NRG4 Antibody - #DF7046

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Product: NRG4 Antibody
Catalog: DF7046
Description: Rabbit polyclonal antibody to NRG4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep
Mol.Wt.: 12kDa; 13kD(Calculated).
Uniprot: Q8WWG1
RRID: AB_2839002

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(89%), Sheep(88%)
Clonality:
Polyclonal
Specificity:
NRG4 Antibody detects endogenous levels of total NRG4.
RRID:
AB_2839002
Cite Format: Affinity Biosciences Cat# DF7046, RRID:AB_2839002.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp779N0541; DKFZp779N1944; Heregulin 4; HRG4; Neuregulin 4; Neuregulin-4; NRG-4; NRG4; NRG4_HUMAN; pro-neuregulin-4, membrane-bound isoform; Pro-NRG4;

Immunogens

Immunogen:
Uniprot:

Q8WWG1(NRG4_HUMAN) >>Visit HPA database.

Gene(ID):
NRG4(145957)
Description:
The neuregulins, including NRG4, activate type-1 growth factor receptors (see EGFR; MIM 131550) to initiating cell-to-cell signaling through tyrosine phosphorylation (Harari et al., 1999 [PubMed 10348342]).
Sequence:
MPTDHEEPCGPSHKSFCLNGGLCYVIPTIPSPFCRCVENYTGARCEEVFLPGSSIQTKSNLFEAFVALAVLVTLIIGAFYFLCRKGHFQRASSVQYDINLVETSSTSAHHSHEQH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
89
Sheep
88
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors (By similarity).

PTMs:

Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.

Extensive glycosylation precedes the proteolytic cleavage.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.
Note: Does not seem to be active.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ERBB4.

Family&Domains:

The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization (By similarity).

ERBB receptor binding is elicited entirely by the EGF-like domain.

Belongs to the neuregulin family.

Research Fields

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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