Specificity:
DRD1 Antibody detects endogenous levels of total DRD1.
RRID:
AB_2839052
Cite Format: Affinity Biosciences Cat# DF7097, RRID:AB_2839052.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D(1A) dopamine receptor; D1A dopamine receptor; DADR; Dopamine D1 receptor; dopamine receptor D1; DR D1; DR D1A; DRD 1; DRD 1A; DRD1; DRD1_HUMAN; DRD1A;

Immunogens

Immunogen:

A synthesized peptide derived from human DRD1, corresponding to a region within N-terminal amino acids.

Uniprot:

P21728(DRD1_HUMAN) >>Visit HPA database.

Gene(ID):
DRD1(1812)
Expression:
P21728 DRD1_HUMAN:

Detected in caudate, nucleus accumbens and in the olfactory tubercle.

Description:
This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
Sequence:
MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISSSVISFYIPVAIMIVTYTRIYRIAQKQIRRIAALERAAVHAKNCQTTTGNGKPVECSQPESSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFILNCILPFCGSGETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRLCPATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
90
Sheep
90
Dog
90
Chicken
78
Horse
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell projection>Dendrite. Cell projection>Dendritic spine.
Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in caudate, nucleus accumbens and in the olfactory tubercle.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Morphine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Nervous system > Dopaminergic synapse.

References

1). Rasd2 regulates depression-like behaviors via DRD2 neurons in the prelimbic cortex afferent to nucleus accumbens core circuit. Molecular psychiatry, 2025 (PubMed: 39097664) [IF=9.6]

Application: WB    Species: Mouse    Sample:

Fig. 2: The mechanisms of 5-d UMS in inducing depression-like behavior involve RASD2 and DRD2. a The representative images showing RASD2 expression in the NAc (top, scale bar = 200 and 20 μm), and the number of RASD2-expressing neurons in NAcc and NAcs (bottom). N = 4 mice per group. Two-tailed unpaired t-test: NAcc, t(19) = 2.418, p = 0.0258; NAcs, t(20) = 2.591, p = 0.0175. b Representative images showing RASD2 expression in the mPFC (top, scale bar = 200 and 20 μm), and the number of RASD2-expressing neurons in Cg1, PrL, and IL (bottom). N = 3 mice per group. Two-tailed unpaired t-test: Cg1, t(10) = 2.721, p = 0.0215; PrL, t(11) = 0.9488, p = 0.3631; IL, t(11) = 3.407, p = 0.0059. c 5-d UMS caused changes in RASD2 and dopamine-related protein expression in the NAc. N = 4–7 mice. Two-tailed unpaired t-test: RASD2, t(7) = 3.050, p = 0.0186; DRD1, t(9) = 2.315, p = 0.0458; DRD2, t(7) = 2.597, p = 0.0356; DARPP-32, t(8) = 2.313, p = 0.0494; GluA1, t(10) = 1.035, p = 0.3251. d 5-d UMS caused changes in RASD2 and dopamine-related protein expression in the mPFC. N = 4–7 mice. Two-tailed unpaired t-test: RASD2, t(8) = 2.507, p = 0.0365; DRD1, t(10) = 0.4337, p = 0.6737; DRD2, t(8) = 0.9415, p = 0.3740; DARPP-32, t(7) = 2.981, p = 0.0205; GluA1, t(10) = 2.250, p = 0.0482. e Western blot images of a RASD2 immunoprecipitation showing co-immunoprecipitation of RASD2 and DRD2 in the NAc (left). Quantification of DRD2 co-immunoprecipitation with RASD2 immunoprecipitation. Each sample was normalized by its input and then DRD2 was normalized for the amount of RASD2 immunoprecipitated (right). N = 5 mice per group. Two-tailed unpaired t-test: t(8) = 6.616, p = 0.0002. f Schematic of experimental design and timeline. g 5-d UMS reduces the projection of PrL to the NAcc (scale bar, 20 μm). N = 4 mice per group. Two-tailed unpaired t-test: Cg1, t(22) = 1.665, p = 0.1102; PrL, t(22) = 2.202, p = 0.0385; IL, t(22) = 0.4018, p = 0.6917. The data are expressed as mean ± s.e.m. *p 
2). Long-life triclosan exposure induces ADHD-like behavior in rats via prefrontal cortex dopaminergic deficiency. Ecotoxicology and environmental safety, 2024 (PubMed: 39047361) [IF=6.2]
3). Cotransplantation of NSCs and ethyl stearate promotes synaptic plasticity in PD rats by Drd1/ERK/AP-1 signaling pathway. Journal of ethnopharmacology, 2024 (PubMed: 37806537) [IF=4.8]
4). Effects of electroacupuncture on the ventral tegmental area- nucleus accumbens dopamine pathway in rats with chronic sleep deprivation. Acupuncture in Medicine, 2023 (PubMed: 36655631) [IF=2.4]
5). Dopamine D2 receptors in the dorsomedial prefrontal cortex modulate social hierarchy in male mice. Current zoology, 2022 (PubMed: 37876636) [IF=1.6]

Application: WB    Species: Mouse    Sample:

Figure 4D1R and D2R expression profiles in the mPFC in different rank groups. (A, B) Representative western-blot images of D1Rs and D2Rs. (C, D) Quantitative analysis of D1R and D2R expression in the mPFC in different rank groups. (E, F) qRT-PCR results showing the relative expression of D1R and D2R mRNA in different rank groups. (G, H) Representative melting curves of D1R and D2R expression during qRT-PCR, showing primer availability and PCR product homogeneity (lines without arrows are β-actin). Panels C–E are analyzed with 1-way ANOVA. Panel F was analyzed with the Kruskal–Wallis test. Data are shown as the mean ± SE; n = 6 in each group; *P < 0.05. dmPFC: dorsal part of the medial prefrontal cortex; vmPFC: ventral part of the medial prefrontal cortex.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.