GNA11 Antibody - #DF7067

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Product: GNA11 Antibody
Catalog: DF7067
Description: Rabbit polyclonal antibody to GNA11
Application: WB IHC
Reactivity: Human, Mouse, Rat, Pig
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 42kDa; 42kD(Calculated).
Uniprot: P29992
RRID: AB_2839023

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Pig
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GNA11 Antibody detects endogenous levels of total GNA11.
RRID:
AB_2839023
Cite Format: Affinity Biosciences Cat# DF7067, RRID:AB_2839023.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G alpha 11; G alpha q; G protein subunit alpha 11; GAQ; GNA 11; GNA11; GNAQ; Guanine nucleotide binding protein (G protein) alpha 11 (Gq class); Guanine nucleotide binding protein (G protein) alpha 11; Guanine nucleotide binding protein (G protein) q polypeptide; Guanine nucleotide binding protein alpha q; Guanine nucleotide binding protein G(q) subunit alpha; Guanine nucleotide binding protein G(y) subunit alpha; Guanine nucleotide binding protein Gq class GNA11; Guanine nucleotide binding protein subunit alpha 11;

Immunogens

Immunogen:
Uniprot:

P29992(GNA11_HUMAN) >>Visit HPA database.

Gene(ID):
GNA11(2767)
Expression:
P29992 GNA11_HUMAN:

Expressed in testis.

Description:
Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).
Sequence:
MTLESMMACCLSDEVKESKRINAEIEKQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGAGYSEEDKRGFTKLVYQNIFTAMQAMIRAMETLKILYKYEQNKANALLIREVDVEKVTTFEHQYVSAIKTLWEDPGIQECYDRRREYQLSDSAKYYLTDVDRIATLGYLPTQQDVLRVRVPTTGIIEYPFDLENIIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEDKILYSHLVDYFPEFDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29992 As Substrate

Site PTM Type Enzyme
K16 Ubiquitination
R20 Methylation
K27 Ubiquitination
S50 Phosphorylation
K52 Ubiquitination
S68 Phosphorylation
K72 Ubiquitination
K98 Ubiquitination
K102 Sumoylation
K102 Ubiquitination
K107 Ubiquitination
K120 Ubiquitination
K133 Ubiquitination
C144 S-Nitrosylation
S154 Phosphorylation
K158 Ubiquitination
T162 Phosphorylation
T169 Phosphorylation
K252 Ubiquitination
K282 Ubiquitination
Y291 Phosphorylation
K345 Ubiquitination
K354 Ubiquitination

Research Backgrounds

Function:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C.

PTMs:

(Microbial infection) Deamidated at Gln-209 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.

Subcellular Location:

Cell membrane>Lipid-anchor. Cytoplasm.
Note: In testicular cells, expressed exclusively in the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in testis.

Subunit Structure:

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RGS22. Interacts with NTSR1.

Family&Domains:

Belongs to the G-alpha family. G(q) subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

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