CPT2 Antibody | Affinity Biosciences

IHC
IF/ICC
Cites

1/5

DF7089 at 1/100 staining human liver tissue sections by IHC-P.

DF7089 at 1/100 staining Human colorectal cancer by IHC-P.

DF7089 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

DF7089 staining Hela cells by IF/ICC.

Figure 6 Down-regulation of BMP5 attenuates mitochondrial fat oxidation and triglyceride synthesis.

Product:	CPT2 Antibody
Catalog:	DF7089
Description:	Rabbit polyclonal antibody to CPT2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	74kDa; 74kD(Calculated).
Uniprot:	P23786
RRID:	AB_2839044

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

CPT2 Antibody detects endogenous levels of total CPT2.

RRID:

AB_2839044
Cite Format: Affinity Biosciences Cat# DF7089, RRID:AB_2839044.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Carnitine O palmitoyltransferase 2; Carnitine O palmitoyltransferase 2 mitochondrial; Carnitine O-palmitoyltransferase 2; Carnitine palmitoyltransferase 2; Carnitine palmitoyltransferase II; CPT 1; CPT 2; CPT II; CPT1; CPT2; CPT2_HUMAN; CPTASE; CPTII; IIAE4; mitochondrial;

Immunogens

Immunogen:

Uniprot:

P23786(CPT2_HUMAN) >>Visit HPA database.

Gene(ID):

CPT2(1376)

Description:

The protein encoded by this gene is a nuclear protein which is transported to the mitochondrial inner membrane. Together with carnitine palmitoyltransferase I, the encoded protein oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders.

Sequence:

P23786 CPT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVPRLLLRAWPRGPAVGPGAPSRPLSAGSGPGQYLQRSIVPTMHYQDSLPRLPIPKLEDTIRRYLSAQKPLLNDGQFRKTEQFCKSFENGIGKELHEQLVALDKQNKHTSYISGPWFDMYLSARDSVVLNFNPFMAFNPDPKSEYNDQLTRATNMTVSAIRFLKTLRAGLLEPEVFHLNPAKSDTITFKRLIRFVPSSLSWYGAYLVNAYPLDMSQYFRLFNSTRLPKPSRDELFTDDKARHLLVLRKGNFYIFDVLDQDGNIVSPSEIQAHLKYILSDSSPAPEFPLAYLTSENRDIWAELRQKLMSSGNEESLRKVDSAVFCLCLDDFPIKDLVHLSHNMLHGDGTNRWFDKSFNLIIAKDGSTAVHFEHSWGDGVAVLRFFNEVFKDSTQTPAVTPQSQPATTDSTVTVQKLNFELTDALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKKQKLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVYTKRCSEAFVREPSRHSAGELQQMMVECSKYHGQLTKEAAMGQGFDRHLFALRHLAAAKGIILPELYLDPAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPGRNAREFLQCVEKALEDMFDALEGKSIKS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

Dog

Chicken

Rabbit

Zebrafish

Xenopus

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23786 As Substrate

P23786

Site	PTM Type	Source
T42	Phosphorylation	Uniprot
Y45	Phosphorylation	Uniprot
R51	Methylation	Uniprot
T60	Phosphorylation	Uniprot
K69	Acetylation	Uniprot
K69	Ubiquitination	Uniprot
S126	Phosphorylation	Uniprot
S158	Phosphorylation	Uniprot
K182	Ubiquitination	Uniprot
S197	Phosphorylation	Uniprot
S198	Phosphorylation	Uniprot
Y202	Phosphorylation	Uniprot
S215	Phosphorylation	Uniprot
Y217	Phosphorylation	Uniprot
S223	Phosphorylation	Uniprot
T224	Phosphorylation	Uniprot
Y290	Phosphorylation	Uniprot
S490	Phosphorylation	Uniprot
T491	Phosphorylation	Uniprot
Y508	Phosphorylation	Uniprot
K510	Acetylation	Uniprot
K510	Methylation	Uniprot
S513	Phosphorylation	Uniprot
K544	Acetylation	Uniprot
R554	Methylation	Uniprot

Research Backgrounds

P23786_CPT2_HUMAN

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Family&Domains:

Belongs to the carnitine/choline acetyltransferase family.

Research Fields

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

References

1). Dioscin ameliorates murine ulcerative colitis by regulating macrophage polarization. Pharmacological research, 2021 (PubMed: 34343656) [IF=9.3]

2). Mitochondrial dysfunction resulting from the down-regulation of bone morphogenetic protein 5 may cause microtia. Annals of Translational Medicine, 2021 (PubMed: 33842639)

Application: WB Species: Human Sample: 293T cells

Figure 6 Down-regulation of BMP5 attenuates mitochondrial fat oxidation and triglyceride synthesis. (A) The expression of CPT2 mRNA in 293T cells treated with BMP5-siRNA shows a larger decrease than that in the siNC group. (B) The expression of CPT2 mRNA in ADSCs treated with BMP5-siRNA decreased. (C) CPT2 protein expression in 293T cells treated with BMP5-siRNA shows a larger decrease than that in the siNC group. (D) The expression of DGAT2 mRNA in 293T cells treated with BMP5-siRNA decreased. (E) The expression of DGAT2 mRNA in ADSCs treated with BMP5-siRNA decreased. (F) DGAT2 protein expression in 293T cells treated with BMP5-siRNA decreased (G) Representative western blots of CPT2 and DGAT2 enzymes in 3 groups of 293T cells. *, P<0.05; **, P<0.01. BMP5, bone morphogenetic protein 5; ADSC, adipose-derived stem cells; NC, normal control; CPT2, carnitine O-palmitoyltransferase 2; DGAT2, diacylglycerol acyltransferase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CPT2 Antibody - #DF7089