TRAP1 Antibody - #DF7073

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Product: TRAP1 Antibody
Catalog: DF7073
Description: Rabbit polyclonal antibody to TRAP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 80kDa; 80kD(Calculated).
Uniprot: Q12931
RRID: AB_2839029

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(90%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TRAP1 Antibody detects endogenous levels of total TRAP1.
RRID:
AB_2839029
Cite Format: Affinity Biosciences Cat# DF7073, RRID:AB_2839029.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Heat shock protein 75 kDa; Heat shock protein 75 kDa, mitochondrial; HSP 75; HSP75; HSP90L; mitochondrial; TNF receptor associated protein 1; TNFR-associated protein 1; TRAP-1; Trap1; TRAP1_HUMAN; Tumor necrosis factor type 1 receptor-associated protein;

Immunogens

Immunogen:
Uniprot:

Q12931(TRAP1_HUMAN) >>Visit HPA database.

Gene(ID):
TRAP1(10131)
Expression:
Q12931 TRAP1_HUMAN:

Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Expression is highly reduced in bladder cancer and renal cell carcinoma specimens compared to healthy tissues, but it is increased in other type of tumors.

Description:
This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.
Sequence:
MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDALEKLRHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNFVSFPLYLNGRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
90
Chicken
90
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12931 As Substrate

Site PTM Type Enzyme
T39 Phosphorylation
T40 Phosphorylation
K109 Acetylation
K109 Ubiquitination
S118 Phosphorylation
S121 Phosphorylation
K126 Acetylation
S194 Phosphorylation
S236 Phosphorylation
K324 Acetylation
K324 Ubiquitination
Y331 Phosphorylation
K332 Acetylation
K332 Ubiquitination
T333 Phosphorylation
S341 Phosphorylation
Y344 Phosphorylation
Y366 Phosphorylation
K382 Acetylation
K382 Ubiquitination
R388 Methylation
S393 Phosphorylation
S401 Phosphorylation
K424 Acetylation
K431 Acetylation
K432 Acetylation
Y444 Phosphorylation
K461 Ubiquitination
K466 Acetylation
Y486 Phosphorylation
T494 Phosphorylation
Y498 Phosphorylation
Y499 Phosphorylation
C501 S-Nitrosylation
R505 Methylation
S511 Phosphorylation
Y513 Phosphorylation
Y514 Phosphorylation
K518 Acetylation
R567 Methylation
S568 Phosphorylation
C573 S-Nitrosylation
K652 Acetylation
K699 Ubiquitination

Research Backgrounds

Function:

Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.

Subcellular Location:

Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Expression is highly reduced in bladder cancer and renal cell carcinoma specimens compared to healthy tissues, but it is increased in other type of tumors.

Subunit Structure:

Binds to the intracellular domain of tumor necrosis factor type 1 receptor. Binds to RB1. Interacts with SRC. Interacts with SDHA.

Family&Domains:

Belongs to the heat shock protein 90 family.

References

1). AMPK activation attenuates cancer-induced bone pain by reducing mitochondrial dysfunction-mediated neuroinflammation: AMPK activation attenuates bone cancer pain. ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2023 (PubMed: 36971458) [IF=3.3]

Application: IF/ICC    Species: Rat    Sample: spinal cord

Figure 3 Effect of AICAR treatment on the activities of AMPK and Drp1 (A,B) Representative immunofluorescence staining images of the expression of AMPK and Drp1 in the spinal dorsal horn of sham and CIBP rats. Scale bar: 20 μm. (C) Quantitative analysis of fluorescence intensity in (A) and (B). Data are expressed as the mean±SD ( n=3). * P

Application: WB    Species: Rat    Sample: spinal cord

Figure 3 Effect of AICAR treatment on the activities of AMPK and Drp1 (A,B) Representative immunofluorescence staining images of the expression of AMPK and Drp1 in the spinal dorsal horn of sham and CIBP rats. Scale bar: 20 μm. (C) Quantitative analysis of fluorescence intensity in (A) and (B). Data are expressed as the mean±SD ( n=3). * P

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