Cytokeratin 4 Antibody - #DF7050

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Product: Cytokeratin 4 Antibody
Catalog: DF7050
Description: Rabbit polyclonal antibody to Cytokeratin 4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit
Mol.Wt.: 57kDa; 56kD(Calculated).
Uniprot: P19013
RRID: AB_2839006

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Horse(92%), Sheep(92%), Rabbit(92%)
Clonality:
Polyclonal
Specificity:
Cytokeratin 4 Antibody detects endogenous levels of total Cytokeratin 4.
RRID:
AB_2839006
Cite Format: Affinity Biosciences Cat# DF7050, RRID:AB_2839006.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK 4; CK-4; CK4; CYK4; Cytokeratin 4; Cytokeratin-4; Cytokeratin4; FLJ31692; K2C4_HUMAN; K4; Keratin 4; Keratin; Keratin type II cytoskeletal 4; Keratin-4; Keratin4; KRT 4; Krt4; type II cytoskeletal 4; Type-II keratin Kb4;

Immunogens

Immunogen:
Uniprot:

P19013(K2C4_HUMAN) >>Visit HPA database.

Expression:
P19013 K2C4_HUMAN:

Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.

Description:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Sequence:
MIARQQCVRGGPRGFSCGSAIVGGGKRGAFSSVSMSGGAGRCSSGGFGSRSLYNLRGNKSISMSVAGSRQGACFGGAGGFGTGGFGGGFGGSFSGKGGPGFPVCPAGGIQEVTINQSLLTPLHVEIDPEIQKVRTEEREQIKLLNNKFASFIDKVQFLEQQNKVLETKWNLLQQQTTTTSSKNLEPLFETYLSVLRKQLDTLGNDKGRLQSELKTMQDSVEDFKTKYEEEINKRTAAENDFVVLKKDVDAAYLNKVELEAKVDSLNDEINFLKVLYDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVRAQYEEIAQRSKAEAEALYQTKVQQLQISVDQHGDNLKNTKSEIAELNRMIQRLRAEIENIKKQCQTLQVSVADAEQRGENALKDAHSKRVELEAALQQAKEELARMLREYQELMSVKLALDIEIATYRKLLEGEEYRMSGECQSAVSISVVSGSTSTGGISGGLGSGSGFGLSSGFGSGSGSGFGFGGSVSGSSSSKIISTTTLNKRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Sheep
92
Rabbit
92
Pig
83
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19013 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation
S19 Phosphorylation
K26 Acetylation
S31 Phosphorylation
S32 Phosphorylation
S34 Phosphorylation
S36 Phosphorylation
S49 Phosphorylation
S51 Phosphorylation
Y53 Phosphorylation
S60 Phosphorylation
S64 Phosphorylation
S68 Phosphorylation
K161 Ubiquitination
S164 Phosphorylation
K168 Ubiquitination
Y241 Phosphorylation
K259 Acetylation
Y266 Phosphorylation
K269 Acetylation
Y329 Phosphorylation
Y344 Phosphorylation
T346 Phosphorylation
S413 Phosphorylation
Y436 Phosphorylation
S441 Phosphorylation
Y462 Phosphorylation

Research Backgrounds

Tissue Specificity:

Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13.

Family&Domains:

Belongs to the intermediate filament family.

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