CA4 Antibody | Affinity Biosciences

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Western blot analysis of extracts from mouse fetal lung tissue lysates, using CA4 antibody.

Product:	CA4 Antibody
Catalog:	DF7053
Description:	Rabbit polyclonal antibody to CA4
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Rabbit
Mol.Wt.:	35kDa; 35kD(Calculated).
Uniprot:	P22748
RRID:	AB_2839009

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(86%), Rabbit(88%)

Clonality:

Polyclonal

Specificity:

CA4 Antibody detects endogenous levels of total CA4.

RRID:

AB_2839009
Cite Format: Affinity Biosciences Cat# DF7053, RRID:AB_2839009.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CA IV; CA4; CAH4_HUMAN; CAIV; Car4; Carbonate dehydratase IV; Carbonic anhydrase 4; Carbonic dehydratase; Carbonic dehydratase IV; EC 4.2.1.1; Retinitis pigmentosa 17 (autosomal dominant); RP17;

Immunogens

Immunogen:

Uniprot:

P22748(CAH4_HUMAN) >>Visit HPA database.

Gene(ID):

CA4(762)

Expression:

P22748 CAH4_HUMAN:

Expressed in the endothelium of the choriocapillaris in eyes (at protein level). Not expressed in the retinal epithelium at detectable levels.

Description:

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008]

Sequence:

P22748 CAH4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRMLLALLALSAARPSASAESHWCYEVQAESSNYPCLVPVKWGGNCQKDRQSPINIVTTKAKVDKKLGRFFFSGYDKKQTWTVQNNGHSVMMLLENKASISGGGLPAPYQAKQLHLHWSDLPYKGSEHSLDGEHFAMEMHIVHEKEKGTSRNVKEAQDPEDEIAVLAFLVEAGTQVNEGFQPLVEALSNIPKPEMSTTMAESSLLDLLPKEEKLRHYFRYLGSLTTPTCDEKVVWTVFREPIQLHREQILAFSQKLYYDKEQTVSMKDNVRPLQQLGQRTVIKSGAPGRPLPWALPALLGPMLACLLAGFLR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Bovine

Horse

Chicken

Dog

Xenopus

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22748 As Substrate

P22748

Site	PTM Type	Source
T80	Phosphorylation	Uniprot
T82	Phosphorylation	Uniprot
S89	Phosphorylation	Uniprot
T198	Phosphorylation	Uniprot

Research Backgrounds

P22748_CAH4_HUMAN

Function:

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.

Subcellular Location:

Cell membrane>Lipid-anchor.

Tissue Specificity:

Expressed in the endothelium of the choriocapillaris in eyes (at protein level). Not expressed in the retinal epithelium at detectable levels.

Subunit Structure:

Interacts with SLC4A4.

Family&Domains:

Belongs to the alpha-carbonic anhydrase family.

Research Fields

· Metabolism > Energy metabolism > Nitrogen metabolism.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

Restrictive clause

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For Research Use Only.
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CA4 Antibody - #DF7053