Cytokeratin 15 Antibody - #DF7049

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Product: Cytokeratin 15 Antibody
Catalog: DF7049
Description: Rabbit polyclonal antibody to Cytokeratin 15
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep
Mol.Wt.: 49kDa; 49kD(Calculated).
Uniprot: P19012
RRID: AB_2839005

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%)
Clonality:
Polyclonal
Specificity:
Cytokeratin 15 Antibody detects endogenous levels of total Cytokeratin 15.
RRID:
AB_2839005
Cite Format: Affinity Biosciences Cat# DF7049, RRID:AB_2839005.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI528832; CK 15; CK-15; CK15; Cytokeratin-15; Cytokeratin15; K15; K1C15_HUMAN; K1CO; Ka15; Keratin 15; Keratin 15 basic; Keratin 15 beta; Keratin; Keratin complex 1 acidic gene 15; Keratin type I cytoskeletal 15; Keratin-15; Keratin15; KRT 15; Krt1-15; KRT15; KRTB; KRTL15; Type I cytoskeletal 15; Type I keratin Ka15;

Immunogens

Immunogen:
Uniprot:

P19012(K1C15_HUMAN) >>Visit HPA database.

Gene(ID):
KRT15(3866)
Expression:
P19012 K1C15_HUMAN:

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.

Description:
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2.this gene.
Sequence:
MTTTFLQTSSSTFGGGSTRGGSLLAGGGGFGGGSLSGGGGSRSISASSARFVSSGSGGGYGGGMRVCGFGGGAGSVFGGGFGGGVGGGFGGGFGGGDGGLLSGNEKITMQNLNDRLASYLDKVRALEEANADLEVKIHDWYQKQTPTSPECDYSQYFKTIEELRDKIMATTIDNSRVILEIDNARLAADDFRLKYENELALRQGVEADINGLRRVLDELTLARTDLEMQIEGLNEELAYLKKNHEEEMKEFSSQLAGQVNVEMDAAPGVDLTRVLAEMREQYEAMAEKNRRDVEAWFFSKTEELNKEVASNTEMIQTSKTEITDLRRTMQELEIELQSQLSMKAGLENSLAETECRYATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGIAIREASSGGGGSSSNFHINVEESVDGQVVSSHKREI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19012 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation
S17 Phosphorylation
S22 Phosphorylation
S34 Phosphorylation
S36 Phosphorylation
S41 Phosphorylation
S43 Phosphorylation
S45 Phosphorylation
S53 Phosphorylation
Y60 Phosphorylation
S75 Phosphorylation
S118 Phosphorylation
Y119 Phosphorylation
K122 Acetylation
K122 Ubiquitination
Y141 Phosphorylation
T145 Phosphorylation
S148 Phosphorylation
Y195 Phosphorylation
Y239 Phosphorylation
K241 Sumoylation
K241 Ubiquitination
K300 Methylation
T328 Phosphorylation
S349 Phosphorylation
Y389 Phosphorylation
T405 Phosphorylation
Y406 Phosphorylation
S426 Phosphorylation

Research Backgrounds

Tissue Specificity:

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Interacts with NOD2.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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