DRD1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 3T3, using DRD1 Antibody.

Product:	DRD1 Antibody
Catalog:	DF7097
Description:	Rabbit polyclonal antibody to DRD1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	49kDa; 49kD(Calculated).
Uniprot:	P21728
RRID:	AB_2839052

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(90%), Sheep(90%), Dog(90%)

Clonality:

Polyclonal

Specificity:

DRD1 Antibody detects endogenous levels of total DRD1.

RRID:

AB_2839052
Cite Format: Affinity Biosciences Cat# DF7097, RRID:AB_2839052.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

D(1A) dopamine receptor; D1A dopamine receptor; DADR; Dopamine D1 receptor; dopamine receptor D1; DR D1; DR D1A; DRD 1; DRD 1A; DRD1; DRD1_HUMAN; DRD1A;

Immunogens

Immunogen:

Uniprot:

P21728(DRD1_HUMAN) >>Visit HPA database.

Gene(ID):

DRD1(1812)

Expression:

P21728 DRD1_HUMAN:

Detected in caudate, nucleus accumbens and in the olfactory tubercle.

Description:

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.

Sequence:

P21728 DRD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISSSVISFYIPVAIMIVTYTRIYRIAQKQIRRIAALERAAVHAKNCQTTTGNGKPVECSQPESSFKMSFKRETKVLKTLSVIMGVFVCCWLPFFILNCILPFCGSGETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRLCPATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

Sheep

Dog

Chicken

Horse

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P21728 As Substrate

P21728

Site	PTM Type	Source
S56	Phosphorylation	Uniprot
T136	Phosphorylation	Uniprot
K223	Ubiquitination	Uniprot
T360	Phosphorylation	Uniprot

Research Backgrounds

P21728_DRD1_HUMAN

Function:

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell projection>Dendrite. Cell projection>Dendritic spine.
Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.

Tissue Specificity:

Detected in caudate, nucleus accumbens and in the olfactory tubercle.

Subunit Structure:

Interacts with DNAJC14 via its C-terminus (By similarity). Interacts with DRD2 (By similarity).

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Morphine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Nervous system > Dopaminergic synapse.

References

1). Cotransplantation of NSCs and ethyl stearate promotes synaptic plasticity in PD rats by Drd1/ERK/AP-1 signaling pathway. Journal of ethnopharmacology, 2024 (PubMed: 37806537) [IF=5.4]

2). Effects of electroacupuncture on the ventral tegmental area- nucleus accumbens dopamine pathway in rats with chronic sleep deprivation. Acupuncture in Medicine, 2023 (PubMed: 36655631) [IF=2.5]

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DRD1 Antibody - #DF7097